##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Aor51HI AscI AseI AsnI
AvaI AviII BbeI BbrPI BcgI
BciVI BclI BfrI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BsrBI BsrFI
Bst1107I BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII Hin1I HpaI KasI KspI
MluI MroI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SmaI SpeI SphI
Sse8387I SwaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 331 GGTACC G^GTACC
AccI 1409 GTAGAC GT^[AC][GT]AC
AflIII 1149 ACATGT A^C[GA][CT]GT
AhdI 1390 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1765 CCTTAGG CC^T[ACGT]AGG
Asp718I 331 GGTACC G^GTACC
AspEI 1390 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2946 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2635 CCTAGG C^CTAGG
BalI 364 TGGCCA TGG^CCA
BanI 331 GGTACC G^G[CT][GA]CC
BbvCI 50 GCTGAGG CCTCAGC(-5/-2)
BglI 1076 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 926 AGATCT A^GATCT
BlnI 2635 CCTAGG C^CTAGG
BsaAI 1884 TACGTA [CT]AC^GT[GA]
BsaBI 1783 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 491 GGTCTC GGTCTC(1/5)
BsmBI 3112 GAGACG CGTCTC(1/5)
Bsp1407I 994 TGTACA T^GTACA
BspHI 604 TCATGA T^CATGA
BspLU11I 1149 ACATGT A^CATGT
BsrGI 994 TGTACA T^GTACA
BssSI 3096 CTCGTG CACGAG(-5/-1)
BstAPI 1207 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 349 TTCGAA TT^CGAA
Bsu36I 1765 CCTTAGG CC^T[ACGT]AGG
DraIII 1590 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2663 CCACGG C^C[GA][CT]GG
Eam1105I 1390 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1765 CCTTAGG CC^T[ACGT]AGG
EcoNI 3159 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3088 ATGCAT ATGCA^T
HincII 1819 GTTGAC GT[CT]^[GA]AC
HindII 1819 GTTGAC GT[CT]^[GA]AC
HindIII 2284 AAGCTT A^AGCTT
KpnI 331 GGTACC GGTAC^C
MamI 1783 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1658 CAATTG C^AATTG
MluNI 364 TGGCCA TGG^CCA
MscI 364 TGGCCA TGG^CCA
MunI 1658 CAATTG C^AATTG
NdeI 1991 CATATG CA^TATG
NsiI 3088 ATGCAT ATGCA^T
NspV 349 TTCGAA TT^CGAA
PflFI 2946 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2898 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 604 TCATGA T^CATGA
SapI 2912 GAAGAGC GCTCTTC(1/4)
ScaI 2454 AGTACT AGT^ACT
SexAI 2947 ACCAGGT A^CC[AT]GGT
SfuI 349 TTCGAA TT^CGAA
SnaBI 1884 TACGTA TAC^GTA
SspBI 994 TGTACA T^GTACA
SspI 449 AATATT AAT^ATT
StuI 3167 AGGCCT AGG^CCT
Tth111I 2946 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 557 GTGCAC G^TGCAC
2448 GTGCAC
ApaLI 557 GTGCAC G^TGCAC
2448 GTGCAC
BamHI 265 GGATCC G^GATCC
2781 GGATCC
BcnI 1104 CCGGG CC^[GC]GG
2366 CCGGG
BsrDI 3091 CATTGC GCAATG(2/0)
3248 GCAATG
BssHII 1245 GCGCGC G^CGCGC
1247 GCGCGC
EaeI 364 TGGCCA [CT]^GGCC[GA]
1101 TGGCCG
EcoRI 1980 GAATTC G^AATTC
2407 GAATTC
EcoT14I 1687 CCAAGG C^C[AT][AT]GG
2635 CCTAGG
HgaI 44 GCGTC GACGC(5/10)
3070 GCGTC
MaeII 1885 ACGT A^CGT
2249 ACGT
MslI 889 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1028 CATTTCTGTG
NciI 1104 CCGGG CC^[GC]GG
2366 CCGGG
SfaNI 2148 GATGC GCATC(5/9)
601 GCATC
SmlI 2375 CTCAAG C^T[CT][GA]AG
3059 CTTGAG
StyI 1687 CCAAGG C^C[AT][AT]GG
2635 CCTAGG
TaqI 350 TCGA T^CGA
392 TCGA
TthHB8I 350 TCGA T^CGA
392 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XhoII XmnI