##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccI AccIII AcsI AgeI
AlwI ApoI AscI AseI AsnI
Asp700I AvrII BamHI BbeI BcgI
BlnI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI KspI MamI
MfeI MroI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SspI SwaI Tsp509I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2361 GGTACC G^GTACC
AclI 1320 AACGTT AA^CGTT
AcyI 2859 GGCGTC G[GA]^CG[CT]C
AflII 1976 CTTAAG C^TTAAG
AhdI 2734 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1588 GTGCAC G^TGCAC
Aor51HI 1870 AGCGCT AGC^GCT
ApaLI 1588 GTGCAC G^TGCAC
Asp718I 2361 GGTACC G^GTACC
AspEI 2734 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2539 TGCGCA TGC^GCA
BbrPI 2097 CACGTG CAC^GTG
BbvCI 846 CCTCAGC CCTCAGC(-5/-2)
BciVI 1333 GTATCC GTATCC(6/5)
BclI 1454 TGATCA T^GATCA
BfrI 1976 CTTAAG C^TTAAG
BsaHI 2859 GGCGTC G[GA]^CG[CT]C
BsaWI 2845 TCCGGT [AT]^CCGG[AT]
BsmI 1178 GAATGC GAATGC(1/-1)
BspHI 2686 TCATGA T^CATGA
BspLU11I 1136 ACATGT A^CATGT
BsrBI 811 CCGCTC CCGCTC(-3/-3)
BsrFI 1669 GCCGGC [GA]^CCGG[CT]
BstBI 153 TTCGAA TT^CGAA
Cfr10I 1669 GCCGGC [GA]^CCGG[CT]
DraIII 1982 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2195 GACACTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2734 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1870 AGCGCT AGC^GCT
FbaI 1454 TGATCA T^GATCA
FspI 2539 TGCGCA TGC^GCA
Hin1I 2859 GGCGTC G[GA]^CG[CT]C
HincII 219 GTCAAC GT[CT]^[GA]AC
HindII 219 GTCAAC GT[CT]^[GA]AC
KpnI 2361 GGTACC GGTAC^C
MluI 2223 ACGCGT A^CGCGT
MseI 1977 TTAA T^TAA
NaeI 1669 GCCGGC GCC^GGC
NcoI 274 CCATGG C^CATGG
NgoMIV 1669 GCCGGC G^CCGGC
NspV 153 TTCGAA TT^CGAA
PflFI 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 792 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2097 CACGTG CAC^GTG
PmlI 2097 CACGTG CAC^GTG
Psp1406I 1320 AACGTT AA^CGTT
RcaI 2686 TCATGA T^CATGA
SapI 1494 GCTCTTC GCTCTTC(1/4)
SbfI 198 CCTGCAGG CCTGCA^GG
SexAI 962 ACCTGGT A^CC[AT]GGT
SfuI 153 TTCGAA TT^CGAA
Sse8387I 198 CCTGCAGG CCTGCA^GG
Tru9I 1977 TTAA T^TAA
Tth111I 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 792 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 740 CCTCAGG CC^T[ACGT]AGG
2596 CCTCAGG
ApaI 774 GGGCCC GGGCC^C
1300 GGGCCC
BanI 2213 GGCACC G^G[CT][GA]CC
2361 GGTACC
BglI 1302 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1523 GCCCTCCAGGC
BglII 17 AGATCT A^GATCT
1552 AGATCT
BsaAI 2097 CACGTG [CT]AC^GT[GA]
2656 CACGTA
Bsp1407I 2320 TGTACA T^GTACA
2887 TGTACA
BsrGI 2320 TGTACA T^GTACA
2887 TGTACA
BstXI 268 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1270 CCAACTGCCTGG
BstYI 17 AGATCT [GA]^GATC[CT]
1552 AGATCT
Bsu36I 740 CCTCAGG CC^T[ACGT]AGG
2596 CCTCAGG
Eco81I 740 CCTCAGG CC^T[ACGT]AGG
2596 CCTCAGG
EcoT14I 274 CCATGG C^C[AT][AT]GG
1367 CCAAGG
HaeII 1870 AGCGCT [GA]GCGC^[CT]
2671 AGCGCC
MflI 17 AGATCT [GA]^GATC[CT]
1552 AGATCT
SacI 63 GAGCTC GAGCT^C
336 GAGCTC
SmaI 979 CCCGGG CCC^GGG
1583 CCCGGG
SmlI 897 CTCAAG C^T[CT][GA]AG
1976 CTTAAG
SphI 1151 GCATGC GCATG^C
1570 GCATGC
SspBI 2320 TGTACA T^GTACA
2887 TGTACA
StyI 274 CCATGG C^C[AT][AT]GG
1367 CCAAGG
XbaI 602 TCTAGA T^CTAGA
2255 TCTAGA
XcmI 101 CCAGGCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
289 CCAGGTTGGACGTGG
XhoII 17 AGATCT [GA]^GATC[CT]
1552 AGATCT
XmaCI 979 CCCGGG C^CCGGG
1583 CCCGGG
XmaI 979 CCCGGG C^CCGGG
1583 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AvaI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I