##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AclI AflII AgeI AocI
AscI AseI AsnI Asp718I AspI
AvaI AvrII BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsiWI BsmI BsoBI
Bsp1407I BsrGI Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII CpoI EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HpaI KpnI
Ksp632I MluI NaeI NcoI NdeI
NgoMIV NheI NotI NsiI PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SfiI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1539 GACGTC GACGT^C
AccI 356 GTCTAC GT^[AC][GT]AC
AcsI 3203 AAATTT [GA]^AATT[CT]
AhdI 767 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2003 GTGCAC G^TGCAC
AlwNI 2131 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3137 AGCGCT AGC^GCT
ApaI 1509 GGGCCC GGGCC^C
ApaLI 2003 GTGCAC G^TGCAC
ApoI 3203 AAATTT [GA]^AATT[CT]
Asp700I 241 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 767 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2006 CACGTG CAC^GTG
BglII 2101 AGATCT A^GATCT
BsaBI 391 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 546 GAGACG CGTCTC(1/5)
BspDI 1271 ATCGAT AT^CGAT
BspHI 1047 TCATGA T^CATGA
BspLU11I 738 ACATGT A^CATGT
BsrDI 146 GCAATG GCAATG(2/0)
BsrFI 3181 GCCGGT [GA]^CCGG[CT]
BstBI 132 TTCGAA TT^CGAA
BstXI 2991 CCAGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3181 GCCGGT [GA]^CCGG[CT]
ClaI 1271 ATCGAT AT^CGAT
DraI 3211 TTTAAA TTT^AAA
Eam1105I 767 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3137 AGCGCT AGC^GCT
HincII 2691 GTCAAC GT[CT]^[GA]AC
HindII 2691 GTCAAC GT[CT]^[GA]AC
HindIII 3019 AAGCTT A^AGCTT
MamI 391 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1852 CAATTG C^AATTG
MslI 628 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1852 CAATTG C^AATTG
NruI 877 TCGCGA TCG^CGA
NspV 132 TTCGAA TT^CGAA
PflMI 770 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2006 CACGTG CAC^GTG
PmlI 2006 CACGTG CAC^GTG
PpuMI 2444 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1047 TCATGA T^CATGA
SbfI 2470 CCTGCAGG CCTGCA^GG
SexAI 840 ACCTGGT A^CC[AT]GGT
SfuI 132 TTCGAA TT^CGAA
Sse8387I 2470 CCTGCAGG CCTGCA^GG
Van91I 770 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 502 CCAGCTGCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 241 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 1374 TCCGGA T^CCGGA
1770 TCCGGA
AflIII 738 ACATGT A^C[GA][CT]GT
990 ACGTGT
BalI 120 TGGCCA TGG^CCA
2988 TGGCCA
BamHI 390 GGATCC G^GATCC
2620 GGATCC
BsaAI 989 TACGTG [CT]AC^GT[GA]
2006 CACGTG
BseAI 1374 TCCGGA T^CCGGA
1770 TCCGGA
BspEI 1374 TCCGGA T^CCGGA
1770 TCCGGA
BssSI 1985 CACGAG CACGAG(-5/-1)
2014 CTCGTG
DrdI 1871 GACGCTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2278 GACCCAGTAGTC
EagI 707 CGGCCG C^GGCCG
1612 CGGCCG
EclXI 707 CGGCCG C^GGCCG
1612 CGGCCG
Eco52I 707 CGGCCG C^GGCCG
1612 CGGCCG
MboII 473 GAAGA GAAGA(8/7)
180 TCTTC
MluNI 120 TGGCCA TGG^CCA
2988 TGGCCA
MroI 1374 TCCGGA T^CCGGA
1770 TCCGGA
MscI 120 TGGCCA TGG^CCA
2988 TGGCCA
NspI 154 ACATGC [GA]CATG^[CT]
738 ACATGT
PshAI 1059 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2809 GACTTCTGTC
PstI 98 CTGCAG CTGCA^G
2471 CTGCAG
SacI 702 GAGCTC GAGCT^C
2887 GAGCTC
StuI 1600 AGGCCT AGG^CCT
3066 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII AviII BanI
BanII BbeI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII