##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BbeI
BbrPI BcgI BlnI BsaAI BsaBI
BsgI BsiWI BsmI BspDI BsrBI
BsrFI BssHII Bst1107I BstBI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI Eco47III EcoNI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 2378 TCCGGA T^CCGGA
AcsI 49 GAATTC [GA]^AATT[CT]
AcyI 619 GACGCC G[GA]^CG[CT]C
AhdI 491 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 580 GTGCAC G^TGCAC
AocI 108 CCTGAGG CC^T[ACGT]AGG
ApaLI 580 GTGCAC G^TGCAC
ApoI 49 GAATTC [GA]^AATT[CT]
AspEI 491 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1712 TGGCCA TGG^CCA
BamHI 1857 GGATCC G^GATCC
BanI 126 GGTGCC G^G[CT][GA]CC
BbvCI 471 GCTGAGG CCTCAGC(-5/-2)
BclI 2281 TGATCA T^GATCA
BglI 2022 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 160 AGATCT A^GATCT
BlpI 1727 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1727 GCTCAGC GC^T[ACGT]AGC
BsaHI 619 GACGCC G[GA]^CG[CT]C
BsaWI 2378 TCCGGA [AT]^CCGG[AT]
BseAI 2378 TCCGGA T^CCGGA
BsiEI 72 CGGCCG CG[GA][CT]^CG
BsmBI 486 GAGACG CGTCTC(1/5)
Bsp1407I 599 TGTACA T^GTACA
BspEI 2378 TCCGGA T^CCGGA
BspHI 1269 TCATGA T^CATGA
BsrGI 599 TGTACA T^GTACA
BstAPI 1035 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 232 GGTTACC G^GT[ACGT]ACC
BstPI 232 GGTTACC G^GT[ACGT]ACC
BstXI 1208 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 108 CCTGAGG CC^T[ACGT]AGG
CelII 1727 GCTCAGC GC^T[ACGT]AGC
EagI 72 CGGCCG C^GGCCG
Eam1105I 491 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 72 CGGCCG C^GGCCG
Eco52I 72 CGGCCG C^GGCCG
Eco81I 108 CCTGAGG CC^T[ACGT]AGG
EcoO65I 232 GGTTACC G^GT[ACGT]ACC
EcoRI 49 GAATTC G^AATTC
FbaI 2281 TGATCA T^GATCA
Hin1I 619 GACGCC G[GA]^CG[CT]C
MaeII 829 ACGT A^CGT
MluNI 1712 TGGCCA TGG^CCA
MroI 2378 TCCGGA T^CCGGA
MscI 1712 TGGCCA TGG^CCA
NheI 946 GCTAGC G^CTAGC
RcaI 1269 TCATGA T^CATGA
SspBI 599 TGTACA T^GTACA
StuI 1376 AGGCCT AGG^CCT
XcmI 1757 CCAGGGCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 156 CTTAAG C^TTAAG
2707 CTTAAG
AflIII 447 ACATGT A^C[GA][CT]GT
2170 ACATGT
ApaI 588 GGGCCC GGGCC^C
2589 GGGCCC
AspI 1741 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1873 GACTGTGTC
BbsI 1879 GTCTTC GAAGAC(2/6)
2427 GTCTTC
BciVI 1168 GTATCC GTATCC(6/5)
1665 GTATCC
BfrI 156 CTTAAG C^TTAAG
2707 CTTAAG
BpuAI 1879 GTCTTC GAAGAC(2/6)
2427 GTCTTC
BspLU11I 447 ACATGT A^CATGT
2170 ACATGT
BspMI 201 ACCTGC ACCTGC(4/8)
1597 ACCTGC
BstYI 160 AGATCT [GA]^GATC[CT]
1857 GGATCC
HindIII 16 AAGCTT A^AGCTT
1935 AAGCTT
MflI 160 AGATCT [GA]^GATC[CT]
1857 GGATCC
MslI 214 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
543 CATTACTGTG
PaeR7I 80 CTCGAG C^TCGAG
695 CTCGAG
PflFI 1741 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1873 GACTGTGTC
PstI 353 CTGCAG CTGCA^G
963 CTGCAG
SexAI 236 ACCAGGT A^CC[AT]GGT
2422 ACCAGGT
TaqI 81 TCGA T^CGA
696 TCGA
Tth111I 1741 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1873 GACTGTGTC
TthHB8I 81 TCGA T^CGA
696 TCGA
XhoI 80 CTCGAG C^TCGAG
695 CTCGAG
XhoII 160 AGATCT [GA]^GATC[CT]
1857 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BssSI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I