##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       Alw44I      
AlwNI       ApaLI       AscI        Asp700I     Asp718I     
BamHI       BfrI        BglI        BsaBI       BsiEI       
BsmBI       BspDI       BspHI       BspLU11I    BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco52I      EcoRI       EcoRV       EcoT22I     FseI        
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NaeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PmeI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
Van91I      XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        263  TCCGGA               T^CCGGA
AcsI         2670  AAATTC               [GA]^AATT[CT]
AflIII        736  ACGTGT               A^C[GA][CT]GT
AgeI         1404  ACCGGT               A^CCGGT
Aor51HI      3246  AGCGCT               AGC^GCT
ApoI         2670  AAATTC               [GA]^AATT[CT]
AseI         1560  ATTAAT               AT^TAAT
AsnI         1560  ATTAAT               AT^TAAT
AvaI          727  CCCGGG               C^[CT]CG[GA]G
AvrII        2862  CCTAGG               C^CTAGG
BbeI           61  GGCGCC               GGCGC^C
BbvCI        3132  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1135  GTATCC               GTATCC(6/5)
BclI         1399  TGATCA               T^GATCA
BlnI         2862  CCTAGG               C^CTAGG
BseAI         263  TCCGGA               T^CCGGA
BsiWI        1684  CGTACG               C^GTACG
BsmI         2287  GCATTC               GAATGC(1/-1)
BsoBI         727  CCCGGG               C^[CT]CG[GA]G
BspEI         263  TCCGGA               T^CCGGA
BsrDI        1451  CATTGC               GCAATG(2/0)
BssSI        1812  CACGAG               CACGAG(-5/-1)
DraIII        549  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     3246  AGCGCT               AGC^GCT
FbaI         1399  TGATCA               T^GATCA
KasI           61  GGCGCC               G^GCGCC
MroI          263  TCCGGA               T^CCGGA
NarI           61  GGCGCC               GG^CGCC
NdeI         2137  CATATG               CA^TATG
PinAI        1404  ACCGGT               A^CCGGT
PshAI         249  GACTACCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1560  ATTAAT               AT^TAAT
PstI          777  CTGCAG               CTGCA^G
SapI         2507  GCTCTTC              GCTCTTC(1/4)
SfoI           61  GGCGCC               GGC^GCC
SmaI          727  CCCGGG               CCC^GGG
SmlI          540  CTCAAG               C^T[CT][GA]AG
SspI         2628  AATATT               AAT^ATT
XbaI         2911  TCTAGA               T^CTAGA
XmaCI         727  CCCGGG               C^CCGGG
XmaI          727  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccI          276  GTCTAC               GT^[AC][GT]AC
             1950  GTAGAC              
AcyI           61  GGCGCC               G[GA]^CG[CT]C
              117  GACGCC              
AhdI          326  GACAGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2933  GACCCTCTGTC         
AocI         2157  CCTCAGG              CC^T[ACGT]AGG
             2258  CCTGAGG             
AspEI         326  GACAGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2933  GACCCTCTGTC         
AspI         2376  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3122  GACCCTGTC           
BbrPI         627  CACGTG               CAC^GTG
              735  CACGTG              
BcgI         2333  CGAGGCCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1541  GCACAAGGTTCG        
BglII         781  AGATCT               A^GATCT
             1438  AGATCT              
BsaHI          61  GGCGCC               G[GA]^CG[CT]C
              117  GACGCC              
BsgI          953  CTGCAC               GTGCAG(16/14)
             1880  CTGCAC              
Bsp1407I     2248  TGTACA               T^GTACA
             3195  TGTACA              
BsrBI         351  GAGCGG               CCGCTC(-3/-3)
              444  GAGCGG              
BsrGI        2248  TGTACA               T^GTACA
             3195  TGTACA              
BstEII        944  GGTGACC              G^GT[ACGT]ACC
             2763  GGTCACC             
BstPI         944  GGTGACC              G^GT[ACGT]ACC
             2763  GGTCACC             
BstXI         934  CCAGTGGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1076  CCAGTCTGCTGG        
Bsu36I       2157  CCTCAGG              CC^T[ACGT]AGG
             2258  CCTGAGG             
Eam1105I      326  GACAGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2933  GACCCTCTGTC         
Eco81I       2157  CCTCAGG              CC^T[ACGT]AGG
             2258  CCTGAGG             
EcoNI        2153  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2258  CCTGAGGAAGG         
EcoO65I       944  GGTGACC              G^GT[ACGT]ACC
             2763  GGTCACC             
Hin1I          61  GGCGCC               G[GA]^CG[CT]C
              117  GACGCC              
HincII       2096  GTTGAC               GT[CT]^[GA]AC
             2382  GTCAAC              
HindII       2096  GTTGAC               GT[CT]^[GA]AC
             2382  GTCAAC              
NspI          400  GCATGC               [GA]CATG^[CT]
             2347  GCATGC              
PflFI        2376  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3122  GACCCTGTC           
PmaCI         627  CACGTG               CAC^GTG
              735  CACGTG              
PmlI          627  CACGTG               CAC^GTG
              735  CACGTG              
PpuMI         535  AGGACCT              [GA]G^G[AT]CC[CT]
             1579  GGGACCC             
SacI          267  GAGCTC               GAGCT^C
             1374  GAGCTC              
SphI          400  GCATGC               GCATG^C
             2347  GCATGC              
SspBI        2248  TGTACA               T^GTACA
             3195  TGTACA              
StuI          389  AGGCCT               AGG^CCT
             1490  AGGCCT              
TfiI          527  GAATC                G^A[AT]TC
             1040  GAATC               
Tth111I      2376  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3122  GACCCTGTC           
XcmI         1318  CCACTATTATCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2317  CCATGATCCGCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaII       AviII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaI        BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII