################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccI AccIII AcsI AgeI AlwI ApoI AscI AseI AsnI Asp700I AvrII BamHI BbeI BcgI BlnI BsaBI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KspI MamI MfeI MroI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI ScaI SfiI SfoI SgrAI SnaBI SpeI SspI SwaI Tsp509I XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 2361 GGTACC G^GTACC AclI 1320 AACGTT AA^CGTT AcyI 2859 GGCGTC G[GA]^CG[CT]C AflII 1976 CTTAAG C^TTAAG AhdI 2734 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1588 GTGCAC G^TGCAC Aor51HI 1870 AGCGCT AGC^GCT ApaLI 1588 GTGCAC G^TGCAC Asp718I 2361 GGTACC G^GTACC AspEI 2734 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2539 TGCGCA TGC^GCA BbrPI 2097 CACGTG CAC^GTG BbvCI 846 CCTCAGC CCTCAGC(-5/-2) BciVI 1333 GTATCC GTATCC(6/5) BclI 1454 TGATCA T^GATCA BfrI 1976 CTTAAG C^TTAAG BsaHI 2859 GGCGTC G[GA]^CG[CT]C BsaWI 2845 TCCGGT [AT]^CCGG[AT] BsmI 1178 GAATGC GAATGC(1/-1) BspHI 2686 TCATGA T^CATGA BspLU11I 1136 ACATGT A^CATGT BsrBI 811 CCGCTC CCGCTC(-3/-3) BsrFI 1669 GCCGGC [GA]^CCGG[CT] BstBI 153 TTCGAA TT^CGAA Cfr10I 1669 GCCGGC [GA]^CCGG[CT] DraIII 1982 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2195 GACACTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2734 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1870 AGCGCT AGC^GCT FbaI 1454 TGATCA T^GATCA FspI 2539 TGCGCA TGC^GCA Hin1I 2859 GGCGTC G[GA]^CG[CT]C HincII 219 GTCAAC GT[CT]^[GA]AC HindII 219 GTCAAC GT[CT]^[GA]AC KpnI 2361 GGTACC GGTAC^C MluI 2223 ACGCGT A^CGCGT MseI 1977 TTAA T^TAA NaeI 1669 GCCGGC GCC^GGC NcoI 274 CCATGG C^CATGG NgoMIV 1669 GCCGGC G^CCGGC NspV 153 TTCGAA TT^CGAA PflFI 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 792 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2097 CACGTG CAC^GTG PmlI 2097 CACGTG CAC^GTG Psp1406I 1320 AACGTT AA^CGTT RcaI 2686 TCATGA T^CATGA SapI 1494 GCTCTTC GCTCTTC(1/4) SbfI 198 CCTGCAGG CCTGCA^GG SexAI 962 ACCTGGT A^CC[AT]GGT SfuI 153 TTCGAA TT^CGAA Sse8387I 198 CCTGCAGG CCTGCA^GG Tru9I 1977 TTAA T^TAA Tth111I 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 792 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AocI 740 CCTCAGG CC^T[ACGT]AGG 2596 CCTCAGG ApaI 774 GGGCCC GGGCC^C 1300 GGGCCC BanI 2213 GGCACC G^G[CT][GA]CC 2361 GGTACC BglI 1302 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1523 GCCCTCCAGGC BglII 17 AGATCT A^GATCT 1552 AGATCT BsaAI 2097 CACGTG [CT]AC^GT[GA] 2656 CACGTA Bsp1407I 2320 TGTACA T^GTACA 2887 TGTACA BsrGI 2320 TGTACA T^GTACA 2887 TGTACA BstXI 268 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1270 CCAACTGCCTGG BstYI 17 AGATCT [GA]^GATC[CT] 1552 AGATCT Bsu36I 740 CCTCAGG CC^T[ACGT]AGG 2596 CCTCAGG Eco81I 740 CCTCAGG CC^T[ACGT]AGG 2596 CCTCAGG EcoT14I 274 CCATGG C^C[AT][AT]GG 1367 CCAAGG HaeII 1870 AGCGCT [GA]GCGC^[CT] 2671 AGCGCC MflI 17 AGATCT [GA]^GATC[CT] 1552 AGATCT SacI 63 GAGCTC GAGCT^C 336 GAGCTC SmaI 979 CCCGGG CCC^GGG 1583 CCCGGG SmlI 897 CTCAAG C^T[CT][GA]AG 1976 CTTAAG SphI 1151 GCATGC GCATG^C 1570 GCATGC SspBI 2320 TGTACA T^GTACA 2887 TGTACA StyI 274 CCATGG C^C[AT][AT]GG 1367 CCAAGG XbaI 602 TCTAGA T^CTAGA 2255 TCTAGA XcmI 101 CCAGGCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 289 CCAGGTTGGACGTGG XhoII 17 AGATCT [GA]^GATC[CT] 1552 AGATCT XmaCI 979 CCCGGG C^CCGGG 1583 CCCGGG XmaI 979 CCCGGG C^CCGGG 1583 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwNI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TaqI TfiI TseI Tsp45I TspRI TthHB8I