##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI AspI
AviII AvrII BamHI BciVI BfrI
BglI BlnI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Cfr10I CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FseI FspI HincII HindII
HpaI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 964 GGTACC G^GTACC
AccIII 3323 TCCGGA T^CCGGA
AcsI 3177 AAATTC [GA]^AATT[CT]
Alw44I 2862 GTGCAC G^TGCAC
ApaI 905 GGGCCC GGGCC^C
ApaLI 2862 GTGCAC G^TGCAC
ApoI 3177 AAATTC [GA]^AATT[CT]
Asp718I 964 GGTACC G^GTACC
BalI 461 TGGCCA TGG^CCA
BbeI 13 GGCGCC GGCGC^C
BbrPI 2421 CACGTG CAC^GTG
BclI 262 TGATCA T^GATCA
BglII 2239 AGATCT A^GATCT
BsaBI 1846 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3323 TCCGGA [AT]^CCGG[AT]
BseAI 3323 TCCGGA T^CCGGA
BspDI 621 ATCGAT AT^CGAT
BspEI 3323 TCCGGA T^CCGGA
BsrBI 3078 GAGCGG CCGCTC(-3/-3)
BsrDI 2553 GCAATG GCAATG(2/0)
BstAPI 102 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 621 ATCGAT AT^CGAT
DraI 3383 TTTAAA TTT^AAA
DrdI 1035 GACAAGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 401 GATATC GAT^ATC
FbaI 262 TGATCA T^GATCA
KasI 13 GGCGCC G^GCGCC
KpnI 964 GGTACC GGTAC^C
KspI 70 CCGCGG CCGC^GG
MamI 1846 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 461 TGGCCA TGG^CCA
MroI 3323 TCCGGA T^CCGGA
MscI 461 TGGCCA TGG^CCA
NarI 13 GGCGCC GG^CGCC
NcoI 9 CCATGG C^CATGG
PmaCI 2421 CACGTG CAC^GTG
PmlI 2421 CACGTG CAC^GTG
PstI 280 CTGCAG CTGCA^G
SacII 70 CCGCGG CCGC^GG
ScaI 1900 AGTACT AGT^ACT
SfoI 13 GGCGCC GGC^GCC
SmaI 40 CCCGGG CCC^GGG
SphI 3341 GCATGC GCATG^C
XbaI 1322 TCTAGA T^CTAGA
XcmI 3053 CCAGACGTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 40 CCCGGG C^CCGGG
XmaI 40 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 13 GGCGCC G[GA]^CG[CT]C
80 GACGCC
AflIII 1435 ACACGT A^C[GA][CT]GT
1591 ACACGT
AhdI 2427 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACACTTGGTC
AlwNI 308 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1803 CAGCGCCTG
AocI 2187 CCTGAGG CC^T[ACGT]AGG
2741 CCTCAGG
AspEI 2427 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACACTTGGTC
BanI 13 GGCGCC G^G[CT][GA]CC
964 GGTACC
BlpI 248 GCTCAGC GC^T[ACGT]AGC
638 GCTCAGC
Bpu1102I 248 GCTCAGC GC^T[ACGT]AGC
638 GCTCAGC
BsaAI 1947 TACGTG [CT]AC^GT[GA]
2421 CACGTG
BsaHI 13 GGCGCC G[GA]^CG[CT]C
80 GACGCC
BsgI 1920 CTGCAC GTGCAG(16/14)
2286 GTGCAG
BsiEI 46 CGACCG CG[GA][CT]^CG
2397 CGACCG
BspHI 2002 TCATGA T^CATGA
2296 TCATGA
Bsu36I 2187 CCTGAGG CC^T[ACGT]AGG
2741 CCTCAGG
CelII 248 GCTCAGC GC^T[ACGT]AGC
638 GCTCAGC
Eam1105I 2427 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACACTTGGTC
Eco81I 2187 CCTGAGG CC^T[ACGT]AGG
2741 CCTCAGG
EcoNI 187 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1829 CCTGAAGCAGG
HgaI 80 GACGC GACGC(5/10)
2305 GCGTC
Hin1I 13 GGCGCC G[GA]^CG[CT]C
80 GACGCC
HindIII 1521 AAGCTT A^AGCTT
2781 AAGCTT
PflMI 1802 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2549 CCACGCAATGG
PpuMI 1542 GGGTCCC [GA]G^G[AT]CC[CT]
3377 GGGACCT
RcaI 2002 TCATGA T^CATGA
2296 TCATGA
SmlI 2885 CTTGAG C^T[CT][GA]AG
3183 CTCAAG
Van91I 1802 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2549 CCACGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI