##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AclI AflIII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AvrII BbrPI BciVI
BclI BglII BlnI BsaAI BsiWI
Bsp1407I BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CpoI DraI DraIII
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI MluI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 425 GACGTC GACGT^C
AcsI 3056 GAATTT [GA]^AATT[CT]
AlwNI 1159 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 3056 GAATTT [GA]^AATT[CT]
Asp700I 2853 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 620 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1296 TGCGCA TGC^GCA
BalI 66 TGGCCA TGG^CCA
BbsI 485 GTCTTC GAAGAC(2/6)
BpuAI 485 GTCTTC GAAGAC(2/6)
BsaBI 1196 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1637 GAGACG CGTCTC(1/5)
BsmI 507 GCATTC GAATGC(1/-1)
BspDI 1152 ATCGAT AT^CGAT
ClaI 1152 ATCGAT AT^CGAT
DrdI 604 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1296 TGCGCA TGC^GCA
HgaI 1642 GACGC GACGC(5/10)
MamI 1196 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2816 CAATTG C^AATTG
MluNI 66 TGGCCA TGG^CCA
MscI 66 TGGCCA TGG^CCA
MunI 2816 CAATTG C^AATTG
NotI 2220 GCGGCCGC GC^GGCCGC
NspI 1103 GCATGT [GA]CATG^[CT]
PflFI 620 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1136 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1192 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 666 CAGCTG CAG^CTG
SapI 712 GCTCTTC GCTCTTC(1/4)
Tth111I 620 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1136 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2853 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 239 GTCTAC GT^[AC][GT]AC
1067 GTCTAC
AccIII 2548 TCCGGA T^CCGGA
2809 TCCGGA
AflII 374 CTTAAG C^TTAAG
2535 CTTAAG
AhdI 1255 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2243 GACGTGAAGTC
AspEI 1255 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2243 GACGTGAAGTC
BamHI 739 GGATCC G^GATCC
1601 GGATCC
BfaI 539 CTAG C^TAG
786 CTAG
BfrI 374 CTTAAG C^TTAAG
2535 CTTAAG
BlpI 2897 GCTGAGC GC^T[ACGT]AGC
2952 GCTCAGC
Bpu1102I 2897 GCTGAGC GC^T[ACGT]AGC
2952 GCTCAGC
BsaI 1454 GAGACC GGTCTC(1/5)
1397 GGTCTC
BseAI 2548 TCCGGA T^CCGGA
2809 TCCGGA
BspEI 2548 TCCGGA T^CCGGA
2809 TCCGGA
BssHII 2023 GCGCGC G^CGCGC
2598 GCGCGC
BstYI 739 GGATCC [GA]^GATC[CT]
1601 GGATCC
CelII 2897 GCTGAGC GC^T[ACGT]AGC
2952 GCTCAGC
Eam1105I 1255 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2243 GACGTGAAGTC
EcoNI 630 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2862 CCTGAAGGAGG
EcoT14I 348 CCTTGG C^C[AT][AT]GG
1536 CCAAGG
FseI 2201 GGCCGGCC GGCCGG^CC
2578 GGCCGGCC
MaeI 539 CTAG C^TAG
786 CTAG
MflI 739 GGATCC [GA]^GATC[CT]
1601 GGATCC
MroI 2548 TCCGGA T^CCGGA
2809 TCCGGA
MslI 1664 CACCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2134 CACGCGAGTG
NaeI 2202 GCCGGC GCC^GGC
2579 GCCGGC
NgoMIV 2202 GCCGGC G^CCGGC
2579 GCCGGC
SexAI 1183 ACCTGGT A^CC[AT]GGT
2882 ACCTGGT
SfiI 1354 GGCCTGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2213 GGCCATCGCGGCC
StyI 348 CCTTGG C^C[AT][AT]GG
1536 CCAAGG
TfiI 1220 GAATC G^A[AT]TC
1280 GATTC
Tsp509I 2817 AATT ^AATT
3057 AATT
XcmI 1136 CCATACTGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2910 CCAGGAGAGCAGTGG
XhoII 739 GGATCC [GA]^GATC[CT]
1601 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvCI BbvI
BcgI BcnI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoO65I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XmaCI XmaI