##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AhdI AscI AspEI AspI BalI
BamHI BbeI BbrPI BcgI BclI
BglII BsaBI BsaHI BseAI BsmBI
Bsp1407I BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstZ17I CpoI DraI
DraIII Eam1105I EcoRI EcoRV FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MluI
MluNI MroI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PflFI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacI SacII
SalI ScaI SexAI SfoI SgrAI
SspBI SspI SwaI TfiI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 862 GGTACC G^GTACC
AflII 743 CTTAAG C^TTAAG
AgeI 122 ACCGGT A^CCGGT
Alw44I 2351 GTGCAC G^TGCAC
Aor51HI 1482 AGCGCT AGC^GCT
ApaLI 2351 GTGCAC G^TGCAC
AseI 568 ATTAAT AT^TAAT
AsnI 568 ATTAAT AT^TAAT
Asp718I 862 GGTACC G^GTACC
AviII 942 TGCGCA TGC^GCA
AvrII 892 CCTAGG C^CTAGG
BbsI 180 GTCTTC GAAGAC(2/6)
BciVI 2724 GTATCC GTATCC(6/5)
BfrI 743 CTTAAG C^TTAAG
BlnI 892 CCTAGG C^CTAGG
BpuAI 180 GTCTTC GAAGAC(2/6)
BsaAI 3142 TACGTA [CT]AC^GT[GA]
BsiWI 3140 CGTACG C^GTACG
BsmI 658 GCATTC GAATGC(1/-1)
BspDI 2987 ATCGAT AT^CGAT
BspHI 952 TCATGA T^CATGA
BsrBI 2525 GAGCGG CCGCTC(-3/-3)
BstBI 1725 TTCGAA TT^CGAA
BstEII 2440 GGTGACC G^GT[ACGT]ACC
BstPI 2440 GGTGACC G^GT[ACGT]ACC
ClaI 2987 ATCGAT AT^CGAT
EagI 2263 CGGCCG C^GGCCG
EclXI 2263 CGGCCG C^GGCCG
Eco47III 1482 AGCGCT AGC^GCT
Eco52I 2263 CGGCCG C^GGCCG
EcoNI 1370 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2440 GGTGACC G^GT[ACGT]ACC
FspI 942 TGCGCA TGC^GCA
HgaI 2818 GCGTC GACGC(5/10)
KpnI 862 GGTACC GGTAC^C
MfeI 2151 CAATTG C^AATTG
MunI 2151 CAATTG C^AATTG
NcoI 913 CCATGG C^CATGG
NspV 1725 TTCGAA TT^CGAA
PaeR7I 1677 CTCGAG C^TCGAG
PinAI 122 ACCGGT A^CCGGT
PpuMI 2221 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 568 ATTAAT AT^TAAT
PvuI 2989 CGATCG CGAT^CG
RcaI 952 TCATGA T^CATGA
SbfI 1126 CCTGCAGG CCTGCA^GG
SfiI 39 GGCCACCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1725 TTCGAA TT^CGAA
SnaBI 3142 TACGTA TAC^GTA
SphI 1684 GCATGC GCATG^C
Sse8387I 1126 CCTGCAGG CCTGCA^GG
XhoI 1677 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 128 GTAGAC GT^[AC][GT]AC
1232 GTAGAC
AccII 10 CGCG CG^CG
73 CGCG
AcsI 1729 AAATTT [GA]^AATT[CT]
3193 AAATTT
ApoI 1729 AAATTT [GA]^AATT[CT]
3193 AAATTT
Asp700I 795 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1480 GAAGCGCTTC
AspHI 84 GTGCTC G[AT]GC[AT]^C
2351 GTGCAC
BbvCI 1809 CCTCAGC CCTCAGC(-5/-2)
2744 GCTGAGG
BglI 40 GCCACCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1781 GCCTTAGGGGC
BlpI 1105 GCTAAGC GC^T[ACGT]AGC
2480 GCTGAGC
Bpu1102I 1105 GCTAAGC GC^T[ACGT]AGC
2480 GCTGAGC
BsaI 2141 GAGACC GGTCTC(1/5)
1365 GGTCTC
BsaWI 122 ACCGGT [AT]^CCGG[AT]
2196 ACCGGA
BsiEI 2263 CGGCCG CG[GA][CT]^CG
2989 CGATCG
BsiHKAI 84 GTGCTC G[AT]GC[AT]^C
2351 GTGCAC
BsrFI 122 ACCGGT [GA]^CCGG[CT]
1308 ACCGGC
BstUI 10 CGCG CG^CG
73 CGCG
CelII 1105 GCTAAGC GC^T[ACGT]AGC
2480 GCTGAGC
Cfr10I 122 ACCGGT [GA]^CCGG[CT]
1308 ACCGGC
DrdI 644 GACACTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
749 GACTTCTGTGTC
EcoT22I 621 ATGCAT ATGCA^T
1425 ATGCAT
HaeII 1482 AGCGCT [GA]GCGC^[CT]
2547 GGCGCT
HinfI 726 GAGTC G^A[ACGT]TC
1802 GAGTC
MaeII 2896 ACGT A^CGT
3143 ACGT
MvnI 10 CGCG CG^CG
73 CGCG
NsiI 621 ATGCAT ATGCA^T
1425 ATGCAT
PleI 726 GAGTC GAGTC(4/5)
1802 GAGTC
SmaI 26 CCCGGG CCC^GGG
44 CCCGGG
SpeI 1345 ACTAGT A^CTAGT
1939 ACTAGT
XmaCI 26 CCCGGG C^CCGGG
44 CCCGGG
XmaI 26 CCCGGG C^CCGGG
44 CCCGGG
XmnI 795 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1480 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII