##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AflII AgeI Aor51HI AscI AseI
AsnI Asp700I AspI AviII BamHI
BbeI BbrPI BfrI BsiWI BsmI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HpaI
KasI KspI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SfiI
SfoI SfuI SnaBI SpeI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2584 TCCGGA T^CCGGA
AclI 3339 AACGTT AA^CGTT
AcsI 3712 GAATTT [GA]^AATT[CT]
AflIII 3772 ACATGT A^C[GA][CT]GT
AhdI 3025 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3464 GTGCAC G^TGCAC
ApaI 2934 GGGCCC GGGCC^C
ApaLI 3464 GTGCAC G^TGCAC
ApoI 3712 GAATTT [GA]^AATT[CT]
AspEI 3025 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2201 CCTAGG C^CTAGG
BcgI 3660 GCAGATACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3054 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2201 CCTAGG C^CTAGG
BsaAI 1434 TACGTG [CT]AC^GT[GA]
BsaBI 2081 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2584 TCCGGA [AT]^CCGG[AT]
BseAI 2584 TCCGGA T^CCGGA
BsiEI 3 CGACCG CG[GA][CT]^CG
Bsp1407I 2791 TGTACA T^GTACA
BspDI 2028 ATCGAT AT^CGAT
BspEI 2584 TCCGGA T^CCGGA
BspLU11I 3772 ACATGT A^CATGT
BsrGI 2791 TGTACA T^GTACA
BssSI 2856 CACGAG CACGAG(-5/-1)
BstAPI 1493 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2028 ATCGAT AT^CGAT
Eam1105I 3025 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2978 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 908 AAGCTT A^AGCTT
MamI 2081 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2584 TCCGGA T^CCGGA
NruI 147 TCGCGA TCG^CGA
Psp1406I 3339 AACGTT AA^CGTT
SalI 2234 GTCGAC G^TCGAC
SexAI 3606 ACCTGGT A^CC[AT]GGT
SgrAI 1075 CGCCGGTG C[GA]^CCGG[CT]G
SphI 2195 GCATGC GCATG^C
SspBI 2791 TGTACA T^GTACA
SspI 723 AATATT AAT^ATT
XbaI 199 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 133 GACGTC GACGT^C
2544 GACGTC
Acc65I 1310 GGTACC G^GTACC
2051 GGTACC
AccI 1755 GTCTAC GT^[AC][GT]AC
2234 GTCGAC
AccII 40 CGCG CG^CG
148 CGCG
AocI 3075 CCTGAGG CC^T[ACGT]AGG
3484 CCTCAGG
Asp718I 1310 GGTACC G^GTACC
2051 GGTACC
BbsI 1212 GTCTTC GAAGAC(2/6)
2640 GTCTTC
BbvCI 1065 GCTGAGG CCTCAGC(-5/-2)
3245 GCTGAGG
BciVI 2108 GGATAC GTATCC(6/5)
3405 GTATCC
BglII 865 AGATCT A^GATCT
2248 AGATCT
BpuAI 1212 GTCTTC GAAGAC(2/6)
2640 GTCTTC
BsrBI 1354 CCGCTC CCGCTC(-3/-3)
615 GAGCGG
BsrDI 1219 CATTGC GCAATG(2/0)
2015 CATTGC
BsrFI 1076 GCCGGT [GA]^CCGG[CT]
1747 ACCGGC
BstUI 40 CGCG CG^CG
148 CGCG
Bsu36I 3075 CCTGAGG CC^T[ACGT]AGG
3484 CCTCAGG
Cfr10I 1076 GCCGGT [GA]^CCGG[CT]
1747 ACCGGC
DrdI 2154 GACCACCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3771 GACATGTGTGTC
Eco81I 3075 CCTGAGG CC^T[ACGT]AGG
3484 CCTCAGG
KpnI 1310 GGTACC GGTAC^C
2051 GGTACC
MvnI 40 CGCG CG^CG
148 CGCG
NcoI 1526 CCATGG C^CATGG
2755 CCATGG
SacI 1371 GAGCTC GAGCT^C
2131 GAGCTC
SbfI 1316 CCTGCAGG CCTGCA^GG
2390 CCTGCAGG
SmaI 163 CCCGGG CCC^GGG
513 CCCGGG
Sse8387I 1316 CCTGCAGG CCTGCA^GG
2390 CCTGCAGG
Tsp509I 962 AATT ^AATT
3713 AATT
XmaCI 163 CCCGGG C^CCGGG
513 CCCGGG
XmaI 163 CCCGGG C^CCGGG
513 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII