##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BclI BcnI BglII BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoT22I FbaI FseI FspI
HapII HpaII KasI KspI MfeI
MluI MroI MspI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 521 GGTACC G^GTACC
AcyI 1643 GGCGTC G[GA]^CG[CT]C
AflII 466 CTTAAG C^TTAAG
AhdI 1942 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3029 CCTAAGG CC^T[ACGT]AGG
Asp718I 521 GGTACC G^GTACC
AspEI 1942 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3078 GAGCTC G[AT]GC[AT]^C
BciVI 3959 GTATCC GTATCC(6/5)
BfrI 466 CTTAAG C^TTAAG
BglI 2979 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 429 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1643 GGCGTC G[GA]^CG[CT]C
BsiEI 2171 CGGTCG CG[GA][CT]^CG
BsiHKAI 3078 GAGCTC G[AT]GC[AT]^C
BsmBI 1645 CGTCTC CGTCTC(1/5)
BsrDI 669 GCAATG GCAATG(2/0)
BstAPI 1173 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3029 CCTAAGG CC^T[ACGT]AGG
DraIII 1376 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1277 GACTTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1942 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3029 CCTAAGG CC^T[ACGT]AGG
EcoRI 1263 GAATTC G^AATTC
Hin1I 1643 GGCGTC G[GA]^CG[CT]C
HincII 3686 GTTAAC GT[CT]^[GA]AC
HindII 3686 GTTAAC GT[CT]^[GA]AC
HpaI 3686 GTTAAC GTT^AAC
KpnI 521 GGTACC GGTAC^C
MamI 429 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1272 CCATGG C^CATGG
PflMI 1980 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 3078 GAGCTC GAGCT^C
SbfI 3062 CCTGCAGG CCTGCA^GG
Sse8387I 3062 CCTGCAGG CCTGCA^GG
Van91I 1980 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 1676 CTCGGG C^[CT]CG[GA]G
2752 CCCGAG
BalI 36 TGGCCA TGG^CCA
1575 TGGCCA
BamHI 327 GGATCC G^GATCC
1701 GGATCC
BsmI 4279 GAATGC GAATGC(1/-1)
3678 GCATTC
BsoBI 1676 CTCGGG C^[CT]CG[GA]G
2752 CCCGAG
Bsp1407I 1907 TGTACA T^GTACA
2567 TGTACA
BspMI 1943 ACCTGC ACCTGC(4/8)
3065 GCAGGT
BsrBI 1094 CCGCTC CCGCTC(-3/-3)
3155 CCGCTC
BsrGI 1907 TGTACA T^GTACA
2567 TGTACA
BstXI 548 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1662 CCAGCAGTTTGG
BstYI 327 GGATCC [GA]^GATC[CT]
1701 GGATCC
EcoNI 408 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2383 CCTGAAGAAGG
EcoRV 429 GATATC GAT^ATC
1059 GATATC
FauI 1182 GCGGG CCCGC(4/6)
2107 GCGGG
HaeII 48 AGCGCC [GA]GCGC^[CT]
2788 AGCGCC
MaeII 100 ACGT A^CGT
1580 ACGT
MflI 327 GGATCC [GA]^GATC[CT]
1701 GGATCC
MluNI 36 TGGCCA TGG^CCA
1575 TGGCCA
MscI 36 TGGCCA TGG^CCA
1575 TGGCCA
NspI 2030 GCATGT [GA]CATG^[CT]
3865 GCATGT
SspBI 1907 TGTACA T^GTACA
2567 TGTACA
SspI 3482 AATATT AAT^ATT
3769 AATATT
StuI 2592 AGGCCT AGG^CCT
3648 AGGCCT
XhoII 327 GGATCC [GA]^GATC[CT]
1701 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HhaI
HinP1I HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XmnI