################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccI AccIII AclI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BcnI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FbaI FseI FspI HapII HpaII KasI KspI MfeI MluI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 521 GGTACC G^GTACC AcyI 1643 GGCGTC G[GA]^CG[CT]C AflII 466 CTTAAG C^TTAAG AhdI 1942 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3029 CCTAAGG CC^T[ACGT]AGG Asp718I 521 GGTACC G^GTACC AspEI 1942 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 3078 GAGCTC G[AT]GC[AT]^C BciVI 3959 GTATCC GTATCC(6/5) BfrI 466 CTTAAG C^TTAAG BglI 2979 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 429 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1643 GGCGTC G[GA]^CG[CT]C BsiEI 2171 CGGTCG CG[GA][CT]^CG BsiHKAI 3078 GAGCTC G[AT]GC[AT]^C BsmBI 1645 CGTCTC CGTCTC(1/5) BsrDI 669 GCAATG GCAATG(2/0) BstAPI 1173 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3029 CCTAAGG CC^T[ACGT]AGG DraIII 1376 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1277 GACTTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1942 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3029 CCTAAGG CC^T[ACGT]AGG EcoRI 1263 GAATTC G^AATTC Hin1I 1643 GGCGTC G[GA]^CG[CT]C HincII 3686 GTTAAC GT[CT]^[GA]AC HindII 3686 GTTAAC GT[CT]^[GA]AC HpaI 3686 GTTAAC GTT^AAC KpnI 521 GGTACC GGTAC^C MamI 429 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1272 CCATGG C^CATGG PflMI 1980 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 3078 GAGCTC GAGCT^C SbfI 3062 CCTGCAGG CCTGCA^GG Sse8387I 3062 CCTGCAGG CCTGCA^GG Van91I 1980 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaI 1676 CTCGGG C^[CT]CG[GA]G 2752 CCCGAG BalI 36 TGGCCA TGG^CCA 1575 TGGCCA BamHI 327 GGATCC G^GATCC 1701 GGATCC BsmI 4279 GAATGC GAATGC(1/-1) 3678 GCATTC BsoBI 1676 CTCGGG C^[CT]CG[GA]G 2752 CCCGAG Bsp1407I 1907 TGTACA T^GTACA 2567 TGTACA BspMI 1943 ACCTGC ACCTGC(4/8) 3065 GCAGGT BsrBI 1094 CCGCTC CCGCTC(-3/-3) 3155 CCGCTC BsrGI 1907 TGTACA T^GTACA 2567 TGTACA BstXI 548 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1662 CCAGCAGTTTGG BstYI 327 GGATCC [GA]^GATC[CT] 1701 GGATCC EcoNI 408 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2383 CCTGAAGAAGG EcoRV 429 GATATC GAT^ATC 1059 GATATC FauI 1182 GCGGG CCCGC(4/6) 2107 GCGGG HaeII 48 AGCGCC [GA]GCGC^[CT] 2788 AGCGCC MaeII 100 ACGT A^CGT 1580 ACGT MflI 327 GGATCC [GA]^GATC[CT] 1701 GGATCC MluNI 36 TGGCCA TGG^CCA 1575 TGGCCA MscI 36 TGGCCA TGG^CCA 1575 TGGCCA NspI 2030 GCATGT [GA]CATG^[CT] 3865 GCATGT SspBI 1907 TGTACA T^GTACA 2567 TGTACA SspI 3482 AATATT AAT^ATT 3769 AATATT StuI 2592 AGGCCT AGG^CCT 3648 AGGCCT XhoII 327 GGATCC [GA]^GATC[CT] 1701 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HhaI HinP1I HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI