##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BcgI BciVI BclI BfrI
BglII BlnI BsaHI BsiWI BsmI
Bsp1407I BspDI BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 763 CGCG CG^CG
AccIII 345 TCCGGA T^CCGGA
AflIII 141 ACATGT A^C[GA][CT]GT
AhdI 15 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1637 GTGCAC G^TGCAC
ApaLI 1637 GTGCAC G^TGCAC
Asp700I 2066 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 15 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 877 CACGTG CAC^GTG
BbvCI 979 GCTGAGG CCTCAGC(-5/-2)
BglI 1307 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 877 CACGTG [CT]AC^GT[GA]
BsaBI 2028 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1124 GAGACC GGTCTC(1/5)
BseAI 345 TCCGGA T^CCGGA
BsgI 958 CTGCAC GTGCAG(16/14)
BsmBI 2526 GAGACG CGTCTC(1/5)
BspEI 345 TCCGGA T^CCGGA
BspHI 1076 TCATGA T^CATGA
BspLU11I 141 ACATGT A^CATGT
BsrBI 181 GAGCGG CCGCTC(-3/-3)
BssSI 310 CTCGTG CACGAG(-5/-1)
BstUI 763 CGCG CG^CG
DraIII 546 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 15 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 1904 AAGCTT A^AGCTT
KspI 762 CCGCGG CCGC^GG
MamI 2028 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 345 TCCGGA T^CCGGA
MvnI 763 CGCG CG^CG
PmaCI 877 CACGTG CAC^GTG
PmlI 877 CACGTG CAC^GTG
PpuMI 737 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 1076 TCATGA T^CATGA
SacII 762 CCGCGG CCGC^GG
ScaI 533 AGTACT AGT^ACT
SmaI 1277 CCCGGG CCC^GGG
SmlI 2201 CTCAAG C^T[CT][GA]AG
SpeI 2279 ACTAGT A^CTAGT
SphI 2576 GCATGC GCATG^C
StuI 684 AGGCCT AGG^CCT
XcmI 92 CCAAGCCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1277 CCCGGG C^CCGGG
XmaI 1277 CCCGGG C^CCGGG
XmnI 2066 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 2779 AAATTT [GA]^AATT[CT]
2790 AAATTT
AlwNI 1819 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2207 CAGTTGCTG
ApaI 380 GGGCCC GGGCC^C
867 GGGCCC
ApoI 2779 AAATTT [GA]^AATT[CT]
2790 AAATTT
AspHI 269 GAGCAC G[AT]GC[AT]^C
1637 GTGCAC
AspI 1126 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1803 GACTGGGTC
BamHI 1085 GGATCC G^GATCC
2444 GGATCC
BlpI 1342 GCTCAGC GC^T[ACGT]AGC
1485 GCTCAGC
Bpu1102I 1342 GCTCAGC GC^T[ACGT]AGC
1485 GCTCAGC
BseRI 982 GAGGAG GAGGAG(10/8)
1632 GAGGAG
BsiHKAI 269 GAGCAC G[AT]GC[AT]^C
1637 GTGCAC
BsrDI 1266 CATTGC GCAATG(2/0)
1508 CATTGC
BstAPI 2206 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2232 GCATTTAATGC
BstEII 22 GGTCACC G^GT[ACGT]ACC
907 GGTGACC
BstPI 22 GGTCACC G^GT[ACGT]ACC
907 GGTGACC
BstYI 1085 GGATCC [GA]^GATC[CT]
2444 GGATCC
CelII 1342 GCTCAGC GC^T[ACGT]AGC
1485 GCTCAGC
DrdI 480 GACAGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2431 GACCAAGGTGTC
EcoO65I 22 GGTCACC G^GT[ACGT]ACC
907 GGTGACC
MaeII 878 ACGT A^CGT
2154 ACGT
MflI 1085 GGATCC [GA]^GATC[CT]
2444 GGATCC
PflFI 1126 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1803 GACTGGGTC
PflMI 412 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCAGTGGGTGG
SapI 1005 GAAGAGC GCTCTTC(1/4)
1206 GCTCTTC
Tth111I 1126 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1803 GACTGGGTC
Van91I 412 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCAGTGGGTGG
XhoII 1085 GGATCC [GA]^GATC[CT]
2444 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI