################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AhdI AocI AscI AspEI AviII BalI BamHI BbeI BciVI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FseI FspI Hin1I HincII HindII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PaeR7I PflMI PinAI PmeI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1376 GGTACC G^GTACC AccI 1545 GTCTAC GT^[AC][GT]AC AccII 2200 CGCG CG^CG AlwI 720 GGATC GGATC(4/5) Aor51HI 947 AGCGCT AGC^GCT ApaI 521 GGGCCC GGGCC^C AseI 169 ATTAAT AT^TAAT AsnI 169 ATTAAT AT^TAAT Asp718I 1376 GGTACC G^GTACC AspI 1102 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 488 CCTAGG C^CTAGG BcgI 100 CGATGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2642 TGATCA T^GATCA BcnI 1792 CCCGG CC^[GC]GG BglII 1473 AGATCT A^GATCT BlnI 488 CCTAGG C^CTAGG BsmBI 912 CGTCTC CGTCTC(1/5) BsmI 1682 GAATGC GAATGC(1/-1) Bsp1407I 2605 TGTACA T^GTACA BsrGI 2605 TGTACA T^GTACA BssSI 1361 CTCGTG CACGAG(-5/-1) BstUI 2200 CGCG CG^CG BstXI 1214 CCAGTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1473 AGATCT [GA]^GATC[CT] EarI 2463 GAAGAG CTCTTC(1/4) Eco47III 947 AGCGCT AGC^GCT EcoRI 262 GAATTC G^AATTC EcoT22I 2625 ATGCAT ATGCA^T FbaI 2642 TGATCA T^GATCA HaeII 947 AGCGCT [GA]GCGC^[CT] HapII 1793 CCGG C^CGG HindIII 2553 AAGCTT A^AGCTT HpaII 1793 CCGG C^CGG KpnI 1376 GGTACC GGTAC^C Ksp632I 2463 GAAGAG CTCTTC(1/4) MflI 1473 AGATCT [GA]^GATC[CT] MspI 1793 CCGG C^CGG MvnI 2200 CGCG CG^CG NciI 1792 CCCGG CC^[GC]GG NdeI 2638 CATATG CA^TATG NsiI 2625 ATGCAT ATGCA^T PacI 1708 TTAATTAA TTAAT^TAA PflFI 1102 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2203 GACACTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 169 ATTAAT AT^TAAT SpeI 2068 ACTAGT A^CTAGT SspBI 2605 TGTACA T^GTACA Tth111I 1102 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 706 CCACTGGTCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1473 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 262 GAATTC [GA]^AATT[CT] 2949 AAATTC AflII 1117 CTTAAG C^TTAAG 2390 CTTAAG Alw44I 782 GTGCAC G^TGCAC 1972 GTGCAC ApaLI 782 GTGCAC G^TGCAC 1972 GTGCAC ApoI 262 GAATTC [GA]^AATT[CT] 2949 AAATTC Asp700I 2851 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2977 GAAAGAGTTC AvaI 794 CTCGGG C^[CT]CG[GA]G 2759 CCCGAG BbsI 402 GAAGAC GAAGAC(2/6) 3113 GTCTTC BbvCI 1296 CCTCAGC CCTCAGC(-5/-2) 1181 GCTGAGG BfrI 1117 CTTAAG C^TTAAG 2390 CTTAAG BpuAI 402 GAAGAC GAAGAC(2/6) 3113 GTCTTC BsoBI 794 CTCGGG C^[CT]CG[GA]G 2759 CCCGAG BspMI 428 ACCTGC ACCTGC(4/8) 530 ACCTGC BsrDI 674 CATTGC GCAATG(2/0) 2338 CATTGC CfoI 948 GCGC GCG^C 2199 GCGC DraI 2260 TTTAAA TTT^AAA 2915 TTTAAA HgaI 911 GCGTC GACGC(5/10) 2511 GCGTC HhaI 948 GCGC GCG^C 2199 GCGC HinP1I 948 GCGC G^CGC 2199 GCGC SmlI 1117 CTTAAG C^T[CT][GA]AG 2390 CTTAAG SphI 2623 GCATGC GCATG^C 2900 GCATGC XmnI 2851 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2977 GAAAGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AflIII AluI AlwNI AspHI AvaII BanI BanII BbrPI BbvI BfaI BglI BmyI BpmI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I