##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI Asp718I AspEI AspI AvrII
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BstBI
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MamI MluI MroI NdeI NheI
NotI NsiI NspV PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1810 GTATAC GT^[AC][GT]AC
AocI 1859 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1878 AGCGCT AGC^GCT
AseI 2852 ATTAAT AT^TAAT
AsnI 2852 ATTAAT AT^TAAT
Asp700I 2219 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 225 GGATCC G^GATCC
BcgI 449 GCATGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2011 CTAG C^TAG
BsaAI 1819 CACGTA [CT]AC^GT[GA]
BsmBI 1125 GAGACG CGTCTC(1/5)
BsrBI 572 GAGCGG CCGCTC(-3/-3)
BsrFI 1461 GCCGGC [GA]^CCGG[CT]
BssSI 703 CTCGTG CACGAG(-5/-1)
Bst1107I 1810 GTATAC GTA^TAC
BstEII 288 GGTCACC G^GT[ACGT]ACC
BstPI 288 GGTCACC G^GT[ACGT]ACC
BstZ17I 1810 GTATAC GTA^TAC
Bsu36I 1859 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1461 GCCGGC [GA]^CCGG[CT]
Eco47III 1878 AGCGCT AGC^GCT
Eco81I 1859 CCTGAGG CC^T[ACGT]AGG
EcoO65I 288 GGTCACC G^GT[ACGT]ACC
EcoRV 2865 GATATC GAT^ATC
HincII 2449 GTTGAC GT[CT]^[GA]AC
HindII 2449 GTTGAC GT[CT]^[GA]AC
MaeI 2011 CTAG C^TAG
MfeI 421 CAATTG C^AATTG
MunI 421 CAATTG C^AATTG
NaeI 1461 GCCGGC GCC^GGC
NgoMIV 1461 GCCGGC G^CCGGC
NruI 674 TCGCGA TCG^CGA
NspI 134 GCATGC [GA]CATG^[CT]
PacI 2853 TTAATTAA TTAAT^TAA
PaeR7I 389 CTCGAG C^TCGAG
PflMI 609 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2852 ATTAAT AT^TAAT
SapI 61 GCTCTTC GCTCTTC(1/4)
SexAI 1429 ACCAGGT A^CC[AT]GGT
SphI 134 GCATGC GCATG^C
TaqI 390 TCGA T^CGA
TthHB8I 390 TCGA T^CGA
Van91I 609 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 389 CTCGAG C^TCGAG
XmnI 2219 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1733 GAATTC [GA]^AATT[CT]
2335 GAATTC
AlwNI 352 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2637 CAGAGTCTG
ApaI 1021 GGGCCC GGGCC^C
1386 GGGCCC
ApoI 1733 GAATTC [GA]^AATT[CT]
2335 GAATTC
AviII 36 TGCGCA TGC^GCA
969 TGCGCA
BalI 1228 TGGCCA TGG^CCA
2257 TGGCCA
BbeI 12 GGCGCC GGCGC^C
526 GGCGCC
BbsI 200 GAAGAC GAAGAC(2/6)
2615 GAAGAC
BlpI 1209 GCTCAGC GC^T[ACGT]AGC
2109 GCTCAGC
Bpu1102I 1209 GCTCAGC GC^T[ACGT]AGC
2109 GCTCAGC
BpuAI 200 GAAGAC GAAGAC(2/6)
2615 GAAGAC
BsaWI 929 ACCGGA [AT]^CCGG[AT]
2522 ACCGGA
BstAPI 699 GCAACTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
871 GCACGGTCTGC
CelII 1209 GCTCAGC GC^T[ACGT]AGC
2109 GCTCAGC
EaeI 1228 TGGCCA [CT]^GGCC[GA]
2257 TGGCCA
EarI 62 CTCTTC CTCTTC(1/4)
1945 GAAGAG
EcoNI 952 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1930 CCTTCCCGAGG
EcoRI 1733 GAATTC G^AATTC
2335 GAATTC
FspI 36 TGCGCA TGC^GCA
969 TGCGCA
KasI 12 GGCGCC G^GCGCC
526 GGCGCC
Ksp632I 62 CTCTTC CTCTTC(1/4)
1945 GAAGAG
MluNI 1228 TGGCCA TGG^CCA
2257 TGGCCA
MscI 1228 TGGCCA TGG^CCA
2257 TGGCCA
NarI 12 GGCGCC GG^CGCC
526 GGCGCC
PvuII 1525 CAGCTG CAG^CTG
1894 CAGCTG
SfoI 12 GGCGCC GGC^GCC
526 GGCGCC
SmaI 709 CCCGGG CCC^GGG
1041 CCCGGG
XcmI 155 CCATACTGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
284 CCATGGTCACCATGG
XmaCI 709 CCCGGG C^CCGGG
1041 CCCGGG
XmaI 709 CCCGGG C^CCGGG
1041 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII