##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Aor51HI     AscI        Asp718I     AvaI        
AviII       AvrII       BamHI       BbsI        BcgI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BsrBI       BsrDI       BstBI       BstEII      BstPI       
BstXI       CelII       ClaI        CpoI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
MamI        MluI        MroI        NaeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AflIII       2210  ACATGT               A^C[GA][CT]GT
AhdI         2209  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1061  GTGCAC               G^TGCAC
AocI         2337  CCTGAGG              CC^T[ACGT]AGG
ApaI         2746  GGGCCC               GGGCC^C
ApaLI        1061  GTGCAC               G^TGCAC
AseI          720  ATTAAT               AT^TAAT
AsnI          720  ATTAAT               AT^TAAT
Asp700I      2773  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2209  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          965  TGGCCA               TGG^CCA
BbeI          178  GGCGCC               GGCGC^C
BbrPI         165  CACGTG               CAC^GTG
BbvCI        2538  CCTCAGC              CCTCAGC(-5/-2)
BciVI         781  GTATCC               GTATCC(6/5)
BclI          830  TGATCA               T^GATCA
BsaAI         165  CACGTG               [CT]AC^GT[GA]
BsmI          583  GCATTC               GAATGC(1/-1)
BspHI        2806  TCATGA               T^CATGA
BspLU11I     2210  ACATGT               A^CATGT
BsrFI         197  ACCGGC               [GA]^CCGG[CT]
BssHII       2728  GCGCGC               G^CGCGC
BssSI         409  CTCGTG               CACGAG(-5/-1)
Bst1107I     2396  GTATAC               GTA^TAC
BstAPI        900  GCACTAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      2396  GTATAC               GTA^TAC
Bsu36I       2337  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        197  ACCGGC               [GA]^CCGG[CT]
DraIII        901  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          337  GACGTAGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2209  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2337  CCTGAGG              CC^T[ACGT]AGG
FauI           43  CCCGC                CCCGC(4/6)
FbaI          830  TGATCA               T^GATCA
KasI          178  GGCGCC               G^GCGCC
KspI           79  CCGCGG               CCGC^GG
MfeI          362  CAATTG               C^AATTG
MluNI         965  TGGCCA               TGG^CCA
MscI          965  TGGCCA               TGG^CCA
MslI         1052  CATACTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          362  CAATTG               C^AATTG
NarI          178  GGCGCC               GG^CGCC
NdeI         2330  CATATG               CA^TATG
PmaCI         165  CACGTG               CAC^GTG
PmlI          165  CACGTG               CAC^GTG
PshBI         720  ATTAAT               AT^TAAT
PstI         2476  CTGCAG               CTGCA^G
PvuII         609  CAGCTG               CAG^CTG
RcaI         2806  TCATGA               T^CATGA
SacII          79  CCGCGG               CCGC^GG
SexAI        3034  ACCAGGT              A^CC[AT]GGT
SfoI          178  GGCGCC               GGC^GCC
SphI          692  GCATGC               GCATG^C
TaqI          159  TCGA                 T^CGA
TthHB8I       159  TCGA                 T^CGA
XbaI         2644  TCTAGA               T^CTAGA
XcmI          401  CCATCTCTCTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2773  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          178  GGCGCC               G[GA]^CG[CT]C
             2248  GACGCC              
AspI          988  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3147  GACCTTGTC           
BglII        1704  AGATCT               A^GATCT
             2879  AGATCT              
BsaHI         178  GGCGCC               G[GA]^CG[CT]C
             2248  GACGCC              
Bsp1407I      849  TGTACA               T^GTACA
             2202  TGTACA              
BspMI         749  GCAGGT               ACCTGC(4/8)
             3081  GCAGGT              
BsrGI         849  TGTACA               T^GTACA
             2202  TGTACA              
DraI         2030  TTTAAA               TTT^AAA
             2998  TTTAAA              
EaeI           68  CGGCCA               [CT]^GGCC[GA]
              965  TGGCCA              
EarI          313  CTCTTC               CTCTTC(1/4)
             1136  GAAGAG              
EcoT14I       251  CCATGG               C^C[AT][AT]GG
             2617  CCATGG              
HgaI         1282  GACGC                GACGC(5/10)
             2248  GACGC               
Hin1I         178  GGCGCC               G[GA]^CG[CT]C
             2248  GACGCC              
Ksp632I       313  CTCTTC               CTCTTC(1/4)
             1136  GAAGAG              
MspA1I         79  CCGCGG               C[AC]G^C[GT]G
              609  CAGCTG              
NcoI          251  CCATGG               C^CATGG
             2617  CCATGG              
NheI         2051  GCTAGC               G^CTAGC
             2186  GCTAGC              
PflFI         988  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3147  GACCTTGTC           
PpuMI        1611  AGGACCT              [GA]G^G[AT]CC[CT]
             2596  GGGACCT             
SmlI          456  CTCAAG               C^T[CT][GA]AG
             2420  CTTGAG              
SspBI         849  TGTACA               T^GTACA
             2202  TGTACA              
StyI          251  CCATGG               C^C[AT][AT]GG
             2617  CCATGG              
Tth111I       988  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3147  GACCTTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspI        
StuI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XhoII