##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
ApaI AseI AsnI Asp718I AspEI
AviII BamHI BbeI BbrPI BbvCI
BclI BfrI BglI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspMI BssSI BstBI
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI KpnI MamI MroI MslI
NaeI NarI NgoMIV NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
StuI SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 432 GACGTC GACGT^C
AclI 1172 AACGTT AA^CGTT
Alw44I 2264 GTGCAC G^TGCAC
Aor51HI 2223 AGCGCT AGC^GCT
ApaLI 2264 GTGCAC G^TGCAC
AscI 208 GGCGCGCC GG^CGCGCC
Asp700I 354 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 311 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 880 TGGCCA TGG^CCA
BanI 2107 GGTGCC G^G[CT][GA]CC
BcgI 1133 GCAGAGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3087 GGATAC GTATCC(6/5)
BglII 776 AGATCT A^GATCT
BsaI 912 GAGACC GGTCTC(1/5)
BsgI 770 CTGCAC GTGCAG(16/14)
BsiEI 452 CGACCG CG[GA][CT]^CG
BsmI 1647 GAATGC GAATGC(1/-1)
Bsp1407I 2952 TGTACA T^GTACA
BspLU11I 2897 ACATGT A^CATGT
BsrBI 2168 GAGCGG CCGCTC(-3/-3)
BsrDI 1092 GCAATG GCAATG(2/0)
BsrFI 9 ACCGGC [GA]^CCGG[CT]
BsrGI 2952 TGTACA T^GTACA
Bst1107I 2245 GTATAC GTA^TAC
BstAPI 2123 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 260 GGTGACC G^GT[ACGT]ACC
BstPI 260 GGTGACC G^GT[ACGT]ACC
BstZ17I 2245 GTATAC GTA^TAC
Cfr10I 9 ACCGGC [GA]^CCGG[CT]
EaeI 880 TGGCCA [CT]^GGCC[GA]
Eco47III 2223 AGCGCT AGC^GCT
EcoO65I 260 GGTGACC G^GT[ACGT]ACC
HaeII 2223 AGCGCT [GA]GCGC^[CT]
HincII 1160 GTCAAC GT[CT]^[GA]AC
HindII 1160 GTCAAC GT[CT]^[GA]AC
HindIII 2319 AAGCTT A^AGCTT
KspI 288 CCGCGG CCGC^GG
MfeI 2789 CAATTG C^AATTG
MluI 601 ACGCGT A^CGCGT
MluNI 880 TGGCCA TGG^CCA
MscI 880 TGGCCA TGG^CCA
MunI 2789 CAATTG C^AATTG
NcoI 2080 CCATGG C^CATGG
NdeI 2975 CATATG CA^TATG
NheI 2422 GCTAGC G^CTAGC
NruI 1616 TCGCGA TCG^CGA
NspI 2897 ACATGT [GA]CATG^[CT]
PflFI 311 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1172 AACGTT AA^CGTT
SacI 1625 GAGCTC GAGCT^C
SacII 288 CCGCGG CCGC^GG
SapI 2002 GAAGAGC GCTCTTC(1/4)
SfcI 2456 CTATAG C^T[GA][CT]AG
SpeI 2712 ACTAGT A^CTAGT
SspBI 2952 TGTACA T^GTACA
Tth111I 311 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 354 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 67 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1020 CAGTTCCTG
AocI 64 CCTCAGG CC^T[ACGT]AGG
1518 CCTCAGG
AvaI 130 CCCGGG C^[CT]CG[GA]G
266 CCCGGG
AvrII 1997 CCTAGG C^CTAGG
2118 CCTAGG
BanII 1428 GGGCTC G[GA]GC[CT]^C
1625 GAGCTC
BlnI 1997 CCTAGG C^CTAGG
2118 CCTAGG
BsaAI 1320 TACGTG [CT]AC^GT[GA]
2510 CACGTA
BsoBI 130 CCCGGG C^[CT]CG[GA]G
266 CCCGGG
Bsu36I 64 CCTCAGG CC^T[ACGT]AGG
1518 CCTCAGG
DraIII 1678 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2124 CACAAAGTG
EarI 1702 GAAGAG CTCTTC(1/4)
2002 GAAGAG
Eco81I 64 CCTCAGG CC^T[ACGT]AGG
1518 CCTCAGG
EcoRI 354 GAATTC G^AATTC
575 GAATTC
Ksp632I 1702 GAAGAG CTCTTC(1/4)
2002 GAAGAG
PpuMI 1513 GGGACCC [GA]G^G[AT]CC[CT]
2563 GGGTCCT
SmaI 130 CCCGGG CCC^GGG
266 CCCGGG
SspI 2909 AATATT AAT^ATT
3034 AATATT
TaqI 640 TCGA T^CGA
2603 TCGA
TthHB8I 640 TCGA T^CGA
2603 TCGA
XbaI 2292 TCTAGA T^CTAGA
2593 TCTAGA
XmaCI 130 CCCGGG C^CCGGG
266 CCCGGG
XmaI 130 CCCGGG C^CCGGG
266 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AvaII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII