##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AgeI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AviII       AvrII       BbeI        
BbrPI       BcgI        BlnI        BsaAI       BsaBI       
BsgI        BsiWI       BsmI        BspDI       BsrBI       
BsrFI       BssHII      Bst1107I    BstBI       BstUI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eco47III    EcoNI       EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       2378  TCCGGA               T^CCGGA
AcsI           49  GAATTC               [GA]^AATT[CT]
AcyI          619  GACGCC               G[GA]^CG[CT]C
AhdI          491  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        580  GTGCAC               G^TGCAC
AocI          108  CCTGAGG              CC^T[ACGT]AGG
ApaLI         580  GTGCAC               G^TGCAC
ApoI           49  GAATTC               [GA]^AATT[CT]
AspEI         491  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1712  TGGCCA               TGG^CCA
BamHI        1857  GGATCC               G^GATCC
BanI          126  GGTGCC               G^G[CT][GA]CC
BbvCI         471  GCTGAGG              CCTCAGC(-5/-2)
BclI         2281  TGATCA               T^GATCA
BglI         2022  GCCTCCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         160  AGATCT               A^GATCT
BlpI         1727  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1727  GCTCAGC              GC^T[ACGT]AGC
BsaHI         619  GACGCC               G[GA]^CG[CT]C
BsaWI        2378  TCCGGA               [AT]^CCGG[AT]
BseAI        2378  TCCGGA               T^CCGGA
BsiEI          72  CGGCCG               CG[GA][CT]^CG
BsmBI         486  GAGACG               CGTCTC(1/5)
Bsp1407I      599  TGTACA               T^GTACA
BspEI        2378  TCCGGA               T^CCGGA
BspHI        1269  TCATGA               T^CATGA
BsrGI         599  TGTACA               T^GTACA
BstAPI       1035  GCAGGAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        232  GGTTACC              G^GT[ACGT]ACC
BstPI         232  GGTTACC              G^GT[ACGT]ACC
BstXI        1208  CCAGTGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        108  CCTGAGG              CC^T[ACGT]AGG
CelII        1727  GCTCAGC              GC^T[ACGT]AGC
EagI           72  CGGCCG               C^GGCCG
Eam1105I      491  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          72  CGGCCG               C^GGCCG
Eco52I         72  CGGCCG               C^GGCCG
Eco81I        108  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       232  GGTTACC              G^GT[ACGT]ACC
EcoRI          49  GAATTC               G^AATTC
FbaI         2281  TGATCA               T^GATCA
Hin1I         619  GACGCC               G[GA]^CG[CT]C
MaeII         829  ACGT                 A^CGT
MluNI        1712  TGGCCA               TGG^CCA
MroI         2378  TCCGGA               T^CCGGA
MscI         1712  TGGCCA               TGG^CCA
NheI          946  GCTAGC               G^CTAGC
RcaI         1269  TCATGA               T^CATGA
SspBI         599  TGTACA               T^GTACA
StuI         1376  AGGCCT               AGG^CCT
XcmI         1757  CCAGGGCCAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflII         156  CTTAAG               C^TTAAG
             2707  CTTAAG              
AflIII        447  ACATGT               A^C[GA][CT]GT
             2170  ACATGT              
ApaI          588  GGGCCC               GGGCC^C
             2589  GGGCCC              
AspI         1741  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1873  GACTGTGTC           
BbsI         1879  GTCTTC               GAAGAC(2/6)
             2427  GTCTTC              
BciVI        1168  GTATCC               GTATCC(6/5)
             1665  GTATCC              
BfrI          156  CTTAAG               C^TTAAG
             2707  CTTAAG              
BpuAI        1879  GTCTTC               GAAGAC(2/6)
             2427  GTCTTC              
BspLU11I      447  ACATGT               A^CATGT
             2170  ACATGT              
BspMI         201  ACCTGC               ACCTGC(4/8)
             1597  ACCTGC              
BstYI         160  AGATCT               [GA]^GATC[CT]
             1857  GGATCC              
HindIII        16  AAGCTT               A^AGCTT
             1935  AAGCTT              
MflI          160  AGATCT               [GA]^GATC[CT]
             1857  GGATCC              
MslI          214  CATAATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              543  CATTACTGTG          
PaeR7I         80  CTCGAG               C^TCGAG
              695  CTCGAG              
PflFI        1741  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1873  GACTGTGTC           
PstI          353  CTGCAG               CTGCA^G
              963  CTGCAG              
SexAI         236  ACCAGGT              A^CC[AT]GGT
             2422  ACCAGGT             
TaqI           81  TCGA                 T^CGA
              696  TCGA                
Tth111I      1741  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1873  GACTGTGTC           
TthHB8I        81  TCGA                 T^CGA
              696  TCGA                
XhoI           80  CTCGAG               C^TCGAG
              695  CTCGAG              
XhoII         160  AGATCT               [GA]^GATC[CT]
             1857  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I