################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI ApaI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AhdI 283 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1095 GTGCAC G^TGCAC Aor51HI 386 AGCGCT AGC^GCT ApaLI 1095 GTGCAC G^TGCAC AspEI 283 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 575 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2336 GGATCC G^GATCC BcgI 2139 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2072 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1897 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1271 GAGACC GGTCTC(1/5) BsaWI 637 TCCGGT [AT]^CCGG[AT] BsmBI 13 CGTCTC CGTCTC(1/5) BsrBI 113 CCGCTC CCGCTC(-3/-3) BsrFI 1274 ACCGGC [GA]^CCGG[CT] BstXI 871 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1274 ACCGGC [GA]^CCGG[CT] DrdI 181 GACAAGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 283 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 386 AGCGCT AGC^GCT EcoNI 1887 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 386 AGCGCT [GA]GCGC^[CT] HincII 1724 GTTGAC GT[CT]^[GA]AC HindII 1724 GTTGAC GT[CT]^[GA]AC MamI 1897 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 1938 CATATG CA^TATG NspI 1296 GCATGC [GA]CATG^[CT] PaeR7I 1985 CTCGAG C^TCGAG PflFI 575 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1741 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1366 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 710 GAGCTC GAGCT^C SapI 1341 GAAGAGC GCTCTTC(1/4) SexAI 1332 ACCAGGT A^CC[AT]GGT SmlI 1985 CTCGAG C^T[CT][GA]AG SphI 1296 GCATGC GCATG^C SspI 2308 AATATT AAT^ATT Tth111I 575 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1741 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1737 CCATCCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1985 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 315 GTCTAC GT^[AC][GT]AC 3045 GTCTAC AocI 128 CCTCAGG CC^T[ACGT]AGG 2331 CCTGAGG Asp700I 906 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2813 GAACCTTTTC BanI 204 GGTGCC G^G[CT][GA]CC 2796 GGTGCC BbsI 425 GAAGAC GAAGAC(2/6) 662 GAAGAC BbvCI 686 GCTGAGG CCTCAGC(-5/-2) 1657 GCTGAGG BpuAI 425 GAAGAC GAAGAC(2/6) 662 GAAGAC Bsp1407I 1590 TGTACA T^GTACA 2314 TGTACA BsrGI 1590 TGTACA T^GTACA 2314 TGTACA BstAPI 27 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1249 GCAAGCCATGC BstYI 552 GGATCT [GA]^GATC[CT] 2336 GGATCC Bsu36I 128 CCTCAGG CC^T[ACGT]AGG 2331 CCTGAGG DraI 2241 TTTAAA TTT^AAA 2445 TTTAAA Eco81I 128 CCTCAGG CC^T[ACGT]AGG 2331 CCTGAGG EcoRI 379 GAATTC G^AATTC 1357 GAATTC MflI 552 GGATCT [GA]^GATC[CT] 2336 GGATCC PpuMI 651 AGGACCC [GA]G^G[AT]CC[CT] 1674 GGGACCT PstI 1824 CTGCAG CTGCA^G 2960 CTGCAG SspBI 1590 TGTACA T^GTACA 2314 TGTACA XhoII 552 GGATCT [GA]^GATC[CT] 2336 GGATCC XmnI 906 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2813 GAACCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI