##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AflII Alw44I
AlwNI ApaLI AscI Asp700I Asp718I
BamHI BfrI BglI BsaBI BsiEI
BsmBI BspDI BspHI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 263 TCCGGA T^CCGGA
AcsI 2670 AAATTC [GA]^AATT[CT]
AflIII 736 ACGTGT A^C[GA][CT]GT
AgeI 1404 ACCGGT A^CCGGT
Aor51HI 3246 AGCGCT AGC^GCT
ApoI 2670 AAATTC [GA]^AATT[CT]
AseI 1560 ATTAAT AT^TAAT
AsnI 1560 ATTAAT AT^TAAT
AvaI 727 CCCGGG C^[CT]CG[GA]G
AvrII 2862 CCTAGG C^CTAGG
BbeI 61 GGCGCC GGCGC^C
BbvCI 3132 GCTGAGG CCTCAGC(-5/-2)
BciVI 1135 GTATCC GTATCC(6/5)
BclI 1399 TGATCA T^GATCA
BlnI 2862 CCTAGG C^CTAGG
BseAI 263 TCCGGA T^CCGGA
BsiWI 1684 CGTACG C^GTACG
BsmI 2287 GCATTC GAATGC(1/-1)
BsoBI 727 CCCGGG C^[CT]CG[GA]G
BspEI 263 TCCGGA T^CCGGA
BsrDI 1451 CATTGC GCAATG(2/0)
BssSI 1812 CACGAG CACGAG(-5/-1)
DraIII 549 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3246 AGCGCT AGC^GCT
FbaI 1399 TGATCA T^GATCA
KasI 61 GGCGCC G^GCGCC
MroI 263 TCCGGA T^CCGGA
NarI 61 GGCGCC GG^CGCC
NdeI 2137 CATATG CA^TATG
PinAI 1404 ACCGGT A^CCGGT
PshAI 249 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1560 ATTAAT AT^TAAT
PstI 777 CTGCAG CTGCA^G
SapI 2507 GCTCTTC GCTCTTC(1/4)
SfoI 61 GGCGCC GGC^GCC
SmaI 727 CCCGGG CCC^GGG
SmlI 540 CTCAAG C^T[CT][GA]AG
SspI 2628 AATATT AAT^ATT
XbaI 2911 TCTAGA T^CTAGA
XmaCI 727 CCCGGG C^CCGGG
XmaI 727 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 276 GTCTAC GT^[AC][GT]AC
1950 GTAGAC
AcyI 61 GGCGCC G[GA]^CG[CT]C
117 GACGCC
AhdI 326 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2933 GACCCTCTGTC
AocI 2157 CCTCAGG CC^T[ACGT]AGG
2258 CCTGAGG
AspEI 326 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2933 GACCCTCTGTC
AspI 2376 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3122 GACCCTGTC
BbrPI 627 CACGTG CAC^GTG
735 CACGTG
BcgI 2333 CGAGGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1541 GCACAAGGTTCG
BglII 781 AGATCT A^GATCT
1438 AGATCT
BsaHI 61 GGCGCC G[GA]^CG[CT]C
117 GACGCC
BsgI 953 CTGCAC GTGCAG(16/14)
1880 CTGCAC
Bsp1407I 2248 TGTACA T^GTACA
3195 TGTACA
BsrBI 351 GAGCGG CCGCTC(-3/-3)
444 GAGCGG
BsrGI 2248 TGTACA T^GTACA
3195 TGTACA
BstEII 944 GGTGACC G^GT[ACGT]ACC
2763 GGTCACC
BstPI 944 GGTGACC G^GT[ACGT]ACC
2763 GGTCACC
BstXI 934 CCAGTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCAGTCTGCTGG
Bsu36I 2157 CCTCAGG CC^T[ACGT]AGG
2258 CCTGAGG
Eam1105I 326 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2933 GACCCTCTGTC
Eco81I 2157 CCTCAGG CC^T[ACGT]AGG
2258 CCTGAGG
EcoNI 2153 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2258 CCTGAGGAAGG
EcoO65I 944 GGTGACC G^GT[ACGT]ACC
2763 GGTCACC
Hin1I 61 GGCGCC G[GA]^CG[CT]C
117 GACGCC
HincII 2096 GTTGAC GT[CT]^[GA]AC
2382 GTCAAC
HindII 2096 GTTGAC GT[CT]^[GA]AC
2382 GTCAAC
NspI 400 GCATGC [GA]CATG^[CT]
2347 GCATGC
PflFI 2376 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3122 GACCCTGTC
PmaCI 627 CACGTG CAC^GTG
735 CACGTG
PmlI 627 CACGTG CAC^GTG
735 CACGTG
PpuMI 535 AGGACCT [GA]G^G[AT]CC[CT]
1579 GGGACCC
SacI 267 GAGCTC GAGCT^C
1374 GAGCTC
SphI 400 GCATGC GCATG^C
2347 GCATGC
SspBI 2248 TGTACA T^GTACA
3195 TGTACA
StuI 389 AGGCCT AGG^CCT
1490 AGGCCT
TfiI 527 GAATC G^A[AT]TC
1040 GAATC
Tth111I 2376 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3122 GACCCTGTC
XcmI 1318 CCACTATTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2317 CCATGATCCGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaII AviII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII