##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KspI MfeI MflI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI XbaI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1515 GGTACC G^GTACC
AccI 1162 GTCTAC GT^[AC][GT]AC
AccII 820 CGCG CG^CG
AhdI 2014 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 479 GGGCCC GGGCC^C
Asp700I 915 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1515 GGTACC G^GTACC
AspEI 2014 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1783 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1468 GTCTTC GAAGAC(2/6)
BciVI 1235 GGATAC GTATCC(6/5)
BpuAI 1468 GTCTTC GAAGAC(2/6)
BsaBI 681 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2219 GAGACC GGTCTC(1/5)
BsmBI 1894 GAGACG CGTCTC(1/5)
BsmI 1442 GAATGC GAATGC(1/-1)
BsrBI 589 CCGCTC CCGCTC(-3/-3)
BssSI 1416 CACGAG CACGAG(-5/-1)
BstUI 820 CGCG CG^CG
BstXI 71 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2205 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2014 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 1553 AGCGCC [GA]GCGC^[CT]
HgaI 492 GCGTC GACGC(5/10)
KpnI 1515 GGTACC GGTAC^C
MaeII 1676 ACGT A^CGT
MamI 681 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 820 CGCG CG^CG
NaeI 1690 GCCGGC GCC^GGC
NcoI 119 CCATGG C^CATGG
NgoMIV 1690 GCCGGC G^CCGGC
PaeR7I 1157 CTCGAG C^TCGAG
PflFI 1783 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 50 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1092 GAGCTC GAGCT^C
SmlI 1157 CTCGAG C^T[CT][GA]AG
SphI 1805 GCATGC GCATG^C
Tth111I 1783 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1157 CTCGAG C^TCGAG
XmnI 915 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BbvCI 600 CCTCAGC CCTCAGC(-5/-2)
756 GCTGAGG
BcnI 261 CCGGG CC^[GC]GG
1557 CCGGG
BfaI 280 CTAG C^TAG
1213 CTAG
BsrDI 1686 CATTGC GCAATG(2/0)
1770 CATTGC
DsaI 119 CCATGG C^C[GA][CT]GG
1928 CCACGG
MaeI 280 CTAG C^TAG
1213 CTAG
MseI 796 TTAA T^TAA
1085 TTAA
NciI 261 CCGGG CC^[GC]GG
1557 CCGGG
NspI 1056 GCATGT [GA]CATG^[CT]
1805 GCATGC
PshAI 1155 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1461 GACAGGGGTC
PstI 128 CTGCAG CTGCA^G
2065 CTGCAG
Tru9I 796 TTAA T^TAA
1085 TTAA
XcmI 221 CCAAGAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
638 CCAACATCCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbvI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I