##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI AscI Asp700I
AspEI AviII AvrII BalI BbeI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoRV FbaI FseI
FspI HpaI KasI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 951 GGTACC G^GTACC
AcyI 2500 GGCGTC G[GA]^CG[CT]C
Alw44I 2260 GTGCAC G^TGCAC
Aor51HI 3209 AGCGCT AGC^GCT
ApaI 2711 GGGCCC GGGCC^C
ApaLI 2260 GTGCAC G^TGCAC
AseI 3404 ATTAAT AT^TAAT
AsnI 3404 ATTAAT AT^TAAT
Asp718I 951 GGTACC G^GTACC
AspI 727 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1035 CACGTG CAC^GTG
BglI 1416 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1035 CACGTG [CT]AC^GT[GA]
BsaHI 2500 GGCGTC G[GA]^CG[CT]C
BsaWI 1945 TCCGGT [AT]^CCGG[AT]
BsrBI 1929 GAGCGG CCGCTC(-3/-3)
BssSI 588 CACGAG CACGAG(-5/-1)
BstEII 2246 GGTTACC G^GT[ACGT]ACC
BstPI 2246 GGTTACC G^GT[ACGT]ACC
Eco47III 3209 AGCGCT AGC^GCT
EcoO65I 2246 GGTTACC G^GT[ACGT]ACC
EcoRI 799 GAATTC G^AATTC
EcoT22I 960 ATGCAT ATGCA^T
Hin1I 2500 GGCGTC G[GA]^CG[CT]C
HindIII 2616 AAGCTT A^AGCTT
KpnI 951 GGTACC GGTAC^C
NsiI 960 ATGCAT ATGCA^T
PflFI 727 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1035 CACGTG CAC^GTG
PmlI 1035 CACGTG CAC^GTG
PpuMI 829 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 3404 ATTAAT AT^TAAT
SalI 65 GTCGAC G^TCGAC
SphI 405 GCATGC GCATG^C
Tth111I 727 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BamHI 2337 GGATCC G^GATCC
2488 GGATCC
BsaBI 293 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
564 GATTGCGATC
BspMI 2692 ACCTGC ACCTGC(4/8)
2996 GCAGGT
EaeI 1903 TGGCCG [CT]^GGCC[GA]
2291 TGGCCG
HgaI 68 GACGC GACGC(5/10)
2501 GCGTC
MamI 293 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
564 GATTGCGATC
MslI 1035 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3223 CATACAAATG
SacI 1546 GAGCTC GAGCT^C
2394 GAGCTC
SapI 1548 GCTCTTC GCTCTTC(1/4)
2403 GCTCTTC
SmaI 58 CCCGGG CCC^GGG
1786 CCCGGG
SmlI 647 CTTGAG C^T[CT][GA]AG
2397 CTCAAG
SspI 449 AATATT AAT^ATT
481 AATATT
StuI 336 AGGCCT AGG^CCT
2154 AGGCCT
TaqI 66 TCGA T^CGA
1544 TCGA
TthHB8I 66 TCGA T^CGA
1544 TCGA
XcmI 583 CCAGGCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1559 CCAGGCCCAAGCTGG
XmaCI 58 CCCGGG C^CCGGG
1786 CCCGGG
XmaI 58 CCCGGG C^CCGGG
1786 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII