################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AccII AclI AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BbrPI BcgI BlnI BsaAI BsaBI BsgI BsiWI BsmI BspDI BsrBI BsrFI BssHII Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI Eco47III EcoNI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 2378 TCCGGA T^CCGGA AcsI 49 GAATTC [GA]^AATT[CT] AcyI 619 GACGCC G[GA]^CG[CT]C AhdI 491 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 580 GTGCAC G^TGCAC AocI 108 CCTGAGG CC^T[ACGT]AGG ApaLI 580 GTGCAC G^TGCAC ApoI 49 GAATTC [GA]^AATT[CT] AspEI 491 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1712 TGGCCA TGG^CCA BamHI 1857 GGATCC G^GATCC BanI 126 GGTGCC G^G[CT][GA]CC BbvCI 471 GCTGAGG CCTCAGC(-5/-2) BclI 2281 TGATCA T^GATCA BglI 2022 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 160 AGATCT A^GATCT BlpI 1727 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1727 GCTCAGC GC^T[ACGT]AGC BsaHI 619 GACGCC G[GA]^CG[CT]C BsaWI 2378 TCCGGA [AT]^CCGG[AT] BseAI 2378 TCCGGA T^CCGGA BsiEI 72 CGGCCG CG[GA][CT]^CG BsmBI 486 GAGACG CGTCTC(1/5) Bsp1407I 599 TGTACA T^GTACA BspEI 2378 TCCGGA T^CCGGA BspHI 1269 TCATGA T^CATGA BsrGI 599 TGTACA T^GTACA BstAPI 1035 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 232 GGTTACC G^GT[ACGT]ACC BstPI 232 GGTTACC G^GT[ACGT]ACC BstXI 1208 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 108 CCTGAGG CC^T[ACGT]AGG CelII 1727 GCTCAGC GC^T[ACGT]AGC EagI 72 CGGCCG C^GGCCG Eam1105I 491 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 72 CGGCCG C^GGCCG Eco52I 72 CGGCCG C^GGCCG Eco81I 108 CCTGAGG CC^T[ACGT]AGG EcoO65I 232 GGTTACC G^GT[ACGT]ACC EcoRI 49 GAATTC G^AATTC FbaI 2281 TGATCA T^GATCA Hin1I 619 GACGCC G[GA]^CG[CT]C MaeII 829 ACGT A^CGT MluNI 1712 TGGCCA TGG^CCA MroI 2378 TCCGGA T^CCGGA MscI 1712 TGGCCA TGG^CCA NheI 946 GCTAGC G^CTAGC RcaI 1269 TCATGA T^CATGA SspBI 599 TGTACA T^GTACA StuI 1376 AGGCCT AGG^CCT XcmI 1757 CCAGGGCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflII 156 CTTAAG C^TTAAG 2707 CTTAAG AflIII 447 ACATGT A^C[GA][CT]GT 2170 ACATGT ApaI 588 GGGCCC GGGCC^C 2589 GGGCCC AspI 1741 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1873 GACTGTGTC BbsI 1879 GTCTTC GAAGAC(2/6) 2427 GTCTTC BciVI 1168 GTATCC GTATCC(6/5) 1665 GTATCC BfrI 156 CTTAAG C^TTAAG 2707 CTTAAG BpuAI 1879 GTCTTC GAAGAC(2/6) 2427 GTCTTC BspLU11I 447 ACATGT A^CATGT 2170 ACATGT BspMI 201 ACCTGC ACCTGC(4/8) 1597 ACCTGC BstYI 160 AGATCT [GA]^GATC[CT] 1857 GGATCC HindIII 16 AAGCTT A^AGCTT 1935 AAGCTT MflI 160 AGATCT [GA]^GATC[CT] 1857 GGATCC MslI 214 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 543 CATTACTGTG PaeR7I 80 CTCGAG C^TCGAG 695 CTCGAG PflFI 1741 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1873 GACTGTGTC PstI 353 CTGCAG CTGCA^G 963 CTGCAG SexAI 236 ACCAGGT A^CC[AT]GGT 2422 ACCAGGT TaqI 81 TCGA T^CGA 696 TCGA Tth111I 1741 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1873 GACTGTGTC TthHB8I 81 TCGA T^CGA 696 TCGA XhoI 80 CTCGAG C^TCGAG 695 CTCGAG XhoII 160 AGATCT [GA]^GATC[CT] 1857 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BssSI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I