##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BalI BbeI BbvCI BclI
BcnI BglI BlnI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI BstEII BstPI BstXI Cfr10I
ClaI CpoI DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
KasI KpnI Ksp632I KspI MamI
MluI MluNI MroI MscI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmeI PshAI PvuI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccII 114 CGCG CG^CG
AclI 487 AACGTT AA^CGTT
AflII 2033 CTTAAG C^TTAAG
AocI 270 CCTTAGG CC^T[ACGT]AGG
AseI 1301 ATTAAT AT^TAAT
AsnI 1301 ATTAAT AT^TAAT
Asp700I 397 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 765 GAGCAC G[AT]GC[AT]^C
AvaII 715 GGTCC G^G[AT]CC
BamHI 1914 GGATCC G^GATCC
BanI 736 GGTGCC G^G[CT][GA]CC
BanII 434 GAGCCC G[GA]GC[CT]^C
BbrPI 1498 CACGTG CAC^GTG
BbsI 1148 GTCTTC GAAGAC(2/6)
BcgI 830 GCAATGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1859 GTATCC GTATCC(6/5)
BfrI 2033 CTTAAG C^TTAAG
BglII 1243 AGATCT A^GATCT
BlpI 80 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 80 GCTTAGC GC^T[ACGT]AGC
BpuAI 1148 GTCTTC GAAGAC(2/6)
BsaWI 71 ACCGGA [AT]^CCGG[AT]
BseRI 431 GAGGAG GAGGAG(10/8)
BsiHKAI 765 GAGCAC G[AT]GC[AT]^C
BsmBI 1228 GAGACG CGTCTC(1/5)
Bsp1407I 2041 TGTACA T^GTACA
BspHI 680 TCATGA T^CATGA
BspLU11I 185 ACATGT A^CATGT
BspMI 2073 ACCTGC ACCTGC(4/8)
BsrDI 830 GCAATG GCAATG(2/0)
BsrGI 2041 TGTACA T^GTACA
Bst1107I 291 GTATAC GTA^TAC
BstAPI 1731 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 114 CGCG CG^CG
BstZ17I 291 GTATAC GTA^TAC
Bsu36I 270 CCTTAGG CC^T[ACGT]AGG
CelII 80 GCTTAGC GC^T[ACGT]AGC
Cfr13I 715 GGTCC G^G[ACGT]CC
DraI 1011 TTTAAA TTT^AAA
DraII 714 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 9 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 270 CCTTAGG CC^T[ACGT]AGG
EcoO109I 714 GGGTCCT [GA]G^G[ACGT]CC[CT]
HapII 72 CCGG C^CGG
HgaI 137 GCGTC GACGC(5/10)
HindIII 1591 AAGCTT A^AGCTT
HpaI 2432 GTTAAC GTT^AAC
HpaII 72 CCGG C^CGG
MspI 72 CCGG C^CGG
MvnI 114 CGCG CG^CG
NcoI 438 CCATGG C^CATGG
NheI 2021 GCTAGC G^CTAGC
PmaCI 1498 CACGTG CAC^GTG
PmlI 1498 CACGTG CAC^GTG
PpuMI 714 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1301 ATTAAT AT^TAAT
Psp1406I 487 AACGTT AA^CGTT
RcaI 680 TCATGA T^CATGA
SalI 56 GTCGAC G^TCGAC
Sau96I 715 GGTCC G^G[ACGT]CC
SnaBI 288 TACGTA TAC^GTA
SspBI 2041 TGTACA T^GTACA
StuI 2158 AGGCCT AGG^CCT
XmnI 397 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 185 ACATGT A^C[GA][CT]GT
1499 ACGTGT
AlwNI 496 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1194 CAGTGTCTG
AspI 1996 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2135 GACAATGTC
BsaAI 288 TACGTA [CT]AC^GT[GA]
1498 CACGTG
BsiYI 1083 CCCGTGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2214 CCACTTCCAGG
BslI 1083 CCCGTGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2214 CCACTTCCAGG
BsmFI 380 GGGAC GGGAC(10/14)
2110 GTCCC
BsmI 1481 GCATTC GAATGC(1/-1)
2562 GCATTC
BstBI 404 TTCGAA TT^CGAA
2445 TTCGAA
BstF5I 1544 CATCC GGATG(2/0)
2183 GGATG
DsaI 438 CCATGG C^C[GA][CT]GG
1084 CCGTGG
EcoRI 626 GAATTC G^AATTC
835 GAATTC
FokI 1544 CATCC GGATG(9/13)
2183 GGATG
NspV 404 TTCGAA TT^CGAA
2445 TTCGAA
PflFI 1996 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2135 GACAATGTC
PleI 977 GACTC GAGTC(4/5)
475 GAGTC
SfuI 404 TTCGAA TT^CGAA
2445 TTCGAA
Tth111I 1996 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2135 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI BbvI BfaI BmyI
BpmI BsaJI BsgI BsmAI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
CfoI DdeI DpnII EcoRII EcoT14I
Fnu4HI HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI PstI PvuII RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII