##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccII AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI BalI BbeI BbrPI
BbvCI BcgI BglI BsiEI BsiWI
BsmBI BspDI BsrBI BssHII BstAPI
BstBI BstEII BstPI BstUI BstXI
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI HpaI KasI
KspI MluI MluNI MscI MvnI
NarI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AatII 1764 GACGTC GACGT^C
Acc65I 2195 GGTACC G^GTACC
AccI 2632 GTATAC GT^[AC][GT]AC
AccIII 128 TCCGGA T^CCGGA
AcyI 1764 GACGTC G[GA]^CG[CT]C
AflIII 3235 ACATGT A^C[GA][CT]GT
AocI 1770 CCTGAGG CC^T[ACGT]AGG
AseI 240 ATTAAT AT^TAAT
AsnI 240 ATTAAT AT^TAAT
Asp718I 2195 GGTACC G^GTACC
AvaII 170 GGTCC G^G[AT]CC
AviII 2407 TGCGCA TGC^GCA
AvrII 2897 CCTAGG C^CTAGG
BamHI 272 GGATCC G^GATCC
BbsI 463 GTCTTC GAAGAC(2/6)
BclI 3467 TGATCA T^GATCA
BglII 1010 AGATCT A^GATCT
BlnI 2897 CCTAGG C^CTAGG
BpuAI 463 GTCTTC GAAGAC(2/6)
BsaAI 1791 TACGTG [CT]AC^GT[GA]
BsaBI 1952 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1764 GACGTC G[GA]^CG[CT]C
BsaI 1839 GAGACC GGTCTC(1/5)
BsaWI 128 TCCGGA [AT]^CCGG[AT]
BseAI 128 TCCGGA T^CCGGA
BsmI 1886 GAATGC GAATGC(1/-1)
Bsp1407I 3336 TGTACA T^GTACA
BspEI 128 TCCGGA T^CCGGA
BspLU11I 3235 ACATGT A^CATGT
BspMI 1339 GCAGGT ACCTGC(4/8)
BsrGI 3336 TGTACA T^GTACA
BssSI 1116 CACGAG CACGAG(-5/-1)
Bst1107I 2632 GTATAC GTA^TAC
BstZ17I 2632 GTATAC GTA^TAC
Bsu36I 1770 CCTGAGG CC^T[ACGT]AGG
Cfr13I 170 GGTCC G^G[ACGT]CC
DraII 169 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 1758 GACAGCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1770 CCTGAGG CC^T[ACGT]AGG
EcoO109I 169 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 2530 ATGCAT ATGCA^T
FauI 1784 GCGGG CCCGC(4/6)
FbaI 3467 TGATCA T^GATCA
FspI 2407 TGCGCA TGC^GCA
HaeII 1734 AGCGCC [GA]GCGC^[CT]
HgaI 569 GCGTC GACGC(5/10)
Hin1I 1764 GACGTC G[GA]^CG[CT]C
HincII 2873 GTTGAC GT[CT]^[GA]AC
HindII 2873 GTTGAC GT[CT]^[GA]AC
KpnI 2195 GGTACC GGTAC^C
MamI 1952 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2712 CAATTG C^AATTG
MroI 128 TCCGGA T^CCGGA
MunI 2712 CAATTG C^AATTG
NaeI 11 GCCGGC GCC^GGC
NcoI 1105 CCATGG C^CATGG
NdeI 900 CATATG CA^TATG
NgoMIV 11 GCCGGC G^CCGGC
NheI 537 GCTAGC G^CTAGC
NsiI 2530 ATGCAT ATGCA^T
PpuMI 169 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 240 ATTAAT AT^TAAT
SacI 2982 GAGCTC GAGCT^C
Sau96I 170 GGTCC G^G[ACGT]CC
SexAI 2881 ACCTGGT A^CC[AT]GGT
SspBI 3336 TGTACA T^GTACA
SspI 510 AATATT AAT^ATT
XcmI 3443 CCAGTGTTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 638 CTTAAG C^TTAAG
1962 CTTAAG
AlwNI 111 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1147 CAGCTGCTG
AvaI 1834 CCCGAG C^[CT]CG[GA]G
2992 CTCGGG
BanI 150 GGTGCC G^G[CT][GA]CC
2195 GGTACC
BcnI 106 CCCGG CC^[GC]GG
1234 CCCGG
BfrI 638 CTTAAG C^TTAAG
1962 CTTAAG
BsgI 485 CTGCAC GTGCAG(16/14)
967 GTGCAG
BsoBI 1834 CCCGAG C^[CT]CG[GA]G
2992 CTCGGG
BspHI 100 TCATGA T^CATGA
1529 TCATGA
CfoI 1735 GCGC GCG^C
2408 GCGC
DsaI 1105 CCATGG C^C[GA][CT]GG
1196 CCGTGG
HhaI 1735 GCGC GCG^C
2408 GCGC
HinP1I 1735 GCGC G^CGC
2408 GCGC
HphI 2885 GGTGA GGTGA(8/7)
1572 TCACC
NciI 106 CCCGG CC^[GC]GG
1234 CCCGG
PflMI 1105 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2416 CCATTTACTGG
RcaI 100 TCATGA T^CATGA
1529 TCATGA
ScaI 1173 AGTACT AGT^ACT
1627 AGTACT
StuI 545 AGGCCT AGG^CCT
2961 AGGCCT
Van91I 1105 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2416 CCATTTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BciVI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr10I DdeI DpnII
DraI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SapI Sau3AI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII