##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI BamHI BciVI BclI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII EagI EclXI Eco52I
EcoO65I EcoT22I FbaI FseI HindIII
HpaI KpnI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I RsrII
SalI ScaI SfiI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 465 CTTAAG C^TTAAG
AflIII 1474 ACGTGT A^C[GA][CT]GT
AhdI 1452 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 316 AGCGCT AGC^GCT
Asp700I 3416 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1452 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2134 CCTAGG C^CTAGG
BbeI 365 GGCGCC GGCGC^C
BbrPI 3443 CACGTG CAC^GTG
BcgI 590 CGAGGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 465 CTTAAG C^TTAAG
BglI 228 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2134 CCTAGG C^CTAGG
BsaAI 3443 CACGTG [CT]AC^GT[GA]
BsaWI 1255 ACCGGA [AT]^CCGG[AT]
BsiEI 1078 CGATCG CG[GA][CT]^CG
BsmI 1267 GCATTC GAATGC(1/-1)
BspHI 1348 TCATGA T^CATGA
BssHII 44 GCGCGC G^CGCGC
BstAPI 2378 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 1452 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 316 AGCGCT AGC^GCT
EcoRI 1344 GAATTC G^AATTC
EcoRV 2512 GATATC GAT^ATC
KasI 365 GGCGCC G^GCGCC
NarI 365 GGCGCC GG^CGCC
NheI 3112 GCTAGC G^CTAGC
NspI 1693 ACATGC [GA]CATG^[CT]
PaeR7I 2361 CTCGAG C^TCGAG
PmaCI 3443 CACGTG CAC^GTG
PmlI 3443 CACGTG CAC^GTG
PvuI 1078 CGATCG CGAT^CG
RcaI 1348 TCATGA T^CATGA
SacI 2673 GAGCTC GAGCT^C
SapI 2074 GAAGAGC GCTCTTC(1/4)
SbfI 1232 CCTGCAGG CCTGCA^GG
SexAI 3406 ACCAGGT A^CC[AT]GGT
SfoI 365 GGCGCC GGC^GCC
SmaI 2049 CCCGGG CCC^GGG
Sse8387I 1232 CCTGCAGG CCTGCA^GG
StuI 744 AGGCCT AGG^CCT
XhoI 2361 CTCGAG C^TCGAG
XmaCI 2049 CCCGGG C^CCGGG
XmaI 2049 CCCGGG C^CCGGG
XmnI 3416 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AviII 75 TGCGCA TGC^GCA
925 TGCGCA
BalI 235 TGGCCA TGG^CCA
1777 TGGCCA
BglII 394 AGATCT A^GATCT
1849 AGATCT
BlpI 2014 GCTAAGC GC^T[ACGT]AGC
2054 GCTCAGC
Bpu1102I 2014 GCTAAGC GC^T[ACGT]AGC
2054 GCTCAGC
BsaBI 395 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1079 GATCGAAATC
BsmBI 3425 CGTCTC CGTCTC(1/5)
3166 GAGACG
BstBI 1123 TTCGAA TT^CGAA
1731 TTCGAA
BstXI 415 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3307 CCAGCACGCTGG
CelII 2014 GCTAAGC GC^T[ACGT]AGC
2054 GCTCAGC
DrdI 96 GACCCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1275 GACACCCGTGTC
FspI 75 TGCGCA TGC^GCA
925 TGCGCA
HincII 105 GTCAAC GT[CT]^[GA]AC
1245 GTCAAC
HindII 105 GTCAAC GT[CT]^[GA]AC
1245 GTCAAC
MamI 395 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1079 GATCGAAATC
MluNI 235 TGGCCA TGG^CCA
1777 TGGCCA
MscI 235 TGGCCA TGG^CCA
1777 TGGCCA
NspV 1123 TTCGAA TT^CGAA
1731 TTCGAA
PflMI 257 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
686 CCAGACCCTGG
PpuMI 259 AGGACCT [GA]G^G[AT]CC[CT]
760 AGGTCCC
SfuI 1123 TTCGAA TT^CGAA
1731 TTCGAA
Van91I 257 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
686 CCAGACCCTGG
XcmI 742 CCAGGCCTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3128 CCAGAAGTGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII