##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BfrI BglI BlnI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI BstEII BstPI ClaI CpoI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1010 GACGTC GACGT^C
AccII 870 CGCG CG^CG
AcyI 1010 GACGTC G[GA]^CG[CT]C
Alw44I 1823 GTGCAC G^TGCAC
Aor51HI 277 AGCGCT AGC^GCT
ApaI 2563 GGGCCC GGGCC^C
ApaLI 1823 GTGCAC G^TGCAC
Asp700I 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 39 CCCGGG C^[CT]CG[GA]G
BbvCI 818 CCTCAGC CCTCAGC(-5/-2)
BcgI 406 CGAAAGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1183 TGATCA T^GATCA
BglII 784 AGATCT A^GATCT
BsaAI 950 TACGTG [CT]AC^GT[GA]
BsaHI 1010 GACGTC G[GA]^CG[CT]C
BsiEI 1 CGGCCG CG[GA][CT]^CG
BsmBI 1008 GAGACG CGTCTC(1/5)
BsoBI 39 CCCGGG C^[CT]CG[GA]G
BspHI 1616 TCATGA T^CATGA
BsrBI 1515 GAGCGG CCGCTC(-3/-3)
Bst1107I 1423 GTATAC GTA^TAC
BstAPI 2742 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 870 CGCG CG^CG
BstXI 2438 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1423 GTATAC GTA^TAC
DraI 1367 TTTAAA TTT^AAA
DraIII 2618 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1 CGGCCG C^GGCCG
EclXI 1 CGGCCG C^GGCCG
Eco47III 277 AGCGCT AGC^GCT
Eco52I 1 CGGCCG C^GGCCG
FbaI 1183 TGATCA T^GATCA
HgaI 2365 GCGTC GACGC(5/10)
Hin1I 1010 GACGTC G[GA]^CG[CT]C
MvnI 870 CGCG CG^CG
NaeI 497 GCCGGC GCC^GGC
NdeI 2086 CATATG CA^TATG
NgoMIV 497 GCCGGC G^CCGGC
PshAI 3170 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1616 TCATGA T^CATGA
SapI 792 GCTCTTC GCTCTTC(1/4)
SbfI 2979 CCTGCAGG CCTGCA^GG
SmaI 39 CCCGGG CCC^GGG
Sse8387I 2979 CCTGCAGG CCTGCA^GG
XmaCI 39 CCCGGG C^CCGGG
XmaI 39 CCCGGG C^CCGGG
XmnI 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 1423 GTATAC GT^[AC][GT]AC
1683 GTAGAC
AcsI 518 AAATTT [GA]^AATT[CT]
2168 AAATTC
ApoI 518 AAATTT [GA]^AATT[CT]
2168 AAATTC
AspI 3022 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3347 GACAAGGTC
BsaBI 1178 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2040 GATAGTTATC
BstBI 1206 TTCGAA TT^CGAA
2020 TTCGAA
HaeII 277 AGCGCT [GA]GCGC^[CT]
822 AGCGCC
HindIII 2059 AAGCTT A^AGCTT
2352 AAGCTT
MamI 1178 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2040 GATAGTTATC
NcoI 463 CCATGG C^CATGG
981 CCATGG
NspV 1206 TTCGAA TT^CGAA
2020 TTCGAA
PflFI 3022 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3347 GACAAGGTC
PpuMI 1336 AGGACCC [GA]G^G[AT]CC[CT]
2429 AGGACCT
SfuI 1206 TTCGAA TT^CGAA
2020 TTCGAA
Tth111I 3022 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3347 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII