################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BciVI BfrI BlnI BsaBI BseAI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI BstAPI ClaI CpoI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI FseI FspI HaeII HindIII HpaI KasI KpnI Ksp632I KspI MamI MluI MluNI MroI MscI NarI NdeI NotI NruI NspI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 2039 ACACGT A^C[GA][CT]GT Alw44I 2601 GTGCAC G^TGCAC ApaI 2184 GGGCCC GGGCC^C ApaLI 2601 GTGCAC G^TGCAC Asp700I 1504 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BglI 2719 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1149 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1149 GCTGAGC GC^T[ACGT]AGC BsaAI 2040 CACGTA [CT]AC^GT[GA] BsiEI 321 CGATCG CG[GA][CT]^CG Bsp1407I 3581 TGTACA T^GTACA BsrDI 1938 CATTGC GCAATG(2/0) BsrFI 1840 GCCGGC [GA]^CCGG[CT] BsrGI 3581 TGTACA T^GTACA Bst1107I 1407 GTATAC GTA^TAC BstBI 836 TTCGAA TT^CGAA BstZ17I 1407 GTATAC GTA^TAC CelII 1149 GCTGAGC GC^T[ACGT]AGC Cfr10I 1840 GCCGGC [GA]^CCGG[CT] DraI 3790 TTTAAA TTT^AAA DraIII 2272 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 3216 GAATTC G^AATTC EcoT22I 1417 ATGCAT ATGCA^T FauI 225 CCCGC CCCGC(4/6) MfeI 1708 CAATTG C^AATTG MunI 1708 CAATTG C^AATTG NaeI 1840 GCCGGC GCC^GGC NcoI 567 CCATGG C^CATGG NgoMIV 1840 GCCGGC G^CCGGC NheI 126 GCTAGC G^CTAGC NsiI 1417 ATGCAT ATGCA^T NspV 836 TTCGAA TT^CGAA PshAI 1164 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 3240 CTGCAG CTGCA^G PvuI 321 CGATCG CGAT^CG SfuI 836 TTCGAA TT^CGAA SmaI 1947 CCCGGG CCC^GGG SspBI 3581 TGTACA T^GTACA StuI 1616 AGGCCT AGG^CCT XmaCI 1947 CCCGGG C^CCGGG XmaI 1947 CCCGGG C^CCGGG XmnI 1504 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 610 GACGCC G[GA]^CG[CT]C 2077 GGCGTC AocI 1053 CCTCAGG CC^T[ACGT]AGG 3176 CCTGAGG AspI 676 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 877 GACATGGTC AvaI 1947 CCCGGG C^[CT]CG[GA]G 2802 CCCGAG BcgI 11 CGAGCCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 498 GCACCGCAGTCG BclI 1118 TGATCA T^GATCA 3527 TGATCA BglII 95 AGATCT A^GATCT 2562 AGATCT BsaHI 610 GACGCC G[GA]^CG[CT]C 2077 GGCGTC BsgI 249 CTGCAC GTGCAG(16/14) 2556 GTGCAG BsmBI 519 CGTCTC CGTCTC(1/5) 1424 CGTCTC BsoBI 1947 CCCGGG C^[CT]CG[GA]G 2802 CCCGAG BspHI 1013 TCATGA T^CATGA 3343 TCATGA BspMI 1175 GCAGGT ACCTGC(4/8) 3886 GCAGGT BstEII 51 GGTCACC G^GT[ACGT]ACC 2976 GGTCACC BstPI 51 GGTCACC G^GT[ACGT]ACC 2976 GGTCACC BstXI 1886 CCAAACGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2351 CCAAGATGGTGG Bsu36I 1053 CCTCAGG CC^T[ACGT]AGG 3176 CCTGAGG CfoI 1932 GCGC GCG^C 2607 GCGC DrdI 714 GACGTACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1210 GACGAGGCTGTC EaeI 532 CGGCCA [CT]^GGCC[GA] 2717 CGGCCA Eco81I 1053 CCTCAGG CC^T[ACGT]AGG 3176 CCTGAGG EcoO65I 51 GGTCACC G^GT[ACGT]ACC 2976 GGTCACC EcoRV 2493 GATATC GAT^ATC 3407 GATATC FbaI 1118 TGATCA T^GATCA 3527 TGATCA HhaI 1932 GCGC GCG^C 2607 GCGC Hin1I 610 GACGCC G[GA]^CG[CT]C 2077 GGCGTC HinP1I 1932 GCGC G^CGC 2607 GCGC PacI 3446 TTAATTAA TTAAT^TAA 3485 TTAATTAA PflFI 676 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 877 GACATGGTC PpuMI 685 AGGACCT [GA]G^G[AT]CC[CT] 2798 GGGTCCC RcaI 1013 TCATGA T^CATGA 3343 TCATGA SspI 425 AATATT AAT^ATT 2899 AATATT Tth111I 676 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 877 GACATGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII