################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII BanII BbrPI BclI BglI BglII BsaBI BseAI BsgI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI KpnI KspI MamI MfeI MroI MunI NcoI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 744 GACGTC GACGT^C AccI 3554 GTAGAC GT^[AC][GT]AC AflII 1493 CTTAAG C^TTAAG Alw44I 1051 GTGCAC G^TGCAC AlwNI 919 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1051 GTGCAC G^TGCAC Asp700I 825 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1755 CCTAGG C^CTAGG BalI 734 TGGCCA TGG^CCA BamHI 857 GGATCC G^GATCC BanI 1729 GGCGCC G^G[CT][GA]CC BbeI 1729 GGCGCC GGCGC^C BbvCI 1746 GCTGAGG CCTCAGC(-5/-2) BciVI 442 GTATCC GTATCC(6/5) BfrI 1493 CTTAAG C^TTAAG BlnI 1755 CCTAGG C^CTAGG BlpI 1686 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1686 GCTGAGC GC^T[ACGT]AGC BsaI 997 GAGACC GGTCTC(1/5) BsaWI 604 TCCGGT [AT]^CCGG[AT] BseRI 1064 GAGGAG GAGGAG(10/8) BsmBI 1135 GAGACG CGTCTC(1/5) BspHI 2302 TCATGA T^CATGA BspMI 3586 ACCTGC ACCTGC(4/8) BsrBI 54 GAGCGG CCGCTC(-3/-3) BsrFI 1739 GCCGGC [GA]^CCGG[CT] CelII 1686 GCTGAGC GC^T[ACGT]AGC Cfr10I 1739 GCCGGC [GA]^CCGG[CT] DraIII 3283 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1287 GACAGAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 734 TGGCCA [CT]^GGCC[GA] EcoT22I 618 ATGCAT ATGCA^T HindIII 1620 AAGCTT A^AGCTT HpaI 2667 GTTAAC GTT^AAC KasI 1729 GGCGCC G^GCGCC MluI 1138 ACGCGT A^CGCGT MluNI 734 TGGCCA TGG^CCA MscI 734 TGGCCA TGG^CCA MspA1I 1542 CAGCTG C[AC]G^C[GT]G NaeI 1739 GCCGGC GCC^GGC NarI 1729 GGCGCC GG^CGCC NgoMIV 1739 GCCGGC G^CCGGC NsiI 618 ATGCAT ATGCA^T PaeR7I 994 CTCGAG C^TCGAG PleI 2578 GACTC GAGTC(4/5) PpuMI 1347 GGGACCT [GA]G^G[AT]CC[CT] PvuII 1542 CAGCTG CAG^CTG RcaI 2302 TCATGA T^CATGA SfoI 1729 GGCGCC GGC^GCC SnaBI 3029 TACGTA TAC^GTA StuI 531 AGGCCT AGG^CCT XbaI 833 TCTAGA T^CTAGA XcmI 1106 CCAACAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 994 CTCGAG C^TCGAG XmnI 825 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AvaI 190 CCCGAG C^[CT]CG[GA]G 994 CTCGAG AvaII 1348 GGACC G^G[AT]CC 1451 GGACC BcgI 1932 CGAGTGTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1079 GCAGGACGTTCG BcnI 288 CCGGG CC^[GC]GG 446 CCGGG BsaAI 1956 CACGTA [CT]AC^GT[GA] 3029 TACGTA BsmI 1200 GAATGC GAATGC(1/-1) 2770 GCATTC BsoBI 190 CCCGAG C^[CT]CG[GA]G 994 CTCGAG BspLU11I 433 ACATGT A^CATGT 958 ACATGT BsrDI 2490 CATTGC GCAATG(2/0) 2526 GCAATG BstAPI 1021 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1709 GCACACGCTGC BstYI 353 AGATCC [GA]^GATC[CT] 857 GGATCC DraII 482 AGGCCCT [GA]G^G[ACGT]CC[CT] 1347 GGGACCT DsaI 709 CCGTGG C^C[GA][CT]GG 1521 CCGTGG EcoNI 523 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1027 CCTGCTCCAGG EcoO109I 482 AGGCCCT [GA]G^G[ACGT]CC[CT] 1347 GGGACCT FauI 1208 CCCGC CCCGC(4/6) 2750 CCCGC HaeII 152 GGCGCT [GA]GCGC^[CT] 1729 GGCGCC HincII 2667 GTTAAC GT[CT]^[GA]AC 3240 GTTGAC HindII 2667 GTTAAC GT[CT]^[GA]AC 3240 GTTGAC MflI 353 AGATCC [GA]^GATC[CT] 857 GGATCC NciI 288 CCGGG CC^[GC]GG 446 CCGGG NdeI 466 CATATG CA^TATG 1970 CATATG SapI 1419 GAAGAGC GCTCTTC(1/4) 1316 GCTCTTC SphI 1805 GCATGC GCATG^C 2624 GCATGC Tsp45I 500 GTCAC ^GT[GC]AC 3141 GTGAC XhoII 353 AGATCC [GA]^GATC[CT] 857 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AocI ApoI AspHI BbsI BbvI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI EarI Eco81I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PflMI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I