################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AvrII BbeI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FbaI FseI Hin1I HpaI KasI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 1627 AAATTT [GA]^AATT[CT] Aor51HI 1538 AGCGCT AGC^GCT ApaI 1257 GGGCCC GGGCC^C ApoI 1627 AAATTT [GA]^AATT[CT] AspHI 923 GAGCAC G[AT]GC[AT]^C AspI 1829 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1817 TGCGCA TGC^GCA BbsI 202 GAAGAC GAAGAC(2/6) BglII 245 AGATCT A^GATCT BpuAI 202 GAAGAC GAAGAC(2/6) BsaAI 437 CACGTA [CT]AC^GT[GA] BsaBI 1579 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2030 GAGACC GGTCTC(1/5) BsiHKAI 923 GAGCAC G[AT]GC[AT]^C BsmBI 130 GAGACG CGTCTC(1/5) BspHI 1772 TCATGA T^CATGA BspMI 1272 GCAGGT ACCTGC(4/8) BsrDI 2010 CATTGC GCAATG(2/0) BssHII 1602 GCGCGC G^CGCGC BstEII 1861 GGTGACC G^GT[ACGT]ACC BstPI 1861 GGTGACC G^GT[ACGT]ACC DsaI 174 CCGCGG C^C[GA][CT]GG Eco47III 1538 AGCGCT AGC^GCT EcoO65I 1861 GGTGACC G^GT[ACGT]ACC EcoRV 1558 GATATC GAT^ATC FspI 1817 TGCGCA TGC^GCA HgaI 6 GACGC GACGC(5/10) HincII 2494 GTTGAC GT[CT]^[GA]AC HindII 2494 GTTGAC GT[CT]^[GA]AC HindIII 1735 AAGCTT A^AGCTT KspI 174 CCGCGG CCGC^GG MamI 1579 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1154 CAATTG C^AATTG MunI 1154 CAATTG C^AATTG NruI 64 TCGCGA TCG^CGA PflFI 1829 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1136 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1772 TCATGA T^CATGA SacII 174 CCGCGG CCGC^GG SapI 1660 GAAGAGC GCTCTTC(1/4) SmaI 2117 CCCGGG CCC^GGG TaqI 1652 TCGA T^CGA Tth111I 1829 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1652 TCGA T^CGA Van91I 1136 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2476 TCTAGA T^CTAGA XcmI 2301 CCAGGCCTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2117 CCCGGG C^CCGGG XmaI 2117 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Acc65I 613 GGTACC G^GTACC 1275 GGTACC Asp718I 613 GGTACC G^GTACC 1275 GGTACC AvaI 1454 CCCGAG C^[CT]CG[GA]G 2117 CCCGGG BamHI 1343 GGATCC G^GATCC 2246 GGATCC BsaWI 153 ACCGGA [AT]^CCGG[AT] 888 TCCGGT BseRI 1410 CTCCTC GAGGAG(10/8) 1744 CTCCTC BsmI 399 GAATGC GAATGC(1/-1) 2280 GAATGC BsoBI 1454 CCCGAG C^[CT]CG[GA]G 2117 CCCGGG BsrBI 1968 CCGCTC CCGCTC(-3/-3) 2286 CCGCTC BssSI 1421 CTCGTG CACGAG(-5/-1) 2036 CTCGTG BstAPI 361 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 436 GCACGTAATGC HaeII 208 AGCGCC [GA]GCGC^[CT] 1538 AGCGCT KpnI 613 GGTACC GGTAC^C 1275 GGTACC PstI 2066 CTGCAG CTGCA^G 2099 CTGCAG SmlI 58 CTTGAG C^T[CT][GA]AG 1244 CTCAAG SphI 1606 GCATGC GCATG^C 2508 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII