##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbrPI BciVI BfrI
BlnI BsaBI BseAI BsiWI BsmI
BspDI BspEI BspLU11I BsrBI BssHII
BssSI BstAPI ClaI CpoI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI FseI FspI HaeII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MluI MluNI MroI MscI
NarI NdeI NotI NruI NspI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 2039 ACACGT A^C[GA][CT]GT
Alw44I 2601 GTGCAC G^TGCAC
ApaI 2184 GGGCCC GGGCC^C
ApaLI 2601 GTGCAC G^TGCAC
Asp700I 1504 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BglI 2719 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1149 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1149 GCTGAGC GC^T[ACGT]AGC
BsaAI 2040 CACGTA [CT]AC^GT[GA]
BsiEI 321 CGATCG CG[GA][CT]^CG
Bsp1407I 3581 TGTACA T^GTACA
BsrDI 1938 CATTGC GCAATG(2/0)
BsrFI 1840 GCCGGC [GA]^CCGG[CT]
BsrGI 3581 TGTACA T^GTACA
Bst1107I 1407 GTATAC GTA^TAC
BstBI 836 TTCGAA TT^CGAA
BstZ17I 1407 GTATAC GTA^TAC
CelII 1149 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1840 GCCGGC [GA]^CCGG[CT]
DraI 3790 TTTAAA TTT^AAA
DraIII 2272 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3216 GAATTC G^AATTC
EcoT22I 1417 ATGCAT ATGCA^T
FauI 225 CCCGC CCCGC(4/6)
MfeI 1708 CAATTG C^AATTG
MunI 1708 CAATTG C^AATTG
NaeI 1840 GCCGGC GCC^GGC
NcoI 567 CCATGG C^CATGG
NgoMIV 1840 GCCGGC G^CCGGC
NheI 126 GCTAGC G^CTAGC
NsiI 1417 ATGCAT ATGCA^T
NspV 836 TTCGAA TT^CGAA
PshAI 1164 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3240 CTGCAG CTGCA^G
PvuI 321 CGATCG CGAT^CG
SfuI 836 TTCGAA TT^CGAA
SmaI 1947 CCCGGG CCC^GGG
SspBI 3581 TGTACA T^GTACA
StuI 1616 AGGCCT AGG^CCT
XmaCI 1947 CCCGGG C^CCGGG
XmaI 1947 CCCGGG C^CCGGG
XmnI 1504 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 610 GACGCC G[GA]^CG[CT]C
2077 GGCGTC
AocI 1053 CCTCAGG CC^T[ACGT]AGG
3176 CCTGAGG
AspI 676 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
877 GACATGGTC
AvaI 1947 CCCGGG C^[CT]CG[GA]G
2802 CCCGAG
BcgI 11 CGAGCCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
498 GCACCGCAGTCG
BclI 1118 TGATCA T^GATCA
3527 TGATCA
BglII 95 AGATCT A^GATCT
2562 AGATCT
BsaHI 610 GACGCC G[GA]^CG[CT]C
2077 GGCGTC
BsgI 249 CTGCAC GTGCAG(16/14)
2556 GTGCAG
BsmBI 519 CGTCTC CGTCTC(1/5)
1424 CGTCTC
BsoBI 1947 CCCGGG C^[CT]CG[GA]G
2802 CCCGAG
BspHI 1013 TCATGA T^CATGA
3343 TCATGA
BspMI 1175 GCAGGT ACCTGC(4/8)
3886 GCAGGT
BstEII 51 GGTCACC G^GT[ACGT]ACC
2976 GGTCACC
BstPI 51 GGTCACC G^GT[ACGT]ACC
2976 GGTCACC
BstXI 1886 CCAAACGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2351 CCAAGATGGTGG
Bsu36I 1053 CCTCAGG CC^T[ACGT]AGG
3176 CCTGAGG
CfoI 1932 GCGC GCG^C
2607 GCGC
DrdI 714 GACGTACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1210 GACGAGGCTGTC
EaeI 532 CGGCCA [CT]^GGCC[GA]
2717 CGGCCA
Eco81I 1053 CCTCAGG CC^T[ACGT]AGG
3176 CCTGAGG
EcoO65I 51 GGTCACC G^GT[ACGT]ACC
2976 GGTCACC
EcoRV 2493 GATATC GAT^ATC
3407 GATATC
FbaI 1118 TGATCA T^GATCA
3527 TGATCA
HhaI 1932 GCGC GCG^C
2607 GCGC
Hin1I 610 GACGCC G[GA]^CG[CT]C
2077 GGCGTC
HinP1I 1932 GCGC G^CGC
2607 GCGC
PacI 3446 TTAATTAA TTAAT^TAA
3485 TTAATTAA
PflFI 676 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
877 GACATGGTC
PpuMI 685 AGGACCT [GA]G^G[AT]CC[CT]
2798 GGGTCCC
RcaI 1013 TCATGA T^CATGA
3343 TCATGA
SspI 425 AATATT AAT^ATT
2899 AATATT
Tth111I 676 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
877 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII