##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AccI AccIII AflII AgeI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI AvrII BamHI BbeI BbsI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BpuAI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI KspI MluI
MroI NarI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2222 GACGTC GACGT^C
Acc65I 1974 GGTACC G^GTACC
AclI 409 AACGTT AA^CGTT
AflIII 2721 ACGTGT A^C[GA][CT]GT
AhdI 1807 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2257 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1974 GGTACC G^GTACC
AspEI 1807 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1580 GAGCTC G[AT]GC[AT]^C
AspI 20 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2091 TGCGCA TGC^GCA
BcgI 2663 CGAGGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 624 TGATCA T^GATCA
BcnI 387 CCCGG CC^[GC]GG
BglI 2677 GCCCTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 960 AGATCT A^GATCT
BseRI 662 GAGGAG GAGGAG(10/8)
BsiHKAI 1580 GAGCTC G[AT]GC[AT]^C
BsmI 2257 GAATGC GAATGC(1/-1)
BspHI 27 TCATGA T^CATGA
BspMI 1564 GCAGGT ACCTGC(4/8)
BsrBI 1697 GAGCGG CCGCTC(-3/-3)
BsrDI 378 GCAATG GCAATG(2/0)
BsrFI 1793 GCCGGC [GA]^CCGG[CT]
BssSI 2155 CACGAG CACGAG(-5/-1)
BstAPI 1016 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1793 GCCGGC [GA]^CCGG[CT]
Eam1105I 1807 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 624 TGATCA T^GATCA
FspI 2091 TGCGCA TGC^GCA
HincII 1253 GTCAAC GT[CT]^[GA]AC
HindII 1253 GTCAAC GT[CT]^[GA]AC
KpnI 1974 GGTACC GGTAC^C
MfeI 810 CAATTG C^AATTG
MunI 810 CAATTG C^AATTG
NaeI 1793 GCCGGC GCC^GGC
NciI 387 CCCGG CC^[GC]GG
NcoI 1464 CCATGG C^CATGG
NdeI 708 CATATG CA^TATG
NgoMIV 1793 GCCGGC G^CCGGC
NspI 840 ACATGC [GA]CATG^[CT]
PflFI 20 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2362 GAGTC GAGTC(4/5)
PpuMI 1452 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 409 AACGTT AA^CGTT
RcaI 27 TCATGA T^CATGA
SacI 1580 GAGCTC GAGCT^C
ScaI 462 AGTACT AGT^ACT
SspI 179 AATATT AAT^ATT
Tth111I 20 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2257 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 719 GAATTT [GA]^AATT[CT]
2780 AAATTT
Aor51HI 2003 AGCGCT AGC^GCT
2053 AGCGCT
ApoI 719 GAATTT [GA]^AATT[CT]
2780 AAATTT
BanI 1662 GGCACC G^G[CT][GA]CC
1974 GGTACC
BbrPI 1829 CACGTG CAC^GTG
2720 CACGTG
BfaI 2509 CTAG C^TAG
2642 CTAG
BsaBI 979 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1841 GATGACAATC
Bsp1407I 449 TGTACA T^GTACA
917 TGTACA
BsrGI 449 TGTACA T^GTACA
917 TGTACA
BstYI 960 AGATCT [GA]^GATC[CT]
2240 AGATCC
DraII 12 AGGCCCT [GA]G^G[ACGT]CC[CT]
1452 AGGACCT
DsaI 1464 CCATGG C^C[GA][CT]GG
1737 CCGTGG
Eco47III 2003 AGCGCT AGC^GCT
2053 AGCGCT
EcoO109I 12 AGGCCCT [GA]G^G[ACGT]CC[CT]
1452 AGGACCT
HapII 388 CCGG C^CGG
1794 CCGG
HpaII 388 CCGG C^CGG
1794 CCGG
MaeI 2509 CTAG C^TAG
2642 CTAG
MamI 979 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1841 GATGACAATC
MflI 960 AGATCT [GA]^GATC[CT]
2240 AGATCC
MspI 388 CCGG C^CGG
1794 CCGG
PmaCI 1829 CACGTG CAC^GTG
2720 CACGTG
PmlI 1829 CACGTG CAC^GTG
2720 CACGTG
SexAI 1953 ACCTGGT A^CC[AT]GGT
1980 ACCTGGT
SspBI 449 TGTACA T^GTACA
917 TGTACA
XcmI 1330 CCACCCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1971 CCAGGTACCACCTGG
XhoII 960 AGATCT [GA]^GATC[CT]
2240 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BalI
BanII BbvI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraIII EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV PaeR7I PflMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoI