################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII BamHI BbeI BbrPI BclI BfrI BsaAI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 139 GTAGAC GT^[AC][GT]AC AcsI 1026 GAATTC [GA]^AATT[CT] Alw44I 1410 GTGCAC G^TGCAC ApaLI 1410 GTGCAC G^TGCAC ApoI 1026 GAATTC [GA]^AATT[CT] AspI 447 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1475 CCCGGG C^[CT]CG[GA]G AvrII 1372 CCTAGG C^CTAGG BbsI 1597 GAAGAC GAAGAC(2/6) BbvCI 1440 CCTCAGC CCTCAGC(-5/-2) BcgI 1215 GCATGAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 840 GGATAC GTATCC(6/5) BglII 719 AGATCT A^GATCT BlnI 1372 CCTAGG C^CTAGG BlpI 825 GCTGAGC GC^T[ACGT]AGC Bpu1102I 825 GCTGAGC GC^T[ACGT]AGC BpuAI 1597 GAAGAC GAAGAC(2/6) BsaBI 2256 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 2593 GAATGC GAATGC(1/-1) BsoBI 1475 CCCGGG C^[CT]CG[GA]G Bsp1407I 38 TGTACA T^GTACA BsrFI 2192 GCCGGT [GA]^CCGG[CT] BsrGI 38 TGTACA T^GTACA CelII 825 GCTGAGC GC^T[ACGT]AGC Cfr10I 2192 GCCGGT [GA]^CCGG[CT] DraIII 1948 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1099 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1026 GAATTC G^AATTC HincII 2209 GTCAAC GT[CT]^[GA]AC HindII 2209 GTCAAC GT[CT]^[GA]AC MaeII 1505 ACGT A^CGT MamI 2256 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 187 TTAA T^TAA NcoI 2637 CCATGG C^CATGG PflFI 447 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2322 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SmaI 1475 CCCGGG CCC^GGG SspBI 38 TGTACA T^GTACA Tru9I 187 TTAA T^TAA Tsp45I 2173 GTGAC ^GT[GC]AC Tsp509I 1027 AATT ^AATT Tth111I 447 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2322 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1089 CCAGAGGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1475 CCCGGG C^CCGGG XmaI 1475 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcyI 1910 GACGCC G[GA]^CG[CT]C 2040 GGCGTC BsaHI 1910 GACGCC G[GA]^CG[CT]C 2040 GGCGTC BsaI 512 GAGACC GGTCTC(1/5) 2311 GAGACC BsmBI 2074 GAGACG CGTCTC(1/5) 2087 GAGACG BsrBI 1842 CCGCTC CCGCTC(-3/-3) 2491 GAGCGG CfoI 606 GCGC GCG^C 2375 GCGC DsaI 1400 CCACGG C^C[GA][CT]GG 2637 CCATGG HhaI 606 GCGC GCG^C 2375 GCGC Hin1I 1910 GACGCC G[GA]^CG[CT]C 2040 GGCGTC HinP1I 606 GCGC G^CGC 2375 GCGC MaeIII 2173 GTGAC ^GT[ACGT]AC 2649 GTAAC NheI 990 GCTAGC G^CTAGC 2095 GCTAGC SphI 84 GCATGC GCATG^C 1736 GCATGC TaqI 1224 TCGA T^CGA 2015 TCGA TthHB8I 1224 TCGA T^CGA 2015 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI TseI TspRI XhoII XmnI