##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BamHI BbrPI BciVI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BsrDI BssHII BstEII BstPI Bsu36I
ClaI CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoRV FseI FspI HincII
HindII HpaI KpnI KspI MamI
MluI MroI NdeI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SgrAI SpeI
SspI SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AlwI 1878 GGATC GGATC(4/5)
AvrII 2522 CCTAGG C^CTAGG
BalI 572 TGGCCA TGG^CCA
BbeI 92 GGCGCC GGCGC^C
BcgI 81 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3154 TGATCA T^GATCA
BglII 925 AGATCT A^GATCT
BlnI 2522 CCTAGG C^CTAGG
BsaI 850 GAGACC GGTCTC(1/5)
BspHI 509 TCATGA T^CATGA
BspLU11I 2596 ACATGT A^CATGT
BssSI 3338 CACGAG CACGAG(-5/-1)
Bst1107I 2600 GTATAC GTA^TAC
BstBI 1302 TTCGAA TT^CGAA
BstXI 605 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2600 GTATAC GTA^TAC
DrdI 3191 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 647 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 165 GAATTC G^AATTC
EcoT22I 3373 ATGCAT ATGCA^T
FbaI 3154 TGATCA T^GATCA
HindIII 1934 AAGCTT A^AGCTT
KasI 92 GGCGCC G^GCGCC
MfeI 3301 CAATTG C^AATTG
MluNI 572 TGGCCA TGG^CCA
MscI 572 TGGCCA TGG^CCA
MunI 3301 CAATTG C^AATTG
NaeI 3267 GCCGGC GCC^GGC
NarI 92 GGCGCC GG^CGCC
NcoI 553 CCATGG C^CATGG
NgoMIV 3267 GCCGGC G^CCGGC
NsiI 3373 ATGCAT ATGCA^T
NspV 1302 TTCGAA TT^CGAA
PflMI 548 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 509 TCATGA T^CATGA
SbfI 1512 CCTGCAGG CCTGCA^GG
ScaI 3550 AGTACT AGT^ACT
SexAI 1315 ACCAGGT A^CC[AT]GGT
SfiI 551 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 92 GGCGCC GGC^GCC
SfuI 1302 TTCGAA TT^CGAA
SnaBI 2565 TACGTA TAC^GTA
SphI 1133 GCATGC GCATG^C
Sse8387I 1512 CCTGCAGG CCTGCA^GG
Van91I 548 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 312 GTCTAC GT^[AC][GT]AC
2600 GTATAC
AccII 1721 CGCG CG^CG
2623 CGCG
AcsI 165 GAATTC [GA]^AATT[CT]
1340 GAATTT
AflII 1019 CTTAAG C^TTAAG
2358 CTTAAG
ApoI 165 GAATTC [GA]^AATT[CT]
1340 GAATTT
BfrI 1019 CTTAAG C^TTAAG
2358 CTTAAG
BsmI 1541 GAATGC GAATGC(1/-1)
3375 GCATTC
BsrFI 775 ACCGGC [GA]^CCGG[CT]
3267 GCCGGC
BstUI 1721 CGCG CG^CG
2623 CGCG
BstYI 925 AGATCT [GA]^GATC[CT]
1878 GGATCT
Cfr10I 775 ACCGGC [GA]^CCGG[CT]
3267 GCCGGC
MflI 925 AGATCT [GA]^GATC[CT]
1878 GGATCT
MvnI 1721 CGCG CG^CG
2623 CGCG
NheI 3069 GCTAGC G^CTAGC
3291 GCTAGC
PshAI 774 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2936 GACTCAGGTC
TaqI 54 TCGA T^CGA
1303 TCGA
TthHB8I 54 TCGA T^CGA
1303 TCGA
XhoII 925 AGATCT [GA]^GATC[CT]
1878 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwNI ApaI Asp700I AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrGI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I XmaCI XmaI XmnI