################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII AccII AccIII AclI AflII AflIII AgeI AhdI AscI Asp700I AspEI AviII AvrII BalI BbeI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI FspI HpaI KasI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 951 GGTACC G^GTACC AcyI 2500 GGCGTC G[GA]^CG[CT]C Alw44I 2260 GTGCAC G^TGCAC Aor51HI 3209 AGCGCT AGC^GCT ApaI 2711 GGGCCC GGGCC^C ApaLI 2260 GTGCAC G^TGCAC AseI 3404 ATTAAT AT^TAAT AsnI 3404 ATTAAT AT^TAAT Asp718I 951 GGTACC G^GTACC AspI 727 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1035 CACGTG CAC^GTG BglI 1416 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1035 CACGTG [CT]AC^GT[GA] BsaHI 2500 GGCGTC G[GA]^CG[CT]C BsaWI 1945 TCCGGT [AT]^CCGG[AT] BsrBI 1929 GAGCGG CCGCTC(-3/-3) BssSI 588 CACGAG CACGAG(-5/-1) BstEII 2246 GGTTACC G^GT[ACGT]ACC BstPI 2246 GGTTACC G^GT[ACGT]ACC Eco47III 3209 AGCGCT AGC^GCT EcoO65I 2246 GGTTACC G^GT[ACGT]ACC EcoRI 799 GAATTC G^AATTC EcoT22I 960 ATGCAT ATGCA^T Hin1I 2500 GGCGTC G[GA]^CG[CT]C HindIII 2616 AAGCTT A^AGCTT KpnI 951 GGTACC GGTAC^C NsiI 960 ATGCAT ATGCA^T PflFI 727 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1035 CACGTG CAC^GTG PmlI 1035 CACGTG CAC^GTG PpuMI 829 GGGACCT [GA]G^G[AT]CC[CT] PshBI 3404 ATTAAT AT^TAAT SalI 65 GTCGAC G^TCGAC SphI 405 GCATGC GCATG^C Tth111I 727 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BamHI 2337 GGATCC G^GATCC 2488 GGATCC BsaBI 293 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 564 GATTGCGATC BspMI 2692 ACCTGC ACCTGC(4/8) 2996 GCAGGT EaeI 1903 TGGCCG [CT]^GGCC[GA] 2291 TGGCCG HgaI 68 GACGC GACGC(5/10) 2501 GCGTC MamI 293 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 564 GATTGCGATC MslI 1035 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3223 CATACAAATG SacI 1546 GAGCTC GAGCT^C 2394 GAGCTC SapI 1548 GCTCTTC GCTCTTC(1/4) 2403 GCTCTTC SmaI 58 CCCGGG CCC^GGG 1786 CCCGGG SmlI 647 CTTGAG C^T[CT][GA]AG 2397 CTCAAG SspI 449 AATATT AAT^ATT 481 AATATT StuI 336 AGGCCT AGG^CCT 2154 AGGCCT TaqI 66 TCGA T^CGA 1544 TCGA TthHB8I 66 TCGA T^CGA 1544 TCGA XcmI 583 CCAGGCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1559 CCAGGCCCAAGCTGG XmaCI 58 CCCGGG C^CCGGG 1786 CCCGGG XmaI 58 CCCGGG C^CCGGG 1786 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII