################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII BalI BanI BbeI BglI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1332 GACGTC GACGT^C AflII 2635 CTTAAG C^TTAAG AseI 3302 ATTAAT AT^TAAT AsnI 3302 ATTAAT AT^TAAT Asp700I 741 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 907 CCTAGG C^CTAGG BamHI 2760 GGATCC G^GATCC BbrPI 726 CACGTG CAC^GTG BbvCI 2347 CCTCAGC CCTCAGC(-5/-2) BcgI 34 CGACTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1968 GGATAC GTATCC(6/5) BclI 3183 TGATCA T^GATCA BfrI 2635 CTTAAG C^TTAAG BglII 1598 AGATCT A^GATCT BlnI 907 CCTAGG C^CTAGG BsaAI 726 CACGTG [CT]AC^GT[GA] BsaWI 993 ACCGGA [AT]^CCGG[AT] BseRI 466 CTCCTC GAGGAG(10/8) BsiEI 6 CGGTCG CG[GA][CT]^CG Bsp1407I 27 TGTACA T^GTACA BsrDI 773 GCAATG GCAATG(2/0) BsrGI 27 TGTACA T^GTACA BstAPI 877 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 23 GCGC GCG^C DraIII 2456 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1326 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2400 TGGCCG [CT]^GGCC[GA] EcoNI 500 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 997 GATATC GAT^ATC FbaI 3183 TGATCA T^GATCA HhaI 23 GCGC GCG^C HinP1I 23 GCGC G^CGC HincII 2974 GTCAAC GT[CT]^[GA]AC HindII 2974 GTCAAC GT[CT]^[GA]AC PaeR7I 307 CTCGAG C^TCGAG PflMI 855 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 726 CACGTG CAC^GTG PmeI 1799 GTTTAAAC GTTT^AAAC PmlI 726 CACGTG CAC^GTG PshAI 966 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3302 ATTAAT AT^TAAT SapI 3024 GAAGAGC GCTCTTC(1/4) ScaI 1748 AGTACT AGT^ACT SexAI 146 ACCAGGT A^CC[AT]GGT SpeI 3197 ACTAGT A^CTAGT SspBI 27 TGTACA T^GTACA Van91I 855 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 307 CTCGAG C^TCGAG XmnI 741 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1213 GTAGAC GT^[AC][GT]AC 2803 GTAGAC AocI 1821 CCTGAGG CC^T[ACGT]AGG 2359 CCTCAGG BlpI 189 GCTCAGC GC^T[ACGT]AGC 3153 GCTGAGC Bpu1102I 189 GCTCAGC GC^T[ACGT]AGC 3153 GCTGAGC BsaI 560 GAGACC GGTCTC(1/5) 2203 GAGACC BsmI 396 GAATGC GAATGC(1/-1) 2337 GAATGC BsrBI 2108 CCGCTC CCGCTC(-3/-3) 2011 GAGCGG BstXI 1766 CCAACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2784 CCAGTCTTTTGG Bsu36I 1821 CCTGAGG CC^T[ACGT]AGG 2359 CCTCAGG CelII 189 GCTCAGC GC^T[ACGT]AGC 3153 GCTGAGC DraI 1800 TTTAAA TTT^AAA 3374 TTTAAA DsaI 129 CCACGG C^C[GA][CT]GG 686 CCGTGG EarI 75 GAAGAG CTCTTC(1/4) 3024 GAAGAG Eco81I 1821 CCTGAGG CC^T[ACGT]AGG 2359 CCTCAGG EcoRI 913 GAATTC G^AATTC 1569 GAATTC EcoT22I 2032 ATGCAT ATGCA^T 3309 ATGCAT FauI 2433 CCCGC CCCGC(4/6) 12 GCGGG HindIII 1079 AAGCTT A^AGCTT 1708 AAGCTT Ksp632I 75 GAAGAG CTCTTC(1/4) 3024 GAAGAG MaeII 727 ACGT A^CGT 1333 ACGT NsiI 2032 ATGCAT ATGCA^T 3309 ATGCAT PstI 652 CTGCAG CTGCA^G 1384 CTGCAG SspI 1463 AATATT AAT^ATT 2049 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII