##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbeI BcgI BciVI
BfrI BlnI BsaBI BsaI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraIII
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KasI MamI MfeI MroI MunI
NarI NcoI NdeI NheI NotI
NsiI NspV PacI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SexAI SfoI SfuI
SgrAI SmaI SnaBI SphI SspBI
SspI SwaI Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1129 GGTACC G^GTACC
AcsI 2318 GAATTT [GA]^AATT[CT]
AcyI 324 GACGCC G[GA]^CG[CT]C
ApoI 2318 GAATTT [GA]^AATT[CT]
Asp718I 1129 GGTACC G^GTACC
AspI 578 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2630 TGCGCA TGC^GCA
BalI 2493 TGGCCA TGG^CCA
BbrPI 1548 CACGTG CAC^GTG
BclI 1531 TGATCA T^GATCA
BsaAI 1548 CACGTG [CT]AC^GT[GA]
BsaHI 324 GACGCC G[GA]^CG[CT]C
BsmI 2275 GCATTC GAATGC(1/-1)
BspLU11I 2307 ACATGT A^CATGT
BsrBI 678 GAGCGG CCGCTC(-3/-3)
BstAPI 2426 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2812 GGTGACC G^GT[ACGT]ACC
BstPI 2812 GGTGACC G^GT[ACGT]ACC
DraI 2327 TTTAAA TTT^AAA
DrdI 2175 GACTCTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 35 CCTCGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2812 GGTGACC G^GT[ACGT]ACC
FbaI 1531 TGATCA T^GATCA
FspI 2630 TGCGCA TGC^GCA
HaeII 547 AGCGCC [GA]GCGC^[CT]
Hin1I 324 GACGCC G[GA]^CG[CT]C
KpnI 1129 GGTACC GGTAC^C
KspI 614 CCGCGG CCGC^GG
MluI 154 ACGCGT A^CGCGT
MluNI 2493 TGGCCA TGG^CCA
MscI 2493 TGGCCA TGG^CCA
NruI 943 TCGCGA TCG^CGA
PaeR7I 2687 CTCGAG C^TCGAG
PflFI 578 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1548 CACGTG CAC^GTG
PmlI 1548 CACGTG CAC^GTG
PshAI 1553 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 614 CCGCGG CCGC^GG
SapI 341 GCTCTTC GCTCTTC(1/4)
SbfI 2571 CCTGCAGG CCTGCA^GG
ScaI 3023 AGTACT AGT^ACT
SfiI 612 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2921 ACTAGT A^CTAGT
Sse8387I 2571 CCTGCAGG CCTGCA^GG
StuI 643 AGGCCT AGG^CCT
Tsp509I 2319 AATT ^AATT
Tth111I 578 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2687 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 154 ACGCGT A^C[GA][CT]GT
2307 ACATGT
AocI 2707 CCTAAGG CC^T[ACGT]AGG
2888 CCTAAGG
ApaI 27 GGGCCC GGGCC^C
188 GGGCCC
BanI 1129 GGTACC G^G[CT][GA]CC
2398 GGCACC
BbsI 1223 GAAGAC GAAGAC(2/6)
1365 GAAGAC
BbvCI 233 CCTCAGC CCTCAGC(-5/-2)
2781 CCTCAGC
BglII 1807 AGATCT A^GATCT
2003 AGATCT
BlpI 407 GCTCAGC GC^T[ACGT]AGC
483 GCTGAGC
Bpu1102I 407 GCTCAGC GC^T[ACGT]AGC
483 GCTGAGC
BpuAI 1223 GAAGAC GAAGAC(2/6)
1365 GAAGAC
BsiEI 620 CGGCCG CG[GA][CT]^CG
993 CGGCCG
BsrI 2077 ACTGG ACTGG(1/-1)
3080 CCAGT
BssSI 1053 CTCGTG CACGAG(-5/-1)
2941 CTCGTG
Bsu36I 2707 CCTAAGG CC^T[ACGT]AGG
2888 CCTAAGG
CelII 407 GCTCAGC GC^T[ACGT]AGC
483 GCTGAGC
DsaI 614 CCGCGG C^C[GA][CT]GG
2440 CCACGG
EagI 620 CGGCCG C^GGCCG
993 CGGCCG
EclXI 620 CGGCCG C^GGCCG
993 CGGCCG
Eco52I 620 CGGCCG C^GGCCG
993 CGGCCG
Eco81I 2707 CCTAAGG CC^T[ACGT]AGG
2888 CCTAAGG
MaeII 1189 ACGT A^CGT
1549 ACGT
MslI 2441 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2611 CATCTCTGTG
NaeI 634 GCCGGC GCC^GGC
1395 GCCGGC
NgoMIV 634 GCCGGC G^CCGGC
1395 GCCGGC
XcmI 959 CCAGGCTGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2933 CCAGGCTCCTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII