################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BalI BbeI BbvCI BclI BcnI BglI BlnI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstEII BstPI BstXI Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I KasI KpnI Ksp632I KspI MamI MluI MluNI MroI MscI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflMI PinAI PmeI PshAI PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccII 114 CGCG CG^CG AclI 487 AACGTT AA^CGTT AflII 2033 CTTAAG C^TTAAG AocI 270 CCTTAGG CC^T[ACGT]AGG AseI 1301 ATTAAT AT^TAAT AsnI 1301 ATTAAT AT^TAAT Asp700I 397 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 765 GAGCAC G[AT]GC[AT]^C AvaII 715 GGTCC G^G[AT]CC BamHI 1914 GGATCC G^GATCC BanI 736 GGTGCC G^G[CT][GA]CC BanII 434 GAGCCC G[GA]GC[CT]^C BbrPI 1498 CACGTG CAC^GTG BbsI 1148 GTCTTC GAAGAC(2/6) BcgI 830 GCAATGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1859 GTATCC GTATCC(6/5) BfrI 2033 CTTAAG C^TTAAG BglII 1243 AGATCT A^GATCT BlpI 80 GCTTAGC GC^T[ACGT]AGC Bpu1102I 80 GCTTAGC GC^T[ACGT]AGC BpuAI 1148 GTCTTC GAAGAC(2/6) BsaWI 71 ACCGGA [AT]^CCGG[AT] BseRI 431 GAGGAG GAGGAG(10/8) BsiHKAI 765 GAGCAC G[AT]GC[AT]^C BsmBI 1228 GAGACG CGTCTC(1/5) Bsp1407I 2041 TGTACA T^GTACA BspHI 680 TCATGA T^CATGA BspLU11I 185 ACATGT A^CATGT BspMI 2073 ACCTGC ACCTGC(4/8) BsrDI 830 GCAATG GCAATG(2/0) BsrGI 2041 TGTACA T^GTACA Bst1107I 291 GTATAC GTA^TAC BstAPI 1731 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 114 CGCG CG^CG BstZ17I 291 GTATAC GTA^TAC Bsu36I 270 CCTTAGG CC^T[ACGT]AGG CelII 80 GCTTAGC GC^T[ACGT]AGC Cfr13I 715 GGTCC G^G[ACGT]CC DraI 1011 TTTAAA TTT^AAA DraII 714 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 9 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 270 CCTTAGG CC^T[ACGT]AGG EcoO109I 714 GGGTCCT [GA]G^G[ACGT]CC[CT] HapII 72 CCGG C^CGG HgaI 137 GCGTC GACGC(5/10) HindIII 1591 AAGCTT A^AGCTT HpaI 2432 GTTAAC GTT^AAC HpaII 72 CCGG C^CGG MspI 72 CCGG C^CGG MvnI 114 CGCG CG^CG NcoI 438 CCATGG C^CATGG NheI 2021 GCTAGC G^CTAGC PmaCI 1498 CACGTG CAC^GTG PmlI 1498 CACGTG CAC^GTG PpuMI 714 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 1301 ATTAAT AT^TAAT Psp1406I 487 AACGTT AA^CGTT RcaI 680 TCATGA T^CATGA SalI 56 GTCGAC G^TCGAC Sau96I 715 GGTCC G^G[ACGT]CC SnaBI 288 TACGTA TAC^GTA SspBI 2041 TGTACA T^GTACA StuI 2158 AGGCCT AGG^CCT XmnI 397 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 185 ACATGT A^C[GA][CT]GT 1499 ACGTGT AlwNI 496 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1194 CAGTGTCTG AspI 1996 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2135 GACAATGTC BsaAI 288 TACGTA [CT]AC^GT[GA] 1498 CACGTG BsiYI 1083 CCCGTGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2214 CCACTTCCAGG BslI 1083 CCCGTGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2214 CCACTTCCAGG BsmFI 380 GGGAC GGGAC(10/14) 2110 GTCCC BsmI 1481 GCATTC GAATGC(1/-1) 2562 GCATTC BstBI 404 TTCGAA TT^CGAA 2445 TTCGAA BstF5I 1544 CATCC GGATG(2/0) 2183 GGATG DsaI 438 CCATGG C^C[GA][CT]GG 1084 CCGTGG EcoRI 626 GAATTC G^AATTC 835 GAATTC FokI 1544 CATCC GGATG(9/13) 2183 GGATG NspV 404 TTCGAA TT^CGAA 2445 TTCGAA PflFI 1996 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2135 GACAATGTC PleI 977 GACTC GAGTC(4/5) 475 GAGTC SfuI 404 TTCGAA TT^CGAA 2445 TTCGAA Tth111I 1996 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2135 GACAATGTC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI BbvI BfaI BmyI BpmI BsaJI BsgI BsmAI Bsp1286I BsrI BssKI BstNI BstYI Cac8I CfoI DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NspI PstI PvuII RsaI Sau3AI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII