################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AclI AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AvrII BbrPI BciVI BclI BglII BlnI BsaAI BsiWI Bsp1407I BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CpoI DraI DraIII Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KpnI MluI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 425 GACGTC GACGT^C AcsI 3056 GAATTT [GA]^AATT[CT] AlwNI 1159 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 3056 GAATTT [GA]^AATT[CT] Asp700I 2853 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 620 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1296 TGCGCA TGC^GCA BalI 66 TGGCCA TGG^CCA BbsI 485 GTCTTC GAAGAC(2/6) BpuAI 485 GTCTTC GAAGAC(2/6) BsaBI 1196 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1637 GAGACG CGTCTC(1/5) BsmI 507 GCATTC GAATGC(1/-1) BspDI 1152 ATCGAT AT^CGAT ClaI 1152 ATCGAT AT^CGAT DrdI 604 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 1296 TGCGCA TGC^GCA HgaI 1642 GACGC GACGC(5/10) MamI 1196 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2816 CAATTG C^AATTG MluNI 66 TGGCCA TGG^CCA MscI 66 TGGCCA TGG^CCA MunI 2816 CAATTG C^AATTG NotI 2220 GCGGCCGC GC^GGCCGC NspI 1103 GCATGT [GA]CATG^[CT] PflFI 620 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1136 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1192 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 666 CAGCTG CAG^CTG SapI 712 GCTCTTC GCTCTTC(1/4) Tth111I 620 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1136 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2853 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 239 GTCTAC GT^[AC][GT]AC 1067 GTCTAC AccIII 2548 TCCGGA T^CCGGA 2809 TCCGGA AflII 374 CTTAAG C^TTAAG 2535 CTTAAG AhdI 1255 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2243 GACGTGAAGTC AspEI 1255 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2243 GACGTGAAGTC BamHI 739 GGATCC G^GATCC 1601 GGATCC BfaI 539 CTAG C^TAG 786 CTAG BfrI 374 CTTAAG C^TTAAG 2535 CTTAAG BlpI 2897 GCTGAGC GC^T[ACGT]AGC 2952 GCTCAGC Bpu1102I 2897 GCTGAGC GC^T[ACGT]AGC 2952 GCTCAGC BsaI 1454 GAGACC GGTCTC(1/5) 1397 GGTCTC BseAI 2548 TCCGGA T^CCGGA 2809 TCCGGA BspEI 2548 TCCGGA T^CCGGA 2809 TCCGGA BssHII 2023 GCGCGC G^CGCGC 2598 GCGCGC BstYI 739 GGATCC [GA]^GATC[CT] 1601 GGATCC CelII 2897 GCTGAGC GC^T[ACGT]AGC 2952 GCTCAGC Eam1105I 1255 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2243 GACGTGAAGTC EcoNI 630 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2862 CCTGAAGGAGG EcoT14I 348 CCTTGG C^C[AT][AT]GG 1536 CCAAGG FseI 2201 GGCCGGCC GGCCGG^CC 2578 GGCCGGCC MaeI 539 CTAG C^TAG 786 CTAG MflI 739 GGATCC [GA]^GATC[CT] 1601 GGATCC MroI 2548 TCCGGA T^CCGGA 2809 TCCGGA MslI 1664 CACCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2134 CACGCGAGTG NaeI 2202 GCCGGC GCC^GGC 2579 GCCGGC NgoMIV 2202 GCCGGC G^CCGGC 2579 GCCGGC SexAI 1183 ACCTGGT A^CC[AT]GGT 2882 ACCTGGT SfiI 1354 GGCCTGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2213 GGCCATCGCGGCC StyI 348 CCTTGG C^C[AT][AT]GG 1536 CCAAGG TfiI 1220 GAATC G^A[AT]TC 1280 GATTC Tsp509I 2817 AATT ^AATT 3057 AATT XcmI 1136 CCATACTGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2910 CCAGGAGAGCAGTGG XhoII 739 GGATCC [GA]^GATC[CT] 1601 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvCI BbvI BcgI BcnI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoO65I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI TaqI Tru9I TseI Tsp45I TspRI TthHB8I XmaCI XmaI