##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
BclI BfrI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI Eco81I EcoNI EcoRI EcoRV
FbaI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1413 GTAGAC GT^[AC][GT]AC
AcyI 2898 GGCGCC G[GA]^CG[CT]C
AfaI 2039 GTAC GT^AC
AflIII 1996 ACATGT A^C[GA][CT]GT
AhdI 1889 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1520 AGCGCT AGC^GCT
AspEI 1889 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1114 TGCGCA TGC^GCA
AvrII 1430 CCTAGG C^CTAGG
BamHI 645 GGATCC G^GATCC
BbeI 2898 GGCGCC GGCGC^C
BbrPI 919 CACGTG CAC^GTG
BcgI 2860 GCACCTCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2333 GTATCC GTATCC(6/5)
BglII 2068 AGATCT A^GATCT
BlnI 1430 CCTAGG C^CTAGG
BsaAI 919 CACGTG [CT]AC^GT[GA]
BsaHI 2898 GGCGCC G[GA]^CG[CT]C
BspHI 2767 TCATGA T^CATGA
BspLU11I 1996 ACATGT A^CATGT
BstEII 264 GGTCACC G^GT[ACGT]ACC
BstPI 264 GGTCACC G^GT[ACGT]ACC
DraI 3034 TTTAAA TTT^AAA
Eam1105I 1889 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1520 AGCGCT AGC^GCT
EcoO65I 264 GGTCACC G^GT[ACGT]ACC
EcoT22I 1211 ATGCAT ATGCA^T
FspI 1114 TGCGCA TGC^GCA
Hin1I 2898 GGCGCC G[GA]^CG[CT]C
KasI 2898 GGCGCC G^GCGCC
KspI 112 CCGCGG CCGC^GG
MslI 1343 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2898 GGCGCC GG^CGCC
NcoI 2438 CCATGG C^CATGG
NsiI 1211 ATGCAT ATGCA^T
PaeR7I 2355 CTCGAG C^TCGAG
PflMI 1028 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 919 CACGTG CAC^GTG
PmlI 919 CACGTG CAC^GTG
PstI 2187 CTGCAG CTGCA^G
RcaI 2767 TCATGA T^CATGA
RsaI 2039 GTAC GT^AC
SacII 112 CCGCGG CCGC^GG
SapI 793 GCTCTTC GCTCTTC(1/4)
SexAI 1672 ACCTGGT A^CC[AT]GGT
SfoI 2898 GGCGCC GGC^GCC
SphI 1209 GCATGC GCATG^C
Van91I 1028 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2355 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 280 GGGCCC GGGCC^C
2443 GGGCCC
AspHI 479 GAGCTC G[AT]GC[AT]^C
1005 GAGCTC
BalI 923 TGGCCA TGG^CCA
1693 TGGCCA
BanI 81 GGCACC G^G[CT][GA]CC
2898 GGCGCC
BbvCI 325 GCTGAGG CCTCAGC(-5/-2)
2594 GCTGAGG
BfaI 1431 CTAG C^TAG
2217 CTAG
BsaI 66 GAGACC GGTCTC(1/5)
2201 GGTCTC
BsiHKAI 479 GAGCTC G[AT]GC[AT]^C
1005 GAGCTC
BstXI 405 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
860 CCAGTTCTCTGG
DrdI 1080 GACACCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2501 GACCCCTCTGTC
EcoT14I 1430 CCTAGG C^C[AT][AT]GG
2438 CCATGG
FseI 133 GGCCGGCC GGCCGG^CC
598 GGCCGGCC
HincII 1089 GTCAAC GT[CT]^[GA]AC
2013 GTCAAC
HindII 1089 GTCAAC GT[CT]^[GA]AC
2013 GTCAAC
MaeI 1431 CTAG C^TAG
2217 CTAG
MaeII 920 ACGT A^CGT
2017 ACGT
MluNI 923 TGGCCA TGG^CCA
1693 TGGCCA
MscI 923 TGGCCA TGG^CCA
1693 TGGCCA
NruI 149 TCGCGA TCG^CGA
2273 TCGCGA
NspI 1209 GCATGC [GA]CATG^[CT]
1996 ACATGT
PpuMI 415 GGGTCCC [GA]G^G[AT]CC[CT]
2417 GGGTCCC
PshAI 68 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
442 GACCGCCGTC
PvuII 1353 CAGCTG CAG^CTG
1533 CAGCTG
SacI 479 GAGCTC GAGCT^C
1005 GAGCTC
SmlI 1293 CTCAAG C^T[CT][GA]AG
2355 CTCGAG
StyI 1430 CCTAGG C^C[AT][AT]GG
2438 CCATGG
TfiI 1598 GATTC G^A[AT]TC
2406 GATTC
XcmI 1459 CCAAGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1976 CCAGGCGGAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AvaI AvaII BanII
BbsI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII