################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Aor51HI AscI Asp718I AvaI AviII AvrII BamHI BbsI BcgI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrDI BstBI BstEII BstPI BstXI CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MamI MluI MroI NaeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflIII 2210 ACATGT A^C[GA][CT]GT AhdI 2209 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1061 GTGCAC G^TGCAC AocI 2337 CCTGAGG CC^T[ACGT]AGG ApaI 2746 GGGCCC GGGCC^C ApaLI 1061 GTGCAC G^TGCAC AseI 720 ATTAAT AT^TAAT AsnI 720 ATTAAT AT^TAAT Asp700I 2773 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2209 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 965 TGGCCA TGG^CCA BbeI 178 GGCGCC GGCGC^C BbrPI 165 CACGTG CAC^GTG BbvCI 2538 CCTCAGC CCTCAGC(-5/-2) BciVI 781 GTATCC GTATCC(6/5) BclI 830 TGATCA T^GATCA BsaAI 165 CACGTG [CT]AC^GT[GA] BsmI 583 GCATTC GAATGC(1/-1) BspHI 2806 TCATGA T^CATGA BspLU11I 2210 ACATGT A^CATGT BsrFI 197 ACCGGC [GA]^CCGG[CT] BssHII 2728 GCGCGC G^CGCGC BssSI 409 CTCGTG CACGAG(-5/-1) Bst1107I 2396 GTATAC GTA^TAC BstAPI 900 GCACTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2396 GTATAC GTA^TAC Bsu36I 2337 CCTGAGG CC^T[ACGT]AGG Cfr10I 197 ACCGGC [GA]^CCGG[CT] DraIII 901 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 337 GACGTAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2209 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2337 CCTGAGG CC^T[ACGT]AGG FauI 43 CCCGC CCCGC(4/6) FbaI 830 TGATCA T^GATCA KasI 178 GGCGCC G^GCGCC KspI 79 CCGCGG CCGC^GG MfeI 362 CAATTG C^AATTG MluNI 965 TGGCCA TGG^CCA MscI 965 TGGCCA TGG^CCA MslI 1052 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 362 CAATTG C^AATTG NarI 178 GGCGCC GG^CGCC NdeI 2330 CATATG CA^TATG PmaCI 165 CACGTG CAC^GTG PmlI 165 CACGTG CAC^GTG PshBI 720 ATTAAT AT^TAAT PstI 2476 CTGCAG CTGCA^G PvuII 609 CAGCTG CAG^CTG RcaI 2806 TCATGA T^CATGA SacII 79 CCGCGG CCGC^GG SexAI 3034 ACCAGGT A^CC[AT]GGT SfoI 178 GGCGCC GGC^GCC SphI 692 GCATGC GCATG^C TaqI 159 TCGA T^CGA TthHB8I 159 TCGA T^CGA XbaI 2644 TCTAGA T^CTAGA XcmI 401 CCATCTCTCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2773 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 178 GGCGCC G[GA]^CG[CT]C 2248 GACGCC AspI 988 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3147 GACCTTGTC BglII 1704 AGATCT A^GATCT 2879 AGATCT BsaHI 178 GGCGCC G[GA]^CG[CT]C 2248 GACGCC Bsp1407I 849 TGTACA T^GTACA 2202 TGTACA BspMI 749 GCAGGT ACCTGC(4/8) 3081 GCAGGT BsrGI 849 TGTACA T^GTACA 2202 TGTACA DraI 2030 TTTAAA TTT^AAA 2998 TTTAAA EaeI 68 CGGCCA [CT]^GGCC[GA] 965 TGGCCA EarI 313 CTCTTC CTCTTC(1/4) 1136 GAAGAG EcoT14I 251 CCATGG C^C[AT][AT]GG 2617 CCATGG HgaI 1282 GACGC GACGC(5/10) 2248 GACGC Hin1I 178 GGCGCC G[GA]^CG[CT]C 2248 GACGCC Ksp632I 313 CTCTTC CTCTTC(1/4) 1136 GAAGAG MspA1I 79 CCGCGG C[AC]G^C[GT]G 609 CAGCTG NcoI 251 CCATGG C^CATGG 2617 CCATGG NheI 2051 GCTAGC G^CTAGC 2186 GCTAGC PflFI 988 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3147 GACCTTGTC PpuMI 1611 AGGACCT [GA]G^G[AT]CC[CT] 2596 GGGACCT SmlI 456 CTCAAG C^T[CT][GA]AG 2420 CTTGAG SspBI 849 TGTACA T^GTACA 2202 TGTACA StyI 251 CCATGG C^C[AT][AT]GG 2617 CCATGG Tth111I 988 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3147 GACCTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StuI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII