################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AclI AflII Alw44I AlwNI ApaLI AscI Asp700I Asp718I BamHI BfrI BglI BsaBI BsiEI BsmBI BspDI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI KspI MamI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 263 TCCGGA T^CCGGA AcsI 2670 AAATTC [GA]^AATT[CT] AflIII 736 ACGTGT A^C[GA][CT]GT AgeI 1404 ACCGGT A^CCGGT Aor51HI 3246 AGCGCT AGC^GCT ApoI 2670 AAATTC [GA]^AATT[CT] AseI 1560 ATTAAT AT^TAAT AsnI 1560 ATTAAT AT^TAAT AvaI 727 CCCGGG C^[CT]CG[GA]G AvrII 2862 CCTAGG C^CTAGG BbeI 61 GGCGCC GGCGC^C BbvCI 3132 GCTGAGG CCTCAGC(-5/-2) BciVI 1135 GTATCC GTATCC(6/5) BclI 1399 TGATCA T^GATCA BlnI 2862 CCTAGG C^CTAGG BseAI 263 TCCGGA T^CCGGA BsiWI 1684 CGTACG C^GTACG BsmI 2287 GCATTC GAATGC(1/-1) BsoBI 727 CCCGGG C^[CT]CG[GA]G BspEI 263 TCCGGA T^CCGGA BsrDI 1451 CATTGC GCAATG(2/0) BssSI 1812 CACGAG CACGAG(-5/-1) DraIII 549 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 3246 AGCGCT AGC^GCT FbaI 1399 TGATCA T^GATCA KasI 61 GGCGCC G^GCGCC MroI 263 TCCGGA T^CCGGA NarI 61 GGCGCC GG^CGCC NdeI 2137 CATATG CA^TATG PinAI 1404 ACCGGT A^CCGGT PshAI 249 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1560 ATTAAT AT^TAAT PstI 777 CTGCAG CTGCA^G SapI 2507 GCTCTTC GCTCTTC(1/4) SfoI 61 GGCGCC GGC^GCC SmaI 727 CCCGGG CCC^GGG SmlI 540 CTCAAG C^T[CT][GA]AG SspI 2628 AATATT AAT^ATT XbaI 2911 TCTAGA T^CTAGA XmaCI 727 CCCGGG C^CCGGG XmaI 727 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 276 GTCTAC GT^[AC][GT]AC 1950 GTAGAC AcyI 61 GGCGCC G[GA]^CG[CT]C 117 GACGCC AhdI 326 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2933 GACCCTCTGTC AocI 2157 CCTCAGG CC^T[ACGT]AGG 2258 CCTGAGG AspEI 326 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2933 GACCCTCTGTC AspI 2376 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3122 GACCCTGTC BbrPI 627 CACGTG CAC^GTG 735 CACGTG BcgI 2333 CGAGGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1541 GCACAAGGTTCG BglII 781 AGATCT A^GATCT 1438 AGATCT BsaHI 61 GGCGCC G[GA]^CG[CT]C 117 GACGCC BsgI 953 CTGCAC GTGCAG(16/14) 1880 CTGCAC Bsp1407I 2248 TGTACA T^GTACA 3195 TGTACA BsrBI 351 GAGCGG CCGCTC(-3/-3) 444 GAGCGG BsrGI 2248 TGTACA T^GTACA 3195 TGTACA BstEII 944 GGTGACC G^GT[ACGT]ACC 2763 GGTCACC BstPI 944 GGTGACC G^GT[ACGT]ACC 2763 GGTCACC BstXI 934 CCAGTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1076 CCAGTCTGCTGG Bsu36I 2157 CCTCAGG CC^T[ACGT]AGG 2258 CCTGAGG Eam1105I 326 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2933 GACCCTCTGTC Eco81I 2157 CCTCAGG CC^T[ACGT]AGG 2258 CCTGAGG EcoNI 2153 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2258 CCTGAGGAAGG EcoO65I 944 GGTGACC G^GT[ACGT]ACC 2763 GGTCACC Hin1I 61 GGCGCC G[GA]^CG[CT]C 117 GACGCC HincII 2096 GTTGAC GT[CT]^[GA]AC 2382 GTCAAC HindII 2096 GTTGAC GT[CT]^[GA]AC 2382 GTCAAC NspI 400 GCATGC [GA]CATG^[CT] 2347 GCATGC PflFI 2376 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3122 GACCCTGTC PmaCI 627 CACGTG CAC^GTG 735 CACGTG PmlI 627 CACGTG CAC^GTG 735 CACGTG PpuMI 535 AGGACCT [GA]G^G[AT]CC[CT] 1579 GGGACCC SacI 267 GAGCTC GAGCT^C 1374 GAGCTC SphI 400 GCATGC GCATG^C 2347 GCATGC SspBI 2248 TGTACA T^GTACA 3195 TGTACA StuI 389 AGGCCT AGG^CCT 1490 AGGCCT TfiI 527 GAATC G^A[AT]TC 1040 GAATC Tth111I 2376 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3122 GACCCTGTC XcmI 1318 CCACTATTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2317 CCATGATCCGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AspHI AvaII AviII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII