##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI ApaI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AhdI 283 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1095 GTGCAC G^TGCAC
Aor51HI 386 AGCGCT AGC^GCT
ApaLI 1095 GTGCAC G^TGCAC
AspEI 283 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 575 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2336 GGATCC G^GATCC
BcgI 2139 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2072 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1897 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1271 GAGACC GGTCTC(1/5)
BsaWI 637 TCCGGT [AT]^CCGG[AT]
BsmBI 13 CGTCTC CGTCTC(1/5)
BsrBI 113 CCGCTC CCGCTC(-3/-3)
BsrFI 1274 ACCGGC [GA]^CCGG[CT]
BstXI 871 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1274 ACCGGC [GA]^CCGG[CT]
DrdI 181 GACAAGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 283 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 386 AGCGCT AGC^GCT
EcoNI 1887 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 386 AGCGCT [GA]GCGC^[CT]
HincII 1724 GTTGAC GT[CT]^[GA]AC
HindII 1724 GTTGAC GT[CT]^[GA]AC
MamI 1897 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1938 CATATG CA^TATG
NspI 1296 GCATGC [GA]CATG^[CT]
PaeR7I 1985 CTCGAG C^TCGAG
PflFI 575 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1741 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1366 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 710 GAGCTC GAGCT^C
SapI 1341 GAAGAGC GCTCTTC(1/4)
SexAI 1332 ACCAGGT A^CC[AT]GGT
SmlI 1985 CTCGAG C^T[CT][GA]AG
SphI 1296 GCATGC GCATG^C
SspI 2308 AATATT AAT^ATT
Tth111I 575 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1741 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1737 CCATCCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1985 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 315 GTCTAC GT^[AC][GT]AC
3045 GTCTAC
AocI 128 CCTCAGG CC^T[ACGT]AGG
2331 CCTGAGG
Asp700I 906 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2813 GAACCTTTTC
BanI 204 GGTGCC G^G[CT][GA]CC
2796 GGTGCC
BbsI 425 GAAGAC GAAGAC(2/6)
662 GAAGAC
BbvCI 686 GCTGAGG CCTCAGC(-5/-2)
1657 GCTGAGG
BpuAI 425 GAAGAC GAAGAC(2/6)
662 GAAGAC
Bsp1407I 1590 TGTACA T^GTACA
2314 TGTACA
BsrGI 1590 TGTACA T^GTACA
2314 TGTACA
BstAPI 27 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1249 GCAAGCCATGC
BstYI 552 GGATCT [GA]^GATC[CT]
2336 GGATCC
Bsu36I 128 CCTCAGG CC^T[ACGT]AGG
2331 CCTGAGG
DraI 2241 TTTAAA TTT^AAA
2445 TTTAAA
Eco81I 128 CCTCAGG CC^T[ACGT]AGG
2331 CCTGAGG
EcoRI 379 GAATTC G^AATTC
1357 GAATTC
MflI 552 GGATCT [GA]^GATC[CT]
2336 GGATCC
PpuMI 651 AGGACCC [GA]G^G[AT]CC[CT]
1674 GGGACCT
PstI 1824 CTGCAG CTGCA^G
2960 CTGCAG
SspBI 1590 TGTACA T^GTACA
2314 TGTACA
XhoII 552 GGATCT [GA]^GATC[CT]
2336 GGATCC
XmnI 906 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2813 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI