##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       AccI        AclI        AflII       AgeI        
Aor51HI     AscI        AseI        AsnI        AspI        
AviII       AvrII       BamHI       BciVI       BfrI        
BglI        BlnI        BsiWI       BsmBI       BsmI        
Bsp1407I    BspLU11I    BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Cfr10I      CpoI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoT22I     FseI        FspI        HincII      HindII      
HpaI        MfeI        MluI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SapI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        964  GGTACC               G^GTACC
AccIII       3323  TCCGGA               T^CCGGA
AcsI         3177  AAATTC               [GA]^AATT[CT]
Alw44I       2862  GTGCAC               G^TGCAC
ApaI          905  GGGCCC               GGGCC^C
ApaLI        2862  GTGCAC               G^TGCAC
ApoI         3177  AAATTC               [GA]^AATT[CT]
Asp718I       964  GGTACC               G^GTACC
BalI          461  TGGCCA               TGG^CCA
BbeI           13  GGCGCC               GGCGC^C
BbrPI        2421  CACGTG               CAC^GTG
BclI          262  TGATCA               T^GATCA
BglII        2239  AGATCT               A^GATCT
BsaBI        1846  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3323  TCCGGA               [AT]^CCGG[AT]
BseAI        3323  TCCGGA               T^CCGGA
BspDI         621  ATCGAT               AT^CGAT
BspEI        3323  TCCGGA               T^CCGGA
BsrBI        3078  GAGCGG               CCGCTC(-3/-3)
BsrDI        2553  GCAATG               GCAATG(2/0)
BstAPI        102  GCAGAAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI          621  ATCGAT               AT^CGAT
DraI         3383  TTTAAA               TTT^AAA
DrdI         1035  GACAAGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         401  GATATC               GAT^ATC
FbaI          262  TGATCA               T^GATCA
KasI           13  GGCGCC               G^GCGCC
KpnI          964  GGTACC               GGTAC^C
KspI           70  CCGCGG               CCGC^GG
MamI         1846  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         461  TGGCCA               TGG^CCA
MroI         3323  TCCGGA               T^CCGGA
MscI          461  TGGCCA               TGG^CCA
NarI           13  GGCGCC               GG^CGCC
NcoI            9  CCATGG               C^CATGG
PmaCI        2421  CACGTG               CAC^GTG
PmlI         2421  CACGTG               CAC^GTG
PstI          280  CTGCAG               CTGCA^G
SacII          70  CCGCGG               CCGC^GG
ScaI         1900  AGTACT               AGT^ACT
SfoI           13  GGCGCC               GGC^GCC
SmaI           40  CCCGGG               CCC^GGG
SphI         3341  GCATGC               GCATG^C
XbaI         1322  TCTAGA               T^CTAGA
XcmI         3053  CCAGACGTTCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          40  CCCGGG               C^CCGGG
XmaI           40  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI           13  GGCGCC               G[GA]^CG[CT]C
               80  GACGCC              
AflIII       1435  ACACGT               A^C[GA][CT]GT
             1591  ACACGT              
AhdI         2427  GACTTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2461  GACACTTGGTC         
AlwNI         308  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1803  CAGCGCCTG           
AocI         2187  CCTGAGG              CC^T[ACGT]AGG
             2741  CCTCAGG             
AspEI        2427  GACTTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2461  GACACTTGGTC         
BanI           13  GGCGCC               G^G[CT][GA]CC
              964  GGTACC              
BlpI          248  GCTCAGC              GC^T[ACGT]AGC
              638  GCTCAGC             
Bpu1102I      248  GCTCAGC              GC^T[ACGT]AGC
              638  GCTCAGC             
BsaAI        1947  TACGTG               [CT]AC^GT[GA]
             2421  CACGTG              
BsaHI          13  GGCGCC               G[GA]^CG[CT]C
               80  GACGCC              
BsgI         1920  CTGCAC               GTGCAG(16/14)
             2286  GTGCAG              
BsiEI          46  CGACCG               CG[GA][CT]^CG
             2397  CGACCG              
BspHI        2002  TCATGA               T^CATGA
             2296  TCATGA              
Bsu36I       2187  CCTGAGG              CC^T[ACGT]AGG
             2741  CCTCAGG             
CelII         248  GCTCAGC              GC^T[ACGT]AGC
              638  GCTCAGC             
Eam1105I     2427  GACTTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2461  GACACTTGGTC         
Eco81I       2187  CCTGAGG              CC^T[ACGT]AGG
             2741  CCTCAGG             
EcoNI         187  CCTTGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1829  CCTGAAGCAGG         
HgaI           80  GACGC                GACGC(5/10)
             2305  GCGTC               
Hin1I          13  GGCGCC               G[GA]^CG[CT]C
               80  GACGCC              
HindIII      1521  AAGCTT               A^AGCTT
             2781  AAGCTT              
PflMI        1802  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2549  CCACGCAATGG         
PpuMI        1542  GGGTCCC              [GA]G^G[AT]CC[CT]
             3377  GGGACCT             
RcaI         2002  TCATGA               T^CATGA
             2296  TCATGA              
SmlI         2885  CTTGAG               C^T[CT][GA]AG
             3183  CTCAAG              
Van91I       1802  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2549  CCACGCAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
Asp700I     AspHI       AvaI        AvaII       BanII       
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI