################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AclI AflII AgeI AocI AscI AseI AsnI Asp718I AspI AvaI AvrII BbsI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsiWI BsmI BsoBI Bsp1407I BsrGI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII CpoI EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI Ksp632I MluI NaeI NcoI NdeI NgoMIV NheI NotI NsiI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfiI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1539 GACGTC GACGT^C AccI 356 GTCTAC GT^[AC][GT]AC AcsI 3203 AAATTT [GA]^AATT[CT] AhdI 767 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2003 GTGCAC G^TGCAC AlwNI 2131 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 3137 AGCGCT AGC^GCT ApaI 1509 GGGCCC GGGCC^C ApaLI 2003 GTGCAC G^TGCAC ApoI 3203 AAATTT [GA]^AATT[CT] Asp700I 241 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 767 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 2006 CACGTG CAC^GTG BglII 2101 AGATCT A^GATCT BsaBI 391 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 546 GAGACG CGTCTC(1/5) BspDI 1271 ATCGAT AT^CGAT BspHI 1047 TCATGA T^CATGA BspLU11I 738 ACATGT A^CATGT BsrDI 146 GCAATG GCAATG(2/0) BsrFI 3181 GCCGGT [GA]^CCGG[CT] BstBI 132 TTCGAA TT^CGAA BstXI 2991 CCAGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 3181 GCCGGT [GA]^CCGG[CT] ClaI 1271 ATCGAT AT^CGAT DraI 3211 TTTAAA TTT^AAA Eam1105I 767 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3137 AGCGCT AGC^GCT HincII 2691 GTCAAC GT[CT]^[GA]AC HindII 2691 GTCAAC GT[CT]^[GA]AC HindIII 3019 AAGCTT A^AGCTT MamI 391 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1852 CAATTG C^AATTG MslI 628 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1852 CAATTG C^AATTG NruI 877 TCGCGA TCG^CGA NspV 132 TTCGAA TT^CGAA PflMI 770 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2006 CACGTG CAC^GTG PmlI 2006 CACGTG CAC^GTG PpuMI 2444 AGGACCT [GA]G^G[AT]CC[CT] RcaI 1047 TCATGA T^CATGA SbfI 2470 CCTGCAGG CCTGCA^GG SexAI 840 ACCTGGT A^CC[AT]GGT SfuI 132 TTCGAA TT^CGAA Sse8387I 2470 CCTGCAGG CCTGCA^GG Van91I 770 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 502 CCAGCTGCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 241 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccIII 1374 TCCGGA T^CCGGA 1770 TCCGGA AflIII 738 ACATGT A^C[GA][CT]GT 990 ACGTGT BalI 120 TGGCCA TGG^CCA 2988 TGGCCA BamHI 390 GGATCC G^GATCC 2620 GGATCC BsaAI 989 TACGTG [CT]AC^GT[GA] 2006 CACGTG BseAI 1374 TCCGGA T^CCGGA 1770 TCCGGA BspEI 1374 TCCGGA T^CCGGA 1770 TCCGGA BssSI 1985 CACGAG CACGAG(-5/-1) 2014 CTCGTG DrdI 1871 GACGCTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2278 GACCCAGTAGTC EagI 707 CGGCCG C^GGCCG 1612 CGGCCG EclXI 707 CGGCCG C^GGCCG 1612 CGGCCG Eco52I 707 CGGCCG C^GGCCG 1612 CGGCCG MboII 473 GAAGA GAAGA(8/7) 180 TCTTC MluNI 120 TGGCCA TGG^CCA 2988 TGGCCA MroI 1374 TCCGGA T^CCGGA 1770 TCCGGA MscI 120 TGGCCA TGG^CCA 2988 TGGCCA NspI 154 ACATGC [GA]CATG^[CT] 738 ACATGT PshAI 1059 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2809 GACTTCTGTC PstI 98 CTGCAG CTGCA^G 2471 CTGCAG SacI 702 GAGCTC GAGCT^C 2887 GAGCTC StuI 1600 AGGCCT AGG^CCT 3066 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaII AviII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeII MaeIII MboI MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII