################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI ApaI AscI AseI AsnI AspEI AvaI AviII BamHI BbeI BbrPI BciVI BfrI BglI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I EcoNI EcoO65I EcoRV FseI FspI HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 412 ACGTGT A^C[GA][CT]GT Alw44I 881 GTGCAC G^TGCAC Aor51HI 869 AGCGCT AGC^GCT ApaLI 881 GTGCAC G^TGCAC Asp700I 631 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1636 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 154 CCTAGG C^CTAGG BbvCI 704 CCTCAGC CCTCAGC(-5/-2) BglII 433 AGATCT A^GATCT BlnI 154 CCTAGG C^CTAGG BsaAI 411 TACGTG [CT]AC^GT[GA] BsmBI 1146 GAGACG CGTCTC(1/5) BsmI 2981 GCATTC GAATGC(1/-1) BspHI 1938 TCATGA T^CATGA BsrFI 721 GCCGGT [GA]^CCGG[CT] BstXI 2901 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 433 AGATCT [GA]^GATC[CT] Cfr10I 721 GCCGGT [GA]^CCGG[CT] EagI 719 CGGCCG C^GGCCG EclXI 719 CGGCCG C^GGCCG Eco47III 869 AGCGCT AGC^GCT Eco52I 719 CGGCCG C^GGCCG EcoRI 1965 GAATTC G^AATTC EcoT22I 2209 ATGCAT ATGCA^T FauI 382 GCGGG CCCGC(4/6) HincII 973 GTCAAC GT[CT]^[GA]AC HindII 973 GTCAAC GT[CT]^[GA]AC MflI 433 AGATCT [GA]^GATC[CT] NcoI 2539 CCATGG C^CATGG NsiI 2209 ATGCAT ATGCA^T PflFI 1636 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2357 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 835 CGATCG CGAT^CG RcaI 1938 TCATGA T^CATGA SacI 1905 GAGCTC GAGCT^C SapI 2639 GAAGAGC GCTCTTC(1/4) ScaI 1473 AGTACT AGT^ACT SexAI 48 ACCTGGT A^CC[AT]GGT SspI 2072 AATATT AAT^ATT Tth111I 1636 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2357 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1840 CCAATGTGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 433 AGATCT [GA]^GATC[CT] XmnI 631 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 1384 AAATTT [GA]^AATT[CT] 1965 GAATTC AcyI 1148 GACGCC G[GA]^CG[CT]C 1479 GGCGTC AlwI 2160 GATCC GGATC(4/5) 2959 GGATC AlwNI 358 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2379 CAGCAACTG AocI 1226 CCTCAGG CC^T[ACGT]AGG 2457 CCTCAGG ApoI 1384 AAATTT [GA]^AATT[CT] 1965 GAATTC BcnI 571 CCCGG CC^[GC]GG 1497 CCCGG BpmI 2439 CTGGAG CTGGAG(16/14) 2760 CTGGAG BsaHI 1148 GACGCC G[GA]^CG[CT]C 1479 GGCGTC BsiEI 719 CGGCCG CG[GA][CT]^CG 835 CGATCG BspMI 1243 ACCTGC ACCTGC(4/8) 2711 ACCTGC BsrDI 94 CATTGC GCAATG(2/0) 2415 CATTGC Bsu36I 1226 CCTCAGG CC^T[ACGT]AGG 2457 CCTCAGG DrdI 964 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2139 GACAGGAGAGTC Eco81I 1226 CCTCAGG CC^T[ACGT]AGG 2457 CCTCAGG Hin1I 1148 GACGCC G[GA]^CG[CT]C 1479 GGCGTC HindIII 1399 AAGCTT A^AGCTT 2950 AAGCTT NciI 571 CCCGG CC^[GC]GG 1497 CCCGG NspI 1501 GCATGT [GA]CATG^[CT] 2006 ACATGC PstI 254 CTGCAG CTGCA^G 1607 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### Acc65I AciI AfaI AluI Asp718I AspHI AvaII BalI BanI BanII BbsI BbvI BcgI BclI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I