##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI Asp718I AvaI
AviII AvrII BamHI BbsI BcgI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrDI BstBI BstEII BstPI
BstXI CelII ClaI CpoI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MluI MroI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AflIII 2210 ACATGT A^C[GA][CT]GT
AhdI 2209 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1061 GTGCAC G^TGCAC
AocI 2337 CCTGAGG CC^T[ACGT]AGG
ApaI 2746 GGGCCC GGGCC^C
ApaLI 1061 GTGCAC G^TGCAC
AseI 720 ATTAAT AT^TAAT
AsnI 720 ATTAAT AT^TAAT
Asp700I 2773 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2209 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 965 TGGCCA TGG^CCA
BbeI 178 GGCGCC GGCGC^C
BbrPI 165 CACGTG CAC^GTG
BbvCI 2538 CCTCAGC CCTCAGC(-5/-2)
BciVI 781 GTATCC GTATCC(6/5)
BclI 830 TGATCA T^GATCA
BsaAI 165 CACGTG [CT]AC^GT[GA]
BsmI 583 GCATTC GAATGC(1/-1)
BspHI 2806 TCATGA T^CATGA
BspLU11I 2210 ACATGT A^CATGT
BsrFI 197 ACCGGC [GA]^CCGG[CT]
BssHII 2728 GCGCGC G^CGCGC
BssSI 409 CTCGTG CACGAG(-5/-1)
Bst1107I 2396 GTATAC GTA^TAC
BstAPI 900 GCACTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2396 GTATAC GTA^TAC
Bsu36I 2337 CCTGAGG CC^T[ACGT]AGG
Cfr10I 197 ACCGGC [GA]^CCGG[CT]
DraIII 901 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 337 GACGTAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2209 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2337 CCTGAGG CC^T[ACGT]AGG
FauI 43 CCCGC CCCGC(4/6)
FbaI 830 TGATCA T^GATCA
KasI 178 GGCGCC G^GCGCC
KspI 79 CCGCGG CCGC^GG
MfeI 362 CAATTG C^AATTG
MluNI 965 TGGCCA TGG^CCA
MscI 965 TGGCCA TGG^CCA
MslI 1052 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 362 CAATTG C^AATTG
NarI 178 GGCGCC GG^CGCC
NdeI 2330 CATATG CA^TATG
PmaCI 165 CACGTG CAC^GTG
PmlI 165 CACGTG CAC^GTG
PshBI 720 ATTAAT AT^TAAT
PstI 2476 CTGCAG CTGCA^G
PvuII 609 CAGCTG CAG^CTG
RcaI 2806 TCATGA T^CATGA
SacII 79 CCGCGG CCGC^GG
SexAI 3034 ACCAGGT A^CC[AT]GGT
SfoI 178 GGCGCC GGC^GCC
SphI 692 GCATGC GCATG^C
TaqI 159 TCGA T^CGA
TthHB8I 159 TCGA T^CGA
XbaI 2644 TCTAGA T^CTAGA
XcmI 401 CCATCTCTCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2773 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 178 GGCGCC G[GA]^CG[CT]C
2248 GACGCC
AspI 988 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3147 GACCTTGTC
BglII 1704 AGATCT A^GATCT
2879 AGATCT
BsaHI 178 GGCGCC G[GA]^CG[CT]C
2248 GACGCC
Bsp1407I 849 TGTACA T^GTACA
2202 TGTACA
BspMI 749 GCAGGT ACCTGC(4/8)
3081 GCAGGT
BsrGI 849 TGTACA T^GTACA
2202 TGTACA
DraI 2030 TTTAAA TTT^AAA
2998 TTTAAA
EaeI 68 CGGCCA [CT]^GGCC[GA]
965 TGGCCA
EarI 313 CTCTTC CTCTTC(1/4)
1136 GAAGAG
EcoT14I 251 CCATGG C^C[AT][AT]GG
2617 CCATGG
HgaI 1282 GACGC GACGC(5/10)
2248 GACGC
Hin1I 178 GGCGCC G[GA]^CG[CT]C
2248 GACGCC
Ksp632I 313 CTCTTC CTCTTC(1/4)
1136 GAAGAG
MspA1I 79 CCGCGG C[AC]G^C[GT]G
609 CAGCTG
NcoI 251 CCATGG C^CATGG
2617 CCATGG
NheI 2051 GCTAGC G^CTAGC
2186 GCTAGC
PflFI 988 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3147 GACCTTGTC
PpuMI 1611 AGGACCT [GA]G^G[AT]CC[CT]
2596 GGGACCT
SmlI 456 CTCAAG C^T[CT][GA]AG
2420 CTTGAG
SspBI 849 TGTACA T^GTACA
2202 TGTACA
StyI 251 CCATGG C^C[AT][AT]GG
2617 CCATGG
Tth111I 988 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3147 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StuI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII