##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI ApaI AscI
AseI AsnI AspEI AvaI AviII
BamHI BbeI BbrPI BciVI BfrI
BglI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I EcoNI EcoO65I EcoRV FseI
FspI HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 412 ACGTGT A^C[GA][CT]GT
Alw44I 881 GTGCAC G^TGCAC
Aor51HI 869 AGCGCT AGC^GCT
ApaLI 881 GTGCAC G^TGCAC
Asp700I 631 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1636 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 154 CCTAGG C^CTAGG
BbvCI 704 CCTCAGC CCTCAGC(-5/-2)
BglII 433 AGATCT A^GATCT
BlnI 154 CCTAGG C^CTAGG
BsaAI 411 TACGTG [CT]AC^GT[GA]
BsmBI 1146 GAGACG CGTCTC(1/5)
BsmI 2981 GCATTC GAATGC(1/-1)
BspHI 1938 TCATGA T^CATGA
BsrFI 721 GCCGGT [GA]^CCGG[CT]
BstXI 2901 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 433 AGATCT [GA]^GATC[CT]
Cfr10I 721 GCCGGT [GA]^CCGG[CT]
EagI 719 CGGCCG C^GGCCG
EclXI 719 CGGCCG C^GGCCG
Eco47III 869 AGCGCT AGC^GCT
Eco52I 719 CGGCCG C^GGCCG
EcoRI 1965 GAATTC G^AATTC
EcoT22I 2209 ATGCAT ATGCA^T
FauI 382 GCGGG CCCGC(4/6)
HincII 973 GTCAAC GT[CT]^[GA]AC
HindII 973 GTCAAC GT[CT]^[GA]AC
MflI 433 AGATCT [GA]^GATC[CT]
NcoI 2539 CCATGG C^CATGG
NsiI 2209 ATGCAT ATGCA^T
PflFI 1636 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2357 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 835 CGATCG CGAT^CG
RcaI 1938 TCATGA T^CATGA
SacI 1905 GAGCTC GAGCT^C
SapI 2639 GAAGAGC GCTCTTC(1/4)
ScaI 1473 AGTACT AGT^ACT
SexAI 48 ACCTGGT A^CC[AT]GGT
SspI 2072 AATATT AAT^ATT
Tth111I 1636 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2357 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1840 CCAATGTGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 433 AGATCT [GA]^GATC[CT]
XmnI 631 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1384 AAATTT [GA]^AATT[CT]
1965 GAATTC
AcyI 1148 GACGCC G[GA]^CG[CT]C
1479 GGCGTC
AlwI 2160 GATCC GGATC(4/5)
2959 GGATC
AlwNI 358 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2379 CAGCAACTG
AocI 1226 CCTCAGG CC^T[ACGT]AGG
2457 CCTCAGG
ApoI 1384 AAATTT [GA]^AATT[CT]
1965 GAATTC
BcnI 571 CCCGG CC^[GC]GG
1497 CCCGG
BpmI 2439 CTGGAG CTGGAG(16/14)
2760 CTGGAG
BsaHI 1148 GACGCC G[GA]^CG[CT]C
1479 GGCGTC
BsiEI 719 CGGCCG CG[GA][CT]^CG
835 CGATCG
BspMI 1243 ACCTGC ACCTGC(4/8)
2711 ACCTGC
BsrDI 94 CATTGC GCAATG(2/0)
2415 CATTGC
Bsu36I 1226 CCTCAGG CC^T[ACGT]AGG
2457 CCTCAGG
DrdI 964 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2139 GACAGGAGAGTC
Eco81I 1226 CCTCAGG CC^T[ACGT]AGG
2457 CCTCAGG
Hin1I 1148 GACGCC G[GA]^CG[CT]C
1479 GGCGTC
HindIII 1399 AAGCTT A^AGCTT
2950 AAGCTT
NciI 571 CCCGG CC^[GC]GG
1497 CCCGG
NspI 1501 GCATGT [GA]CATG^[CT]
2006 ACATGC
PstI 254 CTGCAG CTGCA^G
1607 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
Acc65I AciI AfaI AluI Asp718I
AspHI AvaII BalI BanI BanII
BbsI BbvI BcgI BclI BfaI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I