##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AgeI Alw44I Aor51HI ApaLI AscI
AvrII BbeI BbvCI BciVI BlnI
BlpI Bpu1102I BsiWI BspDI BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
KasI KspI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SacII SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2535 GACGTC GACGT^C
AclI 357 AACGTT AA^CGTT
AflII 819 CTTAAG C^TTAAG
ApaI 1242 GGGCCC GGGCC^C
AseI 786 ATTAAT AT^TAAT
AsnI 786 ATTAAT AT^TAAT
AspI 2066 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1790 TGCGCA TGC^GCA
BalI 1966 TGGCCA TGG^CCA
BamHI 2518 GGATCC G^GATCC
BcgI 1540 GCAACTTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1837 TGATCA T^GATCA
BfrI 819 CTTAAG C^TTAAG
BglI 1959 GCCTCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1547 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1529 CGACCG CG[GA][CT]^CG
BsmI 2306 GCATTC GAATGC(1/-1)
Bsp1407I 2777 TGTACA T^GTACA
BspHI 1118 TCATGA T^CATGA
BspLU11I 1231 ACATGT A^CATGT
BspMI 2267 ACCTGC ACCTGC(4/8)
BsrGI 2777 TGTACA T^GTACA
BssHII 133 GCGCGC G^CGCGC
BssSI 1878 CTCGTG CACGAG(-5/-1)
BstXI 253 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2987 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 707 GAATTC G^AATTC
EcoT22I 2110 ATGCAT ATGCA^T
FbaI 1837 TGATCA T^GATCA
FspI 1790 TGCGCA TGC^GCA
MamI 1547 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 414 CAATTG C^AATTG
MluI 3462 ACGCGT A^CGCGT
MluNI 1966 TGGCCA TGG^CCA
MscI 1966 TGGCCA TGG^CCA
MunI 414 CAATTG C^AATTG
NdeI 1088 CATATG CA^TATG
NsiI 2110 ATGCAT ATGCA^T
PflFI 2066 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2172 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 786 ATTAAT AT^TAAT
Psp1406I 357 AACGTT AA^CGTT
RcaI 1118 TCATGA T^CATGA
SalI 2973 GTCGAC G^TCGAC
SmaI 2010 CCCGGG CCC^GGG
SphI 1690 GCATGC GCATG^C
SspBI 2777 TGTACA T^GTACA
SspI 3585 AATATT AAT^ATT
Tth111I 2066 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2172 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 523 CCATCTCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2010 CCCGGG C^CCGGG
XmaI 2010 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 350 GGTACC G^GTACC
1744 GGTACC
AccI 1908 GTCTAC GT^[AC][GT]AC
2973 GTCGAC
AccIII 985 TCCGGA T^CCGGA
2608 TCCGGA
AcyI 2535 GACGTC G[GA]^CG[CT]C
3408 GACGCC
AflIII 1231 ACATGT A^C[GA][CT]GT
3462 ACGCGT
AocI 2080 CCTCAGG CC^T[ACGT]AGG
2103 CCTCAGG
Asp700I 1229 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1262 GAAGCGGTTC
Asp718I 350 GGTACC G^GTACC
1744 GGTACC
BbrPI 2189 CACGTG CAC^GTG
3480 CACGTG
BglII 590 AGATCT A^GATCT
859 AGATCT
BsaAI 2189 CACGTG [CT]AC^GT[GA]
3480 CACGTG
BsaHI 2535 GACGTC G[GA]^CG[CT]C
3408 GACGCC
BsaI 1021 GAGACC GGTCTC(1/5)
3621 GAGACC
BsaWI 985 TCCGGA [AT]^CCGG[AT]
2608 TCCGGA
BseAI 985 TCCGGA T^CCGGA
2608 TCCGGA
BsmBI 472 GAGACG CGTCTC(1/5)
2886 GAGACG
BspEI 985 TCCGGA T^CCGGA
2608 TCCGGA
BsrBI 1194 GAGCGG CCGCTC(-3/-3)
1306 GAGCGG
BsrFI 2864 GCCGGT [GA]^CCGG[CT]
3394 GCCGGT
Bsu36I 2080 CCTCAGG CC^T[ACGT]AGG
2103 CCTCAGG
Cfr10I 2864 GCCGGT [GA]^CCGG[CT]
3394 GCCGGT
DraI 1179 TTTAAA TTT^AAA
2529 TTTAAA
EaeI 1966 TGGCCA [CT]^GGCC[GA]
3647 TGGCCG
Eco81I 2080 CCTCAGG CC^T[ACGT]AGG
2103 CCTCAGG
HaeII 79 GGCGCT [GA]GCGC^[CT]
1625 AGCGCC
Hin1I 2535 GACGTC G[GA]^CG[CT]C
3408 GACGCC
HindIII 2757 AAGCTT A^AGCTT
3520 AAGCTT
HpaI 780 GTTAAC GTT^AAC
2571 GTTAAC
KpnI 350 GGTACC GGTAC^C
1744 GGTACC
MroI 985 TCCGGA T^CCGGA
2608 TCCGGA
NcoI 1855 CCATGG C^CATGG
2968 CCATGG
PmaCI 2189 CACGTG CAC^GTG
3480 CACGTG
PmlI 2189 CACGTG CAC^GTG
3480 CACGTG
SacI 1727 GAGCTC GAGCT^C
3014 GAGCTC
XmnI 1229 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1262 GAAGCGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AspEI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII