################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII BamHI BbeI BbvCI BcgI BciVI BclI BcnI BglI BglII BsaHI BsaI BsaWI BseAI BseRI BsiEI BspDI BspEI BspHI BspLU11I BsrFI BssHII BstXI CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MfeI MluI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 2703 CGCG CG^CG AflII 2584 CTTAAG C^TTAAG AflIII 584 ACGTGT A^C[GA][CT]GT AocI 1259 CCTGAGG CC^T[ACGT]AGG AvaI 2616 CTCGGG C^[CT]CG[GA]G AvrII 669 CCTAGG C^CTAGG BbrPI 1276 CACGTG CAC^GTG BbsI 1107 GTCTTC GAAGAC(2/6) BfrI 2584 CTTAAG C^TTAAG BlnI 669 CCTAGG C^CTAGG BpuAI 1107 GTCTTC GAAGAC(2/6) BsaBI 1131 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 153 CGTACG C^GTACG BsmI 675 GAATGC GAATGC(1/-1) BsoBI 2616 CTCGGG C^[CT]CG[GA]G Bsp1407I 2546 TGTACA T^GTACA BspMI 1550 GCAGGT ACCTGC(4/8) BsrBI 459 GAGCGG CCGCTC(-3/-3) BsrGI 2546 TGTACA T^GTACA Bst1107I 2066 GTATAC GTA^TAC BstBI 836 TTCGAA TT^CGAA BstUI 2703 CGCG CG^CG BstZ17I 2066 GTATAC GTA^TAC Bsu36I 1259 CCTGAGG CC^T[ACGT]AGG DraI 1701 TTTAAA TTT^AAA Eco81I 1259 CCTGAGG CC^T[ACGT]AGG EcoNI 2583 CCTTAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 677 ATGCAT ATGCA^T FauI 2704 GCGGG CCCGC(4/6) HgaI 1212 GCGTC GACGC(5/10) HincII 1738 GTCAAC GT[CT]^[GA]AC HindII 1738 GTCAAC GT[CT]^[GA]AC HindIII 2491 AAGCTT A^AGCTT MamI 1131 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 2703 CGCG CG^CG NsiI 677 ATGCAT ATGCA^T NspV 836 TTCGAA TT^CGAA PflMI 419 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1276 CACGTG CAC^GTG PmlI 1276 CACGTG CAC^GTG PpuMI 2756 AGGTCCC [GA]G^G[AT]CC[CT] SacI 1486 GAGCTC GAGCT^C SapI 2746 GCTCTTC GCTCTTC(1/4) SfuI 836 TTCGAA TT^CGAA SphI 2158 GCATGC GCATG^C SspBI 2546 TGTACA T^GTACA SspI 1871 AATATT AAT^ATT Van91I 419 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 390 GTCTAC GT^[AC][GT]AC 2066 GTATAC BalI 133 TGGCCA TGG^CCA 1460 TGGCCA BanII 1486 GAGCTC G[GA]GC[CT]^C 2620 GGGCTC BsaAI 906 TACGTG [CT]AC^GT[GA] 1276 CACGTG BsmBI 1213 CGTCTC CGTCTC(1/5) 928 GAGACG BstAPI 2230 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2737 GCACTGGCTGC BstYI 352 GGATCT [GA]^GATC[CT] 2110 AGATCC DraII 421 AGGCCCT [GA]G^G[ACGT]CC[CT] 2756 AGGTCCC EaeI 133 TGGCCA [CT]^GGCC[GA] 1460 TGGCCA EarI 2433 CTCTTC CTCTTC(1/4) 2747 CTCTTC EcoO109I 421 AGGCCCT [GA]G^G[ACGT]CC[CT] 2756 AGGTCCC EcoRI 810 GAATTC G^AATTC 1901 GAATTC Ksp632I 2433 CTCTTC CTCTTC(1/4) 2747 CTCTTC MflI 352 GGATCT [GA]^GATC[CT] 2110 AGATCC MluNI 133 TGGCCA TGG^CCA 1460 TGGCCA MscI 133 TGGCCA TGG^CCA 1460 TGGCCA PstI 1545 CTGCAG CTGCA^G 2447 CTGCAG ScaI 1849 AGTACT AGT^ACT 2451 AGTACT XcmI 531 CCAGATTTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2774 CCAGAATCCACATGG XhoII 352 GGATCT [GA]^GATC[CT] 2110 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanI BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI Cac8I CelII Cfr13I DdeI DpnII DsaI EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I