##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII BanII
BbrPI BclI BglI BglII BsaBI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
FspI KpnI KspI MamI MfeI
MroI MunI NcoI NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 744 GACGTC GACGT^C
AccI 3554 GTAGAC GT^[AC][GT]AC
AflII 1493 CTTAAG C^TTAAG
Alw44I 1051 GTGCAC G^TGCAC
AlwNI 919 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1051 GTGCAC G^TGCAC
Asp700I 825 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1755 CCTAGG C^CTAGG
BalI 734 TGGCCA TGG^CCA
BamHI 857 GGATCC G^GATCC
BanI 1729 GGCGCC G^G[CT][GA]CC
BbeI 1729 GGCGCC GGCGC^C
BbvCI 1746 GCTGAGG CCTCAGC(-5/-2)
BciVI 442 GTATCC GTATCC(6/5)
BfrI 1493 CTTAAG C^TTAAG
BlnI 1755 CCTAGG C^CTAGG
BlpI 1686 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1686 GCTGAGC GC^T[ACGT]AGC
BsaI 997 GAGACC GGTCTC(1/5)
BsaWI 604 TCCGGT [AT]^CCGG[AT]
BseRI 1064 GAGGAG GAGGAG(10/8)
BsmBI 1135 GAGACG CGTCTC(1/5)
BspHI 2302 TCATGA T^CATGA
BspMI 3586 ACCTGC ACCTGC(4/8)
BsrBI 54 GAGCGG CCGCTC(-3/-3)
BsrFI 1739 GCCGGC [GA]^CCGG[CT]
CelII 1686 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1739 GCCGGC [GA]^CCGG[CT]
DraIII 3283 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1287 GACAGAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 734 TGGCCA [CT]^GGCC[GA]
EcoT22I 618 ATGCAT ATGCA^T
HindIII 1620 AAGCTT A^AGCTT
HpaI 2667 GTTAAC GTT^AAC
KasI 1729 GGCGCC G^GCGCC
MluI 1138 ACGCGT A^CGCGT
MluNI 734 TGGCCA TGG^CCA
MscI 734 TGGCCA TGG^CCA
MspA1I 1542 CAGCTG C[AC]G^C[GT]G
NaeI 1739 GCCGGC GCC^GGC
NarI 1729 GGCGCC GG^CGCC
NgoMIV 1739 GCCGGC G^CCGGC
NsiI 618 ATGCAT ATGCA^T
PaeR7I 994 CTCGAG C^TCGAG
PleI 2578 GACTC GAGTC(4/5)
PpuMI 1347 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1542 CAGCTG CAG^CTG
RcaI 2302 TCATGA T^CATGA
SfoI 1729 GGCGCC GGC^GCC
SnaBI 3029 TACGTA TAC^GTA
StuI 531 AGGCCT AGG^CCT
XbaI 833 TCTAGA T^CTAGA
XcmI 1106 CCAACAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 994 CTCGAG C^TCGAG
XmnI 825 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaI 190 CCCGAG C^[CT]CG[GA]G
994 CTCGAG
AvaII 1348 GGACC G^G[AT]CC
1451 GGACC
BcgI 1932 CGAGTGTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1079 GCAGGACGTTCG
BcnI 288 CCGGG CC^[GC]GG
446 CCGGG
BsaAI 1956 CACGTA [CT]AC^GT[GA]
3029 TACGTA
BsmI 1200 GAATGC GAATGC(1/-1)
2770 GCATTC
BsoBI 190 CCCGAG C^[CT]CG[GA]G
994 CTCGAG
BspLU11I 433 ACATGT A^CATGT
958 ACATGT
BsrDI 2490 CATTGC GCAATG(2/0)
2526 GCAATG
BstAPI 1021 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1709 GCACACGCTGC
BstYI 353 AGATCC [GA]^GATC[CT]
857 GGATCC
DraII 482 AGGCCCT [GA]G^G[ACGT]CC[CT]
1347 GGGACCT
DsaI 709 CCGTGG C^C[GA][CT]GG
1521 CCGTGG
EcoNI 523 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1027 CCTGCTCCAGG
EcoO109I 482 AGGCCCT [GA]G^G[ACGT]CC[CT]
1347 GGGACCT
FauI 1208 CCCGC CCCGC(4/6)
2750 CCCGC
HaeII 152 GGCGCT [GA]GCGC^[CT]
1729 GGCGCC
HincII 2667 GTTAAC GT[CT]^[GA]AC
3240 GTTGAC
HindII 2667 GTTAAC GT[CT]^[GA]AC
3240 GTTGAC
MflI 353 AGATCC [GA]^GATC[CT]
857 GGATCC
NciI 288 CCGGG CC^[GC]GG
446 CCGGG
NdeI 466 CATATG CA^TATG
1970 CATATG
SapI 1419 GAAGAGC GCTCTTC(1/4)
1316 GCTCTTC
SphI 1805 GCATGC GCATG^C
2624 GCATGC
Tsp45I 500 GTCAC ^GT[GC]AC
3141 GTGAC
XhoII 353 AGATCC [GA]^GATC[CT]
857 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AocI ApoI
AspHI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
Eco81I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PflMI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SpeI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I