##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
BalI BanI BbeI BglI BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI HaeII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1332 GACGTC GACGT^C
AflII 2635 CTTAAG C^TTAAG
AseI 3302 ATTAAT AT^TAAT
AsnI 3302 ATTAAT AT^TAAT
Asp700I 741 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 907 CCTAGG C^CTAGG
BamHI 2760 GGATCC G^GATCC
BbrPI 726 CACGTG CAC^GTG
BbvCI 2347 CCTCAGC CCTCAGC(-5/-2)
BcgI 34 CGACTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1968 GGATAC GTATCC(6/5)
BclI 3183 TGATCA T^GATCA
BfrI 2635 CTTAAG C^TTAAG
BglII 1598 AGATCT A^GATCT
BlnI 907 CCTAGG C^CTAGG
BsaAI 726 CACGTG [CT]AC^GT[GA]
BsaWI 993 ACCGGA [AT]^CCGG[AT]
BseRI 466 CTCCTC GAGGAG(10/8)
BsiEI 6 CGGTCG CG[GA][CT]^CG
Bsp1407I 27 TGTACA T^GTACA
BsrDI 773 GCAATG GCAATG(2/0)
BsrGI 27 TGTACA T^GTACA
BstAPI 877 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 23 GCGC GCG^C
DraIII 2456 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1326 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2400 TGGCCG [CT]^GGCC[GA]
EcoNI 500 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 997 GATATC GAT^ATC
FbaI 3183 TGATCA T^GATCA
HhaI 23 GCGC GCG^C
HinP1I 23 GCGC G^CGC
HincII 2974 GTCAAC GT[CT]^[GA]AC
HindII 2974 GTCAAC GT[CT]^[GA]AC
PaeR7I 307 CTCGAG C^TCGAG
PflMI 855 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 726 CACGTG CAC^GTG
PmeI 1799 GTTTAAAC GTTT^AAAC
PmlI 726 CACGTG CAC^GTG
PshAI 966 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3302 ATTAAT AT^TAAT
SapI 3024 GAAGAGC GCTCTTC(1/4)
ScaI 1748 AGTACT AGT^ACT
SexAI 146 ACCAGGT A^CC[AT]GGT
SpeI 3197 ACTAGT A^CTAGT
SspBI 27 TGTACA T^GTACA
Van91I 855 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 307 CTCGAG C^TCGAG
XmnI 741 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1213 GTAGAC GT^[AC][GT]AC
2803 GTAGAC
AocI 1821 CCTGAGG CC^T[ACGT]AGG
2359 CCTCAGG
BlpI 189 GCTCAGC GC^T[ACGT]AGC
3153 GCTGAGC
Bpu1102I 189 GCTCAGC GC^T[ACGT]AGC
3153 GCTGAGC
BsaI 560 GAGACC GGTCTC(1/5)
2203 GAGACC
BsmI 396 GAATGC GAATGC(1/-1)
2337 GAATGC
BsrBI 2108 CCGCTC CCGCTC(-3/-3)
2011 GAGCGG
BstXI 1766 CCAACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2784 CCAGTCTTTTGG
Bsu36I 1821 CCTGAGG CC^T[ACGT]AGG
2359 CCTCAGG
CelII 189 GCTCAGC GC^T[ACGT]AGC
3153 GCTGAGC
DraI 1800 TTTAAA TTT^AAA
3374 TTTAAA
DsaI 129 CCACGG C^C[GA][CT]GG
686 CCGTGG
EarI 75 GAAGAG CTCTTC(1/4)
3024 GAAGAG
Eco81I 1821 CCTGAGG CC^T[ACGT]AGG
2359 CCTCAGG
EcoRI 913 GAATTC G^AATTC
1569 GAATTC
EcoT22I 2032 ATGCAT ATGCA^T
3309 ATGCAT
FauI 2433 CCCGC CCCGC(4/6)
12 GCGGG
HindIII 1079 AAGCTT A^AGCTT
1708 AAGCTT
Ksp632I 75 GAAGAG CTCTTC(1/4)
3024 GAAGAG
MaeII 727 ACGT A^CGT
1333 ACGT
NsiI 2032 ATGCAT ATGCA^T
3309 ATGCAT
PstI 652 CTGCAG CTGCA^G
1384 CTGCAG
SspI 1463 AATATT AAT^ATT
2049 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII