##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI AocI AscI AspEI AviII
BalI BamHI BbeI BciVI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV FseI FspI Hin1I HincII
HindII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PaeR7I
PflMI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1376 GGTACC G^GTACC
AccI 1545 GTCTAC GT^[AC][GT]AC
AccII 2200 CGCG CG^CG
AlwI 720 GGATC GGATC(4/5)
Aor51HI 947 AGCGCT AGC^GCT
ApaI 521 GGGCCC GGGCC^C
AseI 169 ATTAAT AT^TAAT
AsnI 169 ATTAAT AT^TAAT
Asp718I 1376 GGTACC G^GTACC
AspI 1102 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 488 CCTAGG C^CTAGG
BcgI 100 CGATGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2642 TGATCA T^GATCA
BcnI 1792 CCCGG CC^[GC]GG
BglII 1473 AGATCT A^GATCT
BlnI 488 CCTAGG C^CTAGG
BsmBI 912 CGTCTC CGTCTC(1/5)
BsmI 1682 GAATGC GAATGC(1/-1)
Bsp1407I 2605 TGTACA T^GTACA
BsrGI 2605 TGTACA T^GTACA
BssSI 1361 CTCGTG CACGAG(-5/-1)
BstUI 2200 CGCG CG^CG
BstXI 1214 CCAGTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1473 AGATCT [GA]^GATC[CT]
EarI 2463 GAAGAG CTCTTC(1/4)
Eco47III 947 AGCGCT AGC^GCT
EcoRI 262 GAATTC G^AATTC
EcoT22I 2625 ATGCAT ATGCA^T
FbaI 2642 TGATCA T^GATCA
HaeII 947 AGCGCT [GA]GCGC^[CT]
HapII 1793 CCGG C^CGG
HindIII 2553 AAGCTT A^AGCTT
HpaII 1793 CCGG C^CGG
KpnI 1376 GGTACC GGTAC^C
Ksp632I 2463 GAAGAG CTCTTC(1/4)
MflI 1473 AGATCT [GA]^GATC[CT]
MspI 1793 CCGG C^CGG
MvnI 2200 CGCG CG^CG
NciI 1792 CCCGG CC^[GC]GG
NdeI 2638 CATATG CA^TATG
NsiI 2625 ATGCAT ATGCA^T
PacI 1708 TTAATTAA TTAAT^TAA
PflFI 1102 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2203 GACACTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 169 ATTAAT AT^TAAT
SpeI 2068 ACTAGT A^CTAGT
SspBI 2605 TGTACA T^GTACA
Tth111I 1102 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 706 CCACTGGTCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1473 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 262 GAATTC [GA]^AATT[CT]
2949 AAATTC
AflII 1117 CTTAAG C^TTAAG
2390 CTTAAG
Alw44I 782 GTGCAC G^TGCAC
1972 GTGCAC
ApaLI 782 GTGCAC G^TGCAC
1972 GTGCAC
ApoI 262 GAATTC [GA]^AATT[CT]
2949 AAATTC
Asp700I 2851 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2977 GAAAGAGTTC
AvaI 794 CTCGGG C^[CT]CG[GA]G
2759 CCCGAG
BbsI 402 GAAGAC GAAGAC(2/6)
3113 GTCTTC
BbvCI 1296 CCTCAGC CCTCAGC(-5/-2)
1181 GCTGAGG
BfrI 1117 CTTAAG C^TTAAG
2390 CTTAAG
BpuAI 402 GAAGAC GAAGAC(2/6)
3113 GTCTTC
BsoBI 794 CTCGGG C^[CT]CG[GA]G
2759 CCCGAG
BspMI 428 ACCTGC ACCTGC(4/8)
530 ACCTGC
BsrDI 674 CATTGC GCAATG(2/0)
2338 CATTGC
CfoI 948 GCGC GCG^C
2199 GCGC
DraI 2260 TTTAAA TTT^AAA
2915 TTTAAA
HgaI 911 GCGTC GACGC(5/10)
2511 GCGTC
HhaI 948 GCGC GCG^C
2199 GCGC
HinP1I 948 GCGC G^CGC
2199 GCGC
SmlI 1117 CTTAAG C^T[CT][GA]AG
2390 CTTAAG
SphI 2623 GCATGC GCATG^C
2900 GCATGC
XmnI 2851 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2977 GAAAGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwNI
AspHI AvaII BanI BanII BbrPI
BbvI BfaI BglI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I