##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccI AclI AgeI AhdI Alw44I
ApaLI AseI AsnI Asp700I AspEI
BbrPI BcgI BclI BglII BsaAI
BsaBI BsiWI BspDI Bst1107I BstAPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoRV EcoT22I FbaI FseI HindIII
HpaI KspI MamI MfeI MunI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacI SacII SalI
SapI SbfI ScaI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 136 GACGTC GACGT^C
Acc65I 1111 GGTACC G^GTACC
AccIII 2595 TCCGGA T^CCGGA
AflII 809 CTTAAG C^TTAAG
Aor51HI 341 AGCGCT AGC^GCT
AscI 716 GGCGCGCC GG^CGCGCC
Asp718I 1111 GGTACC G^GTACC
AspHI 215 GAGCAC G[AT]GC[AT]^C
AspI 2500 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 602 TGCGCA TGC^GCA
BamHI 846 GGATCC G^GATCC
BbeI 468 GGCGCC GGCGC^C
BciVI 3030 GTATCC GTATCC(6/5)
BfrI 809 CTTAAG C^TTAAG
BlpI 361 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 361 GCTGAGC GC^T[ACGT]AGC
BseAI 2595 TCCGGA T^CCGGA
BsiHKAI 215 GAGCAC G[AT]GC[AT]^C
BspEI 2595 TCCGGA T^CCGGA
BspHI 2357 TCATGA T^CATGA
BspLU11I 1291 ACATGT A^CATGT
BsrBI 261 GAGCGG CCGCTC(-3/-3)
BstBI 3394 TTCGAA TT^CGAA
BstEII 1444 GGTGACC G^GT[ACGT]ACC
BstPI 1444 GGTGACC G^GT[ACGT]ACC
BstYI 846 GGATCC [GA]^GATC[CT]
CelII 361 GCTGAGC GC^T[ACGT]AGC
DraIII 1965 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2665 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1868 CTCTTC CTCTTC(1/4)
Eco47III 341 AGCGCT AGC^GCT
EcoO65I 1444 GGTGACC G^GT[ACGT]ACC
FspI 602 TGCGCA TGC^GCA
HincII 2013 GTCAAC GT[CT]^[GA]AC
HindII 2013 GTCAAC GT[CT]^[GA]AC
KasI 468 GGCGCC G^GCGCC
KpnI 1111 GGTACC GGTAC^C
Ksp632I 1868 CTCTTC CTCTTC(1/4)
MflI 846 GGATCC [GA]^GATC[CT]
MluI 462 ACGCGT A^CGCGT
MroI 2595 TCCGGA T^CCGGA
NarI 468 GGCGCC GG^CGCC
NdeI 1512 CATATG CA^TATG
NheI 1935 GCTAGC G^CTAGC
NotI 235 GCGGCCGC GC^GGCCGC
NspI 1291 ACATGT [GA]CATG^[CT]
NspV 3394 TTCGAA TT^CGAA
PflFI 2500 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2121 CTGCAG CTGCA^G
PvuI 2706 CGATCG CGAT^CG
RcaI 2357 TCATGA T^CATGA
SfaNI 484 GATGC GCATC(5/9)
SfoI 468 GGCGCC GGC^GCC
SfuI 3394 TTCGAA TT^CGAA
SspI 1762 AATATT AAT^ATT
Tth111I 2500 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 846 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 804 GAATTC [GA]^AATT[CT]
2519 GAATTC
AflIII 462 ACGCGT A^C[GA][CT]GT
1291 ACATGT
AlwI 847 GATCC GGATC(4/5)
846 GGATC
AlwNI 2124 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
3144 CAGGCTCTG
ApoI 804 GAATTC [GA]^AATT[CT]
2519 GAATTC
AvrII 1630 CCTAGG C^CTAGG
2481 CCTAGG
BbsI 1863 GTCTTC GAAGAC(2/6)
2629 GTCTTC
BlnI 1630 CCTAGG C^CTAGG
2481 CCTAGG
BpuAI 1863 GTCTTC GAAGAC(2/6)
2629 GTCTTC
BsaWI 377 ACCGGA [AT]^CCGG[AT]
2595 TCCGGA
BseRI 544 CTCCTC GAGGAG(10/8)
2848 CTCCTC
BsmBI 283 CGTCTC CGTCTC(1/5)
2133 GAGACG
BsmI 2379 GAATGC GAATGC(1/-1)
1645 GCATTC
Bsp1407I 1150 TGTACA T^GTACA
1656 TGTACA
BsrFI 162 GCCGGC [GA]^CCGG[CT]
995 GCCGGC
BsrGI 1150 TGTACA T^GTACA
1656 TGTACA
BssSI 1780 CACGAG CACGAG(-5/-1)
679 CTCGTG
Cfr10I 162 GCCGGC [GA]^CCGG[CT]
995 GCCGGC
EagI 164 CGGCCG C^GGCCG
236 CGGCCG
EclXI 164 CGGCCG C^GGCCG
236 CGGCCG
Eco52I 164 CGGCCG C^GGCCG
236 CGGCCG
EcoNI 1178 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2960 CCTTCAGGAGG
EcoRI 804 GAATTC G^AATTC
2519 GAATTC
MseI 810 TTAA T^TAA
2451 TTAA
NaeI 162 GCCGGC GCC^GGC
995 GCCGGC
NgoMIV 162 GCCGGC G^CCGGC
995 GCCGGC
PflMI 903 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2893 CCAACAGGTGG
SexAI 902 ACCAGGT A^CC[AT]GGT
3154 ACCTGGT
SfiI 165 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
228 GGCCGCAGCGGCC
SmaI 299 CCCGGG CCC^GGG
523 CCCGGG
SspBI 1150 TGTACA T^GTACA
1656 TGTACA
StuI 768 AGGCCT AGG^CCT
2538 AGGCCT
Tru9I 810 TTAA T^TAA
2451 TTAA
Van91I 903 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2893 CCAACAGGTGG
XmaCI 299 CCCGGG C^CCGGG
523 CCCGGG
XmaI 299 CCCGGG C^CCGGG
523 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AocI ApaI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI