################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I AspEI AviII AvrII BbsI BcgI BciVI BclI BfrI BlnI BpuAI BsiWI BsmBI BspDI BspHI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1605 GGTACC G^GTACC AccIII 339 TCCGGA T^CCGGA AcyI 526 GGCGCC G[GA]^CG[CT]C AflIII 2390 ACATGT A^C[GA][CT]GT Alw44I 65 GTGCAC G^TGCAC ApaLI 65 GTGCAC G^TGCAC Asp718I 1605 GGTACC G^GTACC BbeI 526 GGCGCC GGCGC^C BbvCI 2462 GCTGAGG CCTCAGC(-5/-2) BlpI 1619 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1619 GCTGAGC GC^T[ACGT]AGC BsaBI 475 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 526 GGCGCC G[GA]^CG[CT]C BsaWI 339 TCCGGA [AT]^CCGG[AT] BseAI 339 TCCGGA T^CCGGA BsiEI 2158 CGGCCG CG[GA][CT]^CG Bsp1407I 2599 TGTACA T^GTACA BspEI 339 TCCGGA T^CCGGA BspLU11I 2390 ACATGT A^CATGT BsrGI 2599 TGTACA T^GTACA CelII 1619 GCTGAGC GC^T[ACGT]AGC DrdI 2525 GACCAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2158 CGGCCG C^GGCCG EclXI 2158 CGGCCG C^GGCCG Eco52I 2158 CGGCCG C^GGCCG FseI 158 GGCCGGCC GGCCGG^CC HgaI 1206 GCGTC GACGC(5/10) Hin1I 526 GGCGCC G[GA]^CG[CT]C KasI 526 GGCGCC G^GCGCC KpnI 1605 GGTACC GGTAC^C MamI 475 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 339 TCCGGA T^CCGGA MseI 2624 TTAA T^TAA NaeI 159 GCCGGC GCC^GGC NarI 526 GGCGCC GG^CGCC NcoI 54 CCATGG C^CATGG NgoMIV 159 GCCGGC G^CCGGC NspI 2390 ACATGT [GA]CATG^[CT] PaeR7I 555 CTCGAG C^TCGAG SbfI 1543 CCTGCAGG CCTGCA^GG SfoI 526 GGCGCC GGC^GCC SmlI 555 CTCGAG C^T[CT][GA]AG Sse8387I 1543 CCTGCAGG CCTGCA^GG SspBI 2599 TGTACA T^GTACA TaqI 556 TCGA T^CGA Tru9I 2624 TTAA T^TAA TthHB8I 556 TCGA T^CGA XcmI 862 CCATTCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 555 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 721 AAATTC [GA]^AATT[CT] 2591 AAATTT ApoI 721 AAATTC [GA]^AATT[CT] 2591 AAATTT AspI 343 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2525 GACCAAGTC BamHI 1675 GGATCC G^GATCC 2114 GGATCC BbrPI 96 CACGTG CAC^GTG 2397 CACGTG BfaI 2068 CTAG C^TAG 2541 CTAG BglII 1407 AGATCT A^GATCT 2064 AGATCT BsaAI 96 CACGTG [CT]AC^GT[GA] 2397 CACGTG BsgI 1176 GTGCAG GTGCAG(16/14) 1739 GTGCAG BsmI 1127 GCATTC GAATGC(1/-1) 2165 GCATTC BsrDI 128 GCAATG GCAATG(2/0) 2499 GCAATG DraIII 666 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1114 CACAGTGTG MaeI 2068 CTAG C^TAG 2541 CTAG MaeII 97 ACGT A^CGT 2398 ACGT PflFI 343 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2525 GACCAAGTC PmaCI 96 CACGTG CAC^GTG 2397 CACGTG PmlI 96 CACGTG CAC^GTG 2397 CACGTG PpuMI 175 GGGACCT [GA]G^G[AT]CC[CT] 843 GGGTCCC SacI 1560 GAGCTC GAGCT^C 2440 GAGCTC StuI 1023 AGGCCT AGG^CCT 1581 AGGCCT TfiI 1436 GAATC G^A[AT]TC 2200 GAATC Tth111I 343 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2525 GACCAAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TseI Tsp45I Tsp509I TspRI XhoII XmaCI XmaI