##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AhdI Aor51HI AscI
AseI AsnI Asp700I AspEI AspI
BcgI BclI BsiWI BsmBI BspDI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoRV
FbaI FseI HindIII HpaI MfeI
MluI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PmeI
PshBI Psp1406I RcaI RsrII SalI
SfiI SfuI SgrAI SnaBI SpeI
SwaI Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2264 GGTACC G^GTACC
AflII 2945 CTTAAG C^TTAAG
AgeI 643 ACCGGT A^CCGGT
Alw44I 1597 GTGCAC G^TGCAC
AlwNI 1661 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1597 GTGCAC G^TGCAC
Asp718I 2264 GGTACC G^GTACC
AviII 1081 TGCGCA TGC^GCA
BalI 2288 TGGCCA TGG^CCA
BamHI 365 GGATCC G^GATCC
BbrPI 2278 CACGTG CAC^GTG
BbvCI 954 CCTCAGC CCTCAGC(-5/-2)
BciVI 459 GTATCC GTATCC(6/5)
BfrI 2945 CTTAAG C^TTAAG
BglI 71 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1748 AGATCT A^GATCT
BsaBI 260 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 895 TGTACA T^GTACA
BsrFI 643 ACCGGT [GA]^CCGG[CT]
BsrGI 895 TGTACA T^GTACA
Cfr10I 643 ACCGGT [GA]^CCGG[CT]
DrdI 594 GACCCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 195 CTCTTC CTCTTC(1/4)
EcoNI 2407 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2857 GAATTC G^AATTC
EcoT22I 1801 ATGCAT ATGCA^T
FspI 1081 TGCGCA TGC^GCA
HincII 2684 GTCAAC GT[CT]^[GA]AC
HindII 2684 GTCAAC GT[CT]^[GA]AC
KpnI 2264 GGTACC GGTAC^C
Ksp632I 195 CTCTTC CTCTTC(1/4)
KspI 672 CCGCGG CCGC^GG
MamI 260 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2288 TGGCCA TGG^CCA
MscI 2288 TGGCCA TGG^CCA
MslI 228 CACCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1801 ATGCAT ATGCA^T
PinAI 643 ACCGGT A^CCGGT
PmaCI 2278 CACGTG CAC^GTG
PmlI 2278 CACGTG CAC^GTG
PshAI 642 GACCGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2087 CGATCG CGAT^CG
SacI 2506 GAGCTC GAGCT^C
SacII 672 CCGCGG CCGC^GG
SapI 194 GCTCTTC GCTCTTC(1/4)
SbfI 2487 CCTGCAGG CCTGCA^GG
ScaI 1290 AGTACT AGT^ACT
SexAI 2911 ACCTGGT A^CC[AT]GGT
Sse8387I 2487 CCTGCAGG CCTGCA^GG
SspBI 895 TGTACA T^GTACA
SspI 2529 AATATT AAT^ATT
StuI 2366 AGGCCT AGG^CCT
XbaI 2621 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 869 ACACGT A^C[GA][CT]GT
2476 ACGTGT
AocI 1457 CCTGAGG CC^T[ACGT]AGG
1928 CCTGAGG
AvrII 473 CCTAGG C^CTAGG
2710 CCTAGG
BbsI 1568 GAAGAC GAAGAC(2/6)
2828 GAAGAC
BlnI 473 CCTAGG C^CTAGG
2710 CCTAGG
BpuAI 1568 GAAGAC GAAGAC(2/6)
2828 GAAGAC
BsaAI 2278 CACGTG [CT]AC^GT[GA]
2475 TACGTG
BsaI 489 GAGACC GGTCTC(1/5)
640 GAGACC
BsmI 1799 GAATGC GAATGC(1/-1)
206 GCATTC
BssSI 762 CACGAG CACGAG(-5/-1)
1506 CTCGTG
BstEII 182 GGTCACC G^GT[ACGT]ACC
1352 GGTTACC
BstPI 182 GGTCACC G^GT[ACGT]ACC
1352 GGTTACC
Bsu36I 1457 CCTGAGG CC^T[ACGT]AGG
1928 CCTGAGG
EagI 738 CGGCCG C^GGCCG
773 CGGCCG
EclXI 738 CGGCCG C^GGCCG
773 CGGCCG
Eco52I 738 CGGCCG C^GGCCG
773 CGGCCG
Eco81I 1457 CCTGAGG CC^T[ACGT]AGG
1928 CCTGAGG
EcoO65I 182 GGTCACC G^GT[ACGT]ACC
1352 GGTTACC
PvuII 2049 CAGCTG CAG^CTG
2424 CAGCTG
SmlI 2538 CTTGAG C^T[CT][GA]AG
2945 CTTAAG
TaqI 2086 TCGA T^CGA
3038 TCGA
TfiI 265 GAATC G^A[AT]TC
2513 GATTC
TthHB8I 2086 TCGA T^CGA
3038 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SphI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmaCI XmaI