################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BfrI BglI BlnI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI BstEII BstPI ClaI CpoI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI SspBI SspI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1010 GACGTC GACGT^C AccII 870 CGCG CG^CG AcyI 1010 GACGTC G[GA]^CG[CT]C Alw44I 1823 GTGCAC G^TGCAC Aor51HI 277 AGCGCT AGC^GCT ApaI 2563 GGGCCC GGGCC^C ApaLI 1823 GTGCAC G^TGCAC Asp700I 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 39 CCCGGG C^[CT]CG[GA]G BbvCI 818 CCTCAGC CCTCAGC(-5/-2) BcgI 406 CGAAAGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1183 TGATCA T^GATCA BglII 784 AGATCT A^GATCT BsaAI 950 TACGTG [CT]AC^GT[GA] BsaHI 1010 GACGTC G[GA]^CG[CT]C BsiEI 1 CGGCCG CG[GA][CT]^CG BsmBI 1008 GAGACG CGTCTC(1/5) BsoBI 39 CCCGGG C^[CT]CG[GA]G BspHI 1616 TCATGA T^CATGA BsrBI 1515 GAGCGG CCGCTC(-3/-3) Bst1107I 1423 GTATAC GTA^TAC BstAPI 2742 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 870 CGCG CG^CG BstXI 2438 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1423 GTATAC GTA^TAC DraI 1367 TTTAAA TTT^AAA DraIII 2618 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1 CGGCCG C^GGCCG EclXI 1 CGGCCG C^GGCCG Eco47III 277 AGCGCT AGC^GCT Eco52I 1 CGGCCG C^GGCCG FbaI 1183 TGATCA T^GATCA HgaI 2365 GCGTC GACGC(5/10) Hin1I 1010 GACGTC G[GA]^CG[CT]C MvnI 870 CGCG CG^CG NaeI 497 GCCGGC GCC^GGC NdeI 2086 CATATG CA^TATG NgoMIV 497 GCCGGC G^CCGGC PshAI 3170 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1616 TCATGA T^CATGA SapI 792 GCTCTTC GCTCTTC(1/4) SbfI 2979 CCTGCAGG CCTGCA^GG SmaI 39 CCCGGG CCC^GGG Sse8387I 2979 CCTGCAGG CCTGCA^GG XmaCI 39 CCCGGG C^CCGGG XmaI 39 CCCGGG C^CCGGG XmnI 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 1423 GTATAC GT^[AC][GT]AC 1683 GTAGAC AcsI 518 AAATTT [GA]^AATT[CT] 2168 AAATTC ApoI 518 AAATTT [GA]^AATT[CT] 2168 AAATTC AspI 3022 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3347 GACAAGGTC BsaBI 1178 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2040 GATAGTTATC BstBI 1206 TTCGAA TT^CGAA 2020 TTCGAA HaeII 277 AGCGCT [GA]GCGC^[CT] 822 AGCGCC HindIII 2059 AAGCTT A^AGCTT 2352 AAGCTT MamI 1178 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2040 GATAGTTATC NcoI 463 CCATGG C^CATGG 981 CCATGG NspV 1206 TTCGAA TT^CGAA 2020 TTCGAA PflFI 3022 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3347 GACAAGGTC PpuMI 1336 AGGACCC [GA]G^G[AT]CC[CT] 2429 AGGACCT SfuI 1206 TTCGAA TT^CGAA 2020 TTCGAA Tth111I 3022 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3347 GACAAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII