##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AspI BbeI BcgI
BciVI BfrI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 611 GACGTC GACGT^C
AcsI 2270 AAATTT [GA]^AATT[CT]
AflIII 3486 ACGTGT A^C[GA][CT]GT
AhdI 1142 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 204 AGCGCT AGC^GCT
ApoI 2270 AAATTT [GA]^AATT[CT]
AspEI 1142 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3560 CCTAGG C^CTAGG
BamHI 2641 GGATCC G^GATCC
BbrPI 3485 CACGTG CAC^GTG
BclI 2391 TGATCA T^GATCA
BglI 1936 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3560 CCTAGG C^CTAGG
BsmBI 1596 GAGACG CGTCTC(1/5)
BspHI 2590 TCATGA T^CATGA
Eam1105I 1142 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 204 AGCGCT AGC^GCT
FbaI 2391 TGATCA T^GATCA
HincII 1258 GTCAAC GT[CT]^[GA]AC
HindII 1258 GTCAAC GT[CT]^[GA]AC
NruI 1036 TCGCGA TCG^CGA
PmaCI 3485 CACGTG CAC^GTG
PmlI 3485 CACGTG CAC^GTG
PshAI 1177 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2590 TCATGA T^CATGA
SacI 1724 GAGCTC GAGCT^C
SbfI 1449 CCTGCAGG CCTGCA^GG
Sse8387I 1449 CCTGCAGG CCTGCA^GG
StuI 546 AGGCCT AGG^CCT
TfiI 779 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 855 TCCGGA T^CCGGA
2697 TCCGGA
AcyI 611 GACGTC G[GA]^CG[CT]C
1583 GACGCC
ApaI 813 GGGCCC GGGCC^C
1916 GGGCCC
AviII 921 TGCGCA TGC^GCA
2892 TGCGCA
BbsI 878 GAAGAC GAAGAC(2/6)
700 GTCTTC
BbvCI 970 CCTCAGC CCTCAGC(-5/-2)
2330 GCTGAGG
BpuAI 878 GAAGAC GAAGAC(2/6)
700 GTCTTC
BsaAI 1488 CACGTA [CT]AC^GT[GA]
3485 CACGTG
BsaHI 611 GACGTC G[GA]^CG[CT]C
1583 GACGCC
BsaI 1883 GAGACC GGTCTC(1/5)
1149 GGTCTC
BseAI 855 TCCGGA T^CCGGA
2697 TCCGGA
BspEI 855 TCCGGA T^CCGGA
2697 TCCGGA
BsrFI 2935 GCCGGC [GA]^CCGG[CT]
2976 GCCGGC
Cfr10I 2935 GCCGGC [GA]^CCGG[CT]
2976 GCCGGC
DrdI 19 GACCACGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
881 GACAGTGTGGTC
EarI 3051 CTCTTC CTCTTC(1/4)
2815 GAAGAG
FspI 921 TGCGCA TGC^GCA
2892 TGCGCA
Hin1I 611 GACGTC G[GA]^CG[CT]C
1583 GACGCC
Ksp632I 3051 CTCTTC CTCTTC(1/4)
2815 GAAGAG
MroI 855 TCCGGA T^CCGGA
2697 TCCGGA
NaeI 2935 GCCGGC GCC^GGC
2976 GCCGGC
NgoMIV 2935 GCCGGC G^CCGGC
2976 GCCGGC
NspI 108 ACATGC [GA]CATG^[CT]
2556 ACATGC
PflMI 1904 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2362 CCAGATCCTGG
PvuII 953 CAGCTG CAG^CTG
2514 CAGCTG
ScaI 1269 AGTACT AGT^ACT
1815 AGTACT
Van91I 1904 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2362 CCAGATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglII BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI