##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaHI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoT22I FbaI FseI Hin1I
HpaI KasI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 1627 AAATTT [GA]^AATT[CT]
Aor51HI 1538 AGCGCT AGC^GCT
ApaI 1257 GGGCCC GGGCC^C
ApoI 1627 AAATTT [GA]^AATT[CT]
AspHI 923 GAGCAC G[AT]GC[AT]^C
AspI 1829 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1817 TGCGCA TGC^GCA
BbsI 202 GAAGAC GAAGAC(2/6)
BglII 245 AGATCT A^GATCT
BpuAI 202 GAAGAC GAAGAC(2/6)
BsaAI 437 CACGTA [CT]AC^GT[GA]
BsaBI 1579 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2030 GAGACC GGTCTC(1/5)
BsiHKAI 923 GAGCAC G[AT]GC[AT]^C
BsmBI 130 GAGACG CGTCTC(1/5)
BspHI 1772 TCATGA T^CATGA
BspMI 1272 GCAGGT ACCTGC(4/8)
BsrDI 2010 CATTGC GCAATG(2/0)
BssHII 1602 GCGCGC G^CGCGC
BstEII 1861 GGTGACC G^GT[ACGT]ACC
BstPI 1861 GGTGACC G^GT[ACGT]ACC
DsaI 174 CCGCGG C^C[GA][CT]GG
Eco47III 1538 AGCGCT AGC^GCT
EcoO65I 1861 GGTGACC G^GT[ACGT]ACC
EcoRV 1558 GATATC GAT^ATC
FspI 1817 TGCGCA TGC^GCA
HgaI 6 GACGC GACGC(5/10)
HincII 2494 GTTGAC GT[CT]^[GA]AC
HindII 2494 GTTGAC GT[CT]^[GA]AC
HindIII 1735 AAGCTT A^AGCTT
KspI 174 CCGCGG CCGC^GG
MamI 1579 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1154 CAATTG C^AATTG
MunI 1154 CAATTG C^AATTG
NruI 64 TCGCGA TCG^CGA
PflFI 1829 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1136 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1772 TCATGA T^CATGA
SacII 174 CCGCGG CCGC^GG
SapI 1660 GAAGAGC GCTCTTC(1/4)
SmaI 2117 CCCGGG CCC^GGG
TaqI 1652 TCGA T^CGA
Tth111I 1829 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1652 TCGA T^CGA
Van91I 1136 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2476 TCTAGA T^CTAGA
XcmI 2301 CCAGGCCTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2117 CCCGGG C^CCGGG
XmaI 2117 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Acc65I 613 GGTACC G^GTACC
1275 GGTACC
Asp718I 613 GGTACC G^GTACC
1275 GGTACC
AvaI 1454 CCCGAG C^[CT]CG[GA]G
2117 CCCGGG
BamHI 1343 GGATCC G^GATCC
2246 GGATCC
BsaWI 153 ACCGGA [AT]^CCGG[AT]
888 TCCGGT
BseRI 1410 CTCCTC GAGGAG(10/8)
1744 CTCCTC
BsmI 399 GAATGC GAATGC(1/-1)
2280 GAATGC
BsoBI 1454 CCCGAG C^[CT]CG[GA]G
2117 CCCGGG
BsrBI 1968 CCGCTC CCGCTC(-3/-3)
2286 CCGCTC
BssSI 1421 CTCGTG CACGAG(-5/-1)
2036 CTCGTG
BstAPI 361 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
436 GCACGTAATGC
HaeII 208 AGCGCC [GA]GCGC^[CT]
1538 AGCGCT
KpnI 613 GGTACC GGTAC^C
1275 GGTACC
PstI 2066 CTGCAG CTGCA^G
2099 CTGCAG
SmlI 58 CTTGAG C^T[CT][GA]AG
1244 CTCAAG
SphI 1606 GCATGC GCATG^C
2508 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII