##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII BamHI BbeI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BsaHI BsaI BsaWI BseAI
BseRI BsiEI BspDI BspEI BspHI
BspLU11I BsrFI BssHII BstXI CfoI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MfeI
MluI MroI MspI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 2703 CGCG CG^CG
AflII 2584 CTTAAG C^TTAAG
AflIII 584 ACGTGT A^C[GA][CT]GT
AocI 1259 CCTGAGG CC^T[ACGT]AGG
AvaI 2616 CTCGGG C^[CT]CG[GA]G
AvrII 669 CCTAGG C^CTAGG
BbrPI 1276 CACGTG CAC^GTG
BbsI 1107 GTCTTC GAAGAC(2/6)
BfrI 2584 CTTAAG C^TTAAG
BlnI 669 CCTAGG C^CTAGG
BpuAI 1107 GTCTTC GAAGAC(2/6)
BsaBI 1131 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 153 CGTACG C^GTACG
BsmI 675 GAATGC GAATGC(1/-1)
BsoBI 2616 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2546 TGTACA T^GTACA
BspMI 1550 GCAGGT ACCTGC(4/8)
BsrBI 459 GAGCGG CCGCTC(-3/-3)
BsrGI 2546 TGTACA T^GTACA
Bst1107I 2066 GTATAC GTA^TAC
BstBI 836 TTCGAA TT^CGAA
BstUI 2703 CGCG CG^CG
BstZ17I 2066 GTATAC GTA^TAC
Bsu36I 1259 CCTGAGG CC^T[ACGT]AGG
DraI 1701 TTTAAA TTT^AAA
Eco81I 1259 CCTGAGG CC^T[ACGT]AGG
EcoNI 2583 CCTTAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 677 ATGCAT ATGCA^T
FauI 2704 GCGGG CCCGC(4/6)
HgaI 1212 GCGTC GACGC(5/10)
HincII 1738 GTCAAC GT[CT]^[GA]AC
HindII 1738 GTCAAC GT[CT]^[GA]AC
HindIII 2491 AAGCTT A^AGCTT
MamI 1131 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2703 CGCG CG^CG
NsiI 677 ATGCAT ATGCA^T
NspV 836 TTCGAA TT^CGAA
PflMI 419 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1276 CACGTG CAC^GTG
PmlI 1276 CACGTG CAC^GTG
PpuMI 2756 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 1486 GAGCTC GAGCT^C
SapI 2746 GCTCTTC GCTCTTC(1/4)
SfuI 836 TTCGAA TT^CGAA
SphI 2158 GCATGC GCATG^C
SspBI 2546 TGTACA T^GTACA
SspI 1871 AATATT AAT^ATT
Van91I 419 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 390 GTCTAC GT^[AC][GT]AC
2066 GTATAC
BalI 133 TGGCCA TGG^CCA
1460 TGGCCA
BanII 1486 GAGCTC G[GA]GC[CT]^C
2620 GGGCTC
BsaAI 906 TACGTG [CT]AC^GT[GA]
1276 CACGTG
BsmBI 1213 CGTCTC CGTCTC(1/5)
928 GAGACG
BstAPI 2230 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2737 GCACTGGCTGC
BstYI 352 GGATCT [GA]^GATC[CT]
2110 AGATCC
DraII 421 AGGCCCT [GA]G^G[ACGT]CC[CT]
2756 AGGTCCC
EaeI 133 TGGCCA [CT]^GGCC[GA]
1460 TGGCCA
EarI 2433 CTCTTC CTCTTC(1/4)
2747 CTCTTC
EcoO109I 421 AGGCCCT [GA]G^G[ACGT]CC[CT]
2756 AGGTCCC
EcoRI 810 GAATTC G^AATTC
1901 GAATTC
Ksp632I 2433 CTCTTC CTCTTC(1/4)
2747 CTCTTC
MflI 352 GGATCT [GA]^GATC[CT]
2110 AGATCC
MluNI 133 TGGCCA TGG^CCA
1460 TGGCCA
MscI 133 TGGCCA TGG^CCA
1460 TGGCCA
PstI 1545 CTGCAG CTGCA^G
2447 CTGCAG
ScaI 1849 AGTACT AGT^ACT
2451 AGTACT
XcmI 531 CCAGATTTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2774 CCAGAATCCACATGG
XhoII 352 GGATCT [GA]^GATC[CT]
2110 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI Cac8I
CelII Cfr13I DdeI DpnII DsaI
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I