##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AocI AscI Asp700I Asp718I AvaI
BbrPI BcgI BciVI BfrI BsaBI
BsiEI BsiWI BsoBI BspLU11I BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I Cfr10I CpoI DrdI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV FseI HgaI HindIII
KpnI KspI MamI MluI NaeI
NdeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SfiI SgrAI SmaI SnaBI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2534 TCCGGA T^CCGGA
AhdI 1286 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4120 ATTAAT AT^TAAT
AsnI 4120 ATTAAT AT^TAAT
AspEI 1286 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 591 TGCGCA TGC^GCA
BamHI 1299 GGATCC G^GATCC
BclI 3940 TGATCA T^GATCA
BglII 3537 AGATCT A^GATCT
BsaAI 2833 CACGTA [CT]AC^GT[GA]
BsaWI 2534 TCCGGA [AT]^CCGG[AT]
BseAI 2534 TCCGGA T^CCGGA
BsmBI 2002 GAGACG CGTCTC(1/5)
BsmI 1517 GAATGC GAATGC(1/-1)
Bsp1407I 468 TGTACA T^GTACA
BspDI 686 ATCGAT AT^CGAT
BspEI 2534 TCCGGA T^CCGGA
BspHI 3937 TCATGA T^CATGA
BsrBI 3765 CCGCTC CCGCTC(-3/-3)
BsrGI 468 TGTACA T^GTACA
BstBI 704 TTCGAA TT^CGAA
ClaI 686 ATCGAT AT^CGAT
DraI 3993 TTTAAA TTT^AAA
Eam1105I 1286 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2171 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3940 TGATCA T^GATCA
FspI 591 TGCGCA TGC^GCA
MroI 2534 TCCGGA T^CCGGA
NheI 3586 GCTAGC G^CTAGC
NspV 704 TTCGAA TT^CGAA
PshBI 4120 ATTAAT AT^TAAT
RcaI 3937 TCATGA T^CATGA
SbfI 865 CCTGCAGG CCTGCA^GG
ScaI 816 AGTACT AGT^ACT
SexAI 804 ACCTGGT A^CC[AT]GGT
SfuI 704 TTCGAA TT^CGAA
SpeI 3544 ACTAGT A^CTAGT
SphI 2713 GCATGC GCATG^C
Sse8387I 865 CCTGCAGG CCTGCA^GG
SspBI 468 TGTACA T^GTACA
SspI 60 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 860 GACGTC GACGT^C
2004 GACGTC
AccI 536 GTAGAC GT^[AC][GT]AC
872 GTCTAC
AccII 401 CGCG CG^CG
3385 CGCG
AspI 1084 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1717 GACTGAGTC
AvrII 218 CCTAGG C^CTAGG
3610 CCTAGG
BbeI 3057 GGCGCC GGCGC^C
3135 GGCGCC
BlnI 218 CCTAGG C^CTAGG
3610 CCTAGG
BlpI 3680 GCTGAGC GC^T[ACGT]AGC
3753 GCTGAGC
Bpu1102I 3680 GCTGAGC GC^T[ACGT]AGC
3753 GCTGAGC
BsaI 2882 GGTCTC GGTCTC(1/5)
3633 GGTCTC
BstUI 401 CGCG CG^CG
3385 CGCG
CelII 3680 GCTGAGC GC^T[ACGT]AGC
3753 GCTGAGC
HpaI 153 GTTAAC GTT^AAC
3548 GTTAAC
KasI 3057 GGCGCC G^GCGCC
3135 GGCGCC
MfeI 268 CAATTG C^AATTG
2212 CAATTG
MunI 268 CAATTG C^AATTG
2212 CAATTG
MvnI 401 CGCG CG^CG
3385 CGCG
NarI 3057 GGCGCC GG^CGCC
3135 GGCGCC
NspI 2713 GCATGC [GA]CATG^[CT]
3178 GCATGT
PflFI 1084 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1717 GACTGAGTC
SacI 2118 GAGCTC GAGCT^C
3756 GAGCTC
SapI 1336 GCTCTTC GCTCTTC(1/4)
1650 GCTCTTC
SfoI 3057 GGCGCC GGC^GCC
3135 GGCGCC
Tth111I 1084 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1717 GACTGAGTC
XcmI 303 CCATCCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3852 CCAGACTGGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII