##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AccIII AclI AcsI AgeI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AvrII BbeI BcgI
BciVI BlnI BsaWI BseAI BsiWI
BspDI BspEI BsrDI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI HpaI KasI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3686 GACGTC GACGT^C
Acc65I 1163 GGTACC G^GTACC
AlwNI 2290 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1163 GGTACC G^GTACC
AspI 2113 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3024 CCTCAGC CCTCAGC(-5/-2)
BclI 1430 TGATCA T^GATCA
BglII 1268 AGATCT A^GATCT
BsaI 2672 GAGACC GGTCTC(1/5)
BsmI 1521 GAATGC GAATGC(1/-1)
Bsp1407I 1110 TGTACA T^GTACA
BspHI 1508 TCATGA T^CATGA
BspLU11I 1538 ACATGT A^CATGT
BsrGI 1110 TGTACA T^GTACA
BstAPI 2464 GCAAACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3889 TTCGAA TT^CGAA
DraIII 1571 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 1430 TGATCA T^GATCA
HincII 1306 GTTGAC GT[CT]^[GA]AC
HindII 1306 GTTGAC GT[CT]^[GA]AC
HindIII 953 AAGCTT A^AGCTT
KpnI 1163 GGTACC GGTAC^C
NspV 3889 TTCGAA TT^CGAA
PflFI 2113 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1535 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1508 TCATGA T^CATGA
ScaI 584 AGTACT AGT^ACT
SfiI 3320 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3889 TTCGAA TT^CGAA
SspBI 1110 TGTACA T^GTACA
TfiI 3383 GATTC G^A[AT]TC
Tth111I 2113 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1535 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 2071 CTTAAG C^TTAAG
2578 CTTAAG
Alw44I 751 GTGCAC G^TGCAC
1620 GTGCAC
ApaLI 751 GTGCAC G^TGCAC
1620 GTGCAC
AviII 2 TGCGCA TGC^GCA
2697 TGCGCA
BbsI 3695 GAAGAC GAAGAC(2/6)
2401 GTCTTC
BfrI 2071 CTTAAG C^TTAAG
2578 CTTAAG
BpuAI 3695 GAAGAC GAAGAC(2/6)
2401 GTCTTC
BsaBI 972 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1693 GATTGGGATC
BsmBI 1720 GAGACG CGTCTC(1/5)
3346 GAGACG
BssHII 3100 GCGCGC G^CGCGC
3102 GCGCGC
BstXI 559 CCAAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1042 CCAGACTTATGG
EagI 20 CGGCCG C^GGCCG
101 CGGCCG
EclXI 20 CGGCCG C^GGCCG
101 CGGCCG
Eco52I 20 CGGCCG C^GGCCG
101 CGGCCG
EcoNI 618 CCTTGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1377 CCTGCAGCAGG
FspI 2 TGCGCA TGC^GCA
2697 TGCGCA
MamI 972 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1693 GATTGGGATC
NaeI 99 GCCGGC GCC^GGC
1237 GCCGGC
NgoMIV 99 GCCGGC G^CCGGC
1237 GCCGGC
PaeR7I 2838 CTCGAG C^TCGAG
3139 CTCGAG
StuI 681 AGGCCT AGG^CCT
698 AGGCCT
XhoI 2838 CTCGAG C^TCGAG
3139 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AocI Aor51HI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII