################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AccI AclI AflII AgeI Aor51HI AscI AseI AsnI AspI AviII AvrII BamHI BciVI BfrI BglI BlnI BsiWI BsmBI BsmI Bsp1407I BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Cfr10I CpoI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HincII HindII HpaI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 964 GGTACC G^GTACC AccIII 3323 TCCGGA T^CCGGA AcsI 3177 AAATTC [GA]^AATT[CT] Alw44I 2862 GTGCAC G^TGCAC ApaI 905 GGGCCC GGGCC^C ApaLI 2862 GTGCAC G^TGCAC ApoI 3177 AAATTC [GA]^AATT[CT] Asp718I 964 GGTACC G^GTACC BalI 461 TGGCCA TGG^CCA BbeI 13 GGCGCC GGCGC^C BbrPI 2421 CACGTG CAC^GTG BclI 262 TGATCA T^GATCA BglII 2239 AGATCT A^GATCT BsaBI 1846 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3323 TCCGGA [AT]^CCGG[AT] BseAI 3323 TCCGGA T^CCGGA BspDI 621 ATCGAT AT^CGAT BspEI 3323 TCCGGA T^CCGGA BsrBI 3078 GAGCGG CCGCTC(-3/-3) BsrDI 2553 GCAATG GCAATG(2/0) BstAPI 102 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 621 ATCGAT AT^CGAT DraI 3383 TTTAAA TTT^AAA DrdI 1035 GACAAGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 401 GATATC GAT^ATC FbaI 262 TGATCA T^GATCA KasI 13 GGCGCC G^GCGCC KpnI 964 GGTACC GGTAC^C KspI 70 CCGCGG CCGC^GG MamI 1846 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 461 TGGCCA TGG^CCA MroI 3323 TCCGGA T^CCGGA MscI 461 TGGCCA TGG^CCA NarI 13 GGCGCC GG^CGCC NcoI 9 CCATGG C^CATGG PmaCI 2421 CACGTG CAC^GTG PmlI 2421 CACGTG CAC^GTG PstI 280 CTGCAG CTGCA^G SacII 70 CCGCGG CCGC^GG ScaI 1900 AGTACT AGT^ACT SfoI 13 GGCGCC GGC^GCC SmaI 40 CCCGGG CCC^GGG SphI 3341 GCATGC GCATG^C XbaI 1322 TCTAGA T^CTAGA XcmI 3053 CCAGACGTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 40 CCCGGG C^CCGGG XmaI 40 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 13 GGCGCC G[GA]^CG[CT]C 80 GACGCC AflIII 1435 ACACGT A^C[GA][CT]GT 1591 ACACGT AhdI 2427 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2461 GACACTTGGTC AlwNI 308 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 1803 CAGCGCCTG AocI 2187 CCTGAGG CC^T[ACGT]AGG 2741 CCTCAGG AspEI 2427 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2461 GACACTTGGTC BanI 13 GGCGCC G^G[CT][GA]CC 964 GGTACC BlpI 248 GCTCAGC GC^T[ACGT]AGC 638 GCTCAGC Bpu1102I 248 GCTCAGC GC^T[ACGT]AGC 638 GCTCAGC BsaAI 1947 TACGTG [CT]AC^GT[GA] 2421 CACGTG BsaHI 13 GGCGCC G[GA]^CG[CT]C 80 GACGCC BsgI 1920 CTGCAC GTGCAG(16/14) 2286 GTGCAG BsiEI 46 CGACCG CG[GA][CT]^CG 2397 CGACCG BspHI 2002 TCATGA T^CATGA 2296 TCATGA Bsu36I 2187 CCTGAGG CC^T[ACGT]AGG 2741 CCTCAGG CelII 248 GCTCAGC GC^T[ACGT]AGC 638 GCTCAGC Eam1105I 2427 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2461 GACACTTGGTC Eco81I 2187 CCTGAGG CC^T[ACGT]AGG 2741 CCTCAGG EcoNI 187 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1829 CCTGAAGCAGG HgaI 80 GACGC GACGC(5/10) 2305 GCGTC Hin1I 13 GGCGCC G[GA]^CG[CT]C 80 GACGCC HindIII 1521 AAGCTT A^AGCTT 2781 AAGCTT PflMI 1802 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2549 CCACGCAATGG PpuMI 1542 GGGTCCC [GA]G^G[AT]CC[CT] 3377 GGGACCT RcaI 2002 TCATGA T^CATGA 2296 TCATGA SmlI 2885 CTTGAG C^T[CT][GA]AG 3183 CTCAAG Van91I 1802 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2549 CCACGCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AfaI AluI AlwI Asp700I AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI