################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MfeI MflI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 1515 GGTACC G^GTACC AccI 1162 GTCTAC GT^[AC][GT]AC AccII 820 CGCG CG^CG AhdI 2014 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 479 GGGCCC GGGCC^C Asp700I 915 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1515 GGTACC G^GTACC AspEI 2014 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1783 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 1468 GTCTTC GAAGAC(2/6) BciVI 1235 GGATAC GTATCC(6/5) BpuAI 1468 GTCTTC GAAGAC(2/6) BsaBI 681 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2219 GAGACC GGTCTC(1/5) BsmBI 1894 GAGACG CGTCTC(1/5) BsmI 1442 GAATGC GAATGC(1/-1) BsrBI 589 CCGCTC CCGCTC(-3/-3) BssSI 1416 CACGAG CACGAG(-5/-1) BstUI 820 CGCG CG^CG BstXI 71 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 2205 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2014 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 1553 AGCGCC [GA]GCGC^[CT] HgaI 492 GCGTC GACGC(5/10) KpnI 1515 GGTACC GGTAC^C MaeII 1676 ACGT A^CGT MamI 681 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 820 CGCG CG^CG NaeI 1690 GCCGGC GCC^GGC NcoI 119 CCATGG C^CATGG NgoMIV 1690 GCCGGC G^CCGGC PaeR7I 1157 CTCGAG C^TCGAG PflFI 1783 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 50 GGGACCT [GA]G^G[AT]CC[CT] SacI 1092 GAGCTC GAGCT^C SmlI 1157 CTCGAG C^T[CT][GA]AG SphI 1805 GCATGC GCATG^C Tth111I 1783 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1157 CTCGAG C^TCGAG XmnI 915 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BbvCI 600 CCTCAGC CCTCAGC(-5/-2) 756 GCTGAGG BcnI 261 CCGGG CC^[GC]GG 1557 CCGGG BfaI 280 CTAG C^TAG 1213 CTAG BsrDI 1686 CATTGC GCAATG(2/0) 1770 CATTGC DsaI 119 CCATGG C^C[GA][CT]GG 1928 CCACGG MaeI 280 CTAG C^TAG 1213 CTAG MseI 796 TTAA T^TAA 1085 TTAA NciI 261 CCGGG CC^[GC]GG 1557 CCGGG NspI 1056 GCATGT [GA]CATG^[CT] 1805 GCATGC PshAI 1155 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1461 GACAGGGGTC PstI 128 CTGCAG CTGCA^G 2065 CTGCAG Tru9I 796 TTAA T^TAA 1085 TTAA XcmI 221 CCAAGAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 638 CCAACATCCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I