################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccIII AflII AgeI AhdI ApaI AseI AsnI Asp718I AspEI AviII BamHI BbeI BbrPI BbvCI BclI BfrI BglI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BspMI BssSI BstBI CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI MamI MroI MslI NaeI NarI NgoMIV NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I StuI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 432 GACGTC GACGT^C AclI 1172 AACGTT AA^CGTT Alw44I 2264 GTGCAC G^TGCAC Aor51HI 2223 AGCGCT AGC^GCT ApaLI 2264 GTGCAC G^TGCAC AscI 208 GGCGCGCC GG^CGCGCC Asp700I 354 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 311 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 880 TGGCCA TGG^CCA BanI 2107 GGTGCC G^G[CT][GA]CC BcgI 1133 GCAGAGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3087 GGATAC GTATCC(6/5) BglII 776 AGATCT A^GATCT BsaI 912 GAGACC GGTCTC(1/5) BsgI 770 CTGCAC GTGCAG(16/14) BsiEI 452 CGACCG CG[GA][CT]^CG BsmI 1647 GAATGC GAATGC(1/-1) Bsp1407I 2952 TGTACA T^GTACA BspLU11I 2897 ACATGT A^CATGT BsrBI 2168 GAGCGG CCGCTC(-3/-3) BsrDI 1092 GCAATG GCAATG(2/0) BsrFI 9 ACCGGC [GA]^CCGG[CT] BsrGI 2952 TGTACA T^GTACA Bst1107I 2245 GTATAC GTA^TAC BstAPI 2123 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 260 GGTGACC G^GT[ACGT]ACC BstPI 260 GGTGACC G^GT[ACGT]ACC BstZ17I 2245 GTATAC GTA^TAC Cfr10I 9 ACCGGC [GA]^CCGG[CT] EaeI 880 TGGCCA [CT]^GGCC[GA] Eco47III 2223 AGCGCT AGC^GCT EcoO65I 260 GGTGACC G^GT[ACGT]ACC HaeII 2223 AGCGCT [GA]GCGC^[CT] HincII 1160 GTCAAC GT[CT]^[GA]AC HindII 1160 GTCAAC GT[CT]^[GA]AC HindIII 2319 AAGCTT A^AGCTT KspI 288 CCGCGG CCGC^GG MfeI 2789 CAATTG C^AATTG MluI 601 ACGCGT A^CGCGT MluNI 880 TGGCCA TGG^CCA MscI 880 TGGCCA TGG^CCA MunI 2789 CAATTG C^AATTG NcoI 2080 CCATGG C^CATGG NdeI 2975 CATATG CA^TATG NheI 2422 GCTAGC G^CTAGC NruI 1616 TCGCGA TCG^CGA NspI 2897 ACATGT [GA]CATG^[CT] PflFI 311 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 1172 AACGTT AA^CGTT SacI 1625 GAGCTC GAGCT^C SacII 288 CCGCGG CCGC^GG SapI 2002 GAAGAGC GCTCTTC(1/4) SfcI 2456 CTATAG C^T[GA][CT]AG SpeI 2712 ACTAGT A^CTAGT SspBI 2952 TGTACA T^GTACA Tth111I 311 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 354 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 67 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1020 CAGTTCCTG AocI 64 CCTCAGG CC^T[ACGT]AGG 1518 CCTCAGG AvaI 130 CCCGGG C^[CT]CG[GA]G 266 CCCGGG AvrII 1997 CCTAGG C^CTAGG 2118 CCTAGG BanII 1428 GGGCTC G[GA]GC[CT]^C 1625 GAGCTC BlnI 1997 CCTAGG C^CTAGG 2118 CCTAGG BsaAI 1320 TACGTG [CT]AC^GT[GA] 2510 CACGTA BsoBI 130 CCCGGG C^[CT]CG[GA]G 266 CCCGGG Bsu36I 64 CCTCAGG CC^T[ACGT]AGG 1518 CCTCAGG DraIII 1678 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2124 CACAAAGTG EarI 1702 GAAGAG CTCTTC(1/4) 2002 GAAGAG Eco81I 64 CCTCAGG CC^T[ACGT]AGG 1518 CCTCAGG EcoRI 354 GAATTC G^AATTC 575 GAATTC Ksp632I 1702 GAAGAG CTCTTC(1/4) 2002 GAAGAG PpuMI 1513 GGGACCC [GA]G^G[AT]CC[CT] 2563 GGGTCCT SmaI 130 CCCGGG CCC^GGG 266 CCCGGG SspI 2909 AATATT AAT^ATT 3034 AATATT TaqI 640 TCGA T^CGA 2603 TCGA TthHB8I 640 TCGA T^CGA 2603 TCGA XbaI 2292 TCTAGA T^CTAGA 2593 TCTAGA XmaCI 130 CCCGGG C^CCGGG 266 CCCGGG XmaI 130 CCCGGG C^CCGGG 266 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AspHI AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII