##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII BamHI BbeI BbrPI BclI
BfrI BsaAI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 139 GTAGAC GT^[AC][GT]AC
AcsI 1026 GAATTC [GA]^AATT[CT]
Alw44I 1410 GTGCAC G^TGCAC
ApaLI 1410 GTGCAC G^TGCAC
ApoI 1026 GAATTC [GA]^AATT[CT]
AspI 447 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1475 CCCGGG C^[CT]CG[GA]G
AvrII 1372 CCTAGG C^CTAGG
BbsI 1597 GAAGAC GAAGAC(2/6)
BbvCI 1440 CCTCAGC CCTCAGC(-5/-2)
BcgI 1215 GCATGAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 840 GGATAC GTATCC(6/5)
BglII 719 AGATCT A^GATCT
BlnI 1372 CCTAGG C^CTAGG
BlpI 825 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 825 GCTGAGC GC^T[ACGT]AGC
BpuAI 1597 GAAGAC GAAGAC(2/6)
BsaBI 2256 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2593 GAATGC GAATGC(1/-1)
BsoBI 1475 CCCGGG C^[CT]CG[GA]G
Bsp1407I 38 TGTACA T^GTACA
BsrFI 2192 GCCGGT [GA]^CCGG[CT]
BsrGI 38 TGTACA T^GTACA
CelII 825 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2192 GCCGGT [GA]^CCGG[CT]
DraIII 1948 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1099 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1026 GAATTC G^AATTC
HincII 2209 GTCAAC GT[CT]^[GA]AC
HindII 2209 GTCAAC GT[CT]^[GA]AC
MaeII 1505 ACGT A^CGT
MamI 2256 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 187 TTAA T^TAA
NcoI 2637 CCATGG C^CATGG
PflFI 447 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2322 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 1475 CCCGGG CCC^GGG
SspBI 38 TGTACA T^GTACA
Tru9I 187 TTAA T^TAA
Tsp45I 2173 GTGAC ^GT[GC]AC
Tsp509I 1027 AATT ^AATT
Tth111I 447 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2322 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1089 CCAGAGGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1475 CCCGGG C^CCGGG
XmaI 1475 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 1910 GACGCC G[GA]^CG[CT]C
2040 GGCGTC
BsaHI 1910 GACGCC G[GA]^CG[CT]C
2040 GGCGTC
BsaI 512 GAGACC GGTCTC(1/5)
2311 GAGACC
BsmBI 2074 GAGACG CGTCTC(1/5)
2087 GAGACG
BsrBI 1842 CCGCTC CCGCTC(-3/-3)
2491 GAGCGG
CfoI 606 GCGC GCG^C
2375 GCGC
DsaI 1400 CCACGG C^C[GA][CT]GG
2637 CCATGG
HhaI 606 GCGC GCG^C
2375 GCGC
Hin1I 1910 GACGCC G[GA]^CG[CT]C
2040 GGCGTC
HinP1I 606 GCGC G^CGC
2375 GCGC
MaeIII 2173 GTGAC ^GT[ACGT]AC
2649 GTAAC
NheI 990 GCTAGC G^CTAGC
2095 GCTAGC
SphI 84 GCATGC GCATG^C
1736 GCATGC
TaqI 1224 TCGA T^CGA
2015 TCGA
TthHB8I 1224 TCGA T^CGA
2015 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI Asp700I AspHI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
TseI TspRI XhoII XmnI