##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BbsI BcgI BciVI BclI BfrI
BlnI BpuAI BsiWI BsmBI BspDI
BspHI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KspI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI Van91I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1605 GGTACC G^GTACC
AccIII 339 TCCGGA T^CCGGA
AcyI 526 GGCGCC G[GA]^CG[CT]C
AflIII 2390 ACATGT A^C[GA][CT]GT
Alw44I 65 GTGCAC G^TGCAC
ApaLI 65 GTGCAC G^TGCAC
Asp718I 1605 GGTACC G^GTACC
BbeI 526 GGCGCC GGCGC^C
BbvCI 2462 GCTGAGG CCTCAGC(-5/-2)
BlpI 1619 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1619 GCTGAGC GC^T[ACGT]AGC
BsaBI 475 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 526 GGCGCC G[GA]^CG[CT]C
BsaWI 339 TCCGGA [AT]^CCGG[AT]
BseAI 339 TCCGGA T^CCGGA
BsiEI 2158 CGGCCG CG[GA][CT]^CG
Bsp1407I 2599 TGTACA T^GTACA
BspEI 339 TCCGGA T^CCGGA
BspLU11I 2390 ACATGT A^CATGT
BsrGI 2599 TGTACA T^GTACA
CelII 1619 GCTGAGC GC^T[ACGT]AGC
DrdI 2525 GACCAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2158 CGGCCG C^GGCCG
EclXI 2158 CGGCCG C^GGCCG
Eco52I 2158 CGGCCG C^GGCCG
FseI 158 GGCCGGCC GGCCGG^CC
HgaI 1206 GCGTC GACGC(5/10)
Hin1I 526 GGCGCC G[GA]^CG[CT]C
KasI 526 GGCGCC G^GCGCC
KpnI 1605 GGTACC GGTAC^C
MamI 475 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 339 TCCGGA T^CCGGA
MseI 2624 TTAA T^TAA
NaeI 159 GCCGGC GCC^GGC
NarI 526 GGCGCC GG^CGCC
NcoI 54 CCATGG C^CATGG
NgoMIV 159 GCCGGC G^CCGGC
NspI 2390 ACATGT [GA]CATG^[CT]
PaeR7I 555 CTCGAG C^TCGAG
SbfI 1543 CCTGCAGG CCTGCA^GG
SfoI 526 GGCGCC GGC^GCC
SmlI 555 CTCGAG C^T[CT][GA]AG
Sse8387I 1543 CCTGCAGG CCTGCA^GG
SspBI 2599 TGTACA T^GTACA
TaqI 556 TCGA T^CGA
Tru9I 2624 TTAA T^TAA
TthHB8I 556 TCGA T^CGA
XcmI 862 CCATTCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 555 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 721 AAATTC [GA]^AATT[CT]
2591 AAATTT
ApoI 721 AAATTC [GA]^AATT[CT]
2591 AAATTT
AspI 343 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2525 GACCAAGTC
BamHI 1675 GGATCC G^GATCC
2114 GGATCC
BbrPI 96 CACGTG CAC^GTG
2397 CACGTG
BfaI 2068 CTAG C^TAG
2541 CTAG
BglII 1407 AGATCT A^GATCT
2064 AGATCT
BsaAI 96 CACGTG [CT]AC^GT[GA]
2397 CACGTG
BsgI 1176 GTGCAG GTGCAG(16/14)
1739 GTGCAG
BsmI 1127 GCATTC GAATGC(1/-1)
2165 GCATTC
BsrDI 128 GCAATG GCAATG(2/0)
2499 GCAATG
DraIII 666 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1114 CACAGTGTG
MaeI 2068 CTAG C^TAG
2541 CTAG
MaeII 97 ACGT A^CGT
2398 ACGT
PflFI 343 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2525 GACCAAGTC
PmaCI 96 CACGTG CAC^GTG
2397 CACGTG
PmlI 96 CACGTG CAC^GTG
2397 CACGTG
PpuMI 175 GGGACCT [GA]G^G[AT]CC[CT]
843 GGGTCCC
SacI 1560 GAGCTC GAGCT^C
2440 GAGCTC
StuI 1023 AGGCCT AGG^CCT
1581 AGGCCT
TfiI 1436 GAATC G^A[AT]TC
2200 GAATC
Tth111I 343 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2525 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TseI
Tsp45I Tsp509I TspRI XhoII XmaCI
XmaI