##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AvrII BamHI BbrPI BbsI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DsaI
Eam1105I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MroI MseI
MunI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfuI
SgrAI SmaI SpeI Sse8387I SspBI
SwaI Tru9I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1376 GGTACC G^GTACC
AccI 2953 GTAGAC GT^[AC][GT]AC
AccII 1042 CGCG CG^CG
AcsI 826 GAATTC [GA]^AATT[CT]
AcyI 1507 GGCGCC G[GA]^CG[CT]C
Alw44I 1309 GTGCAC G^TGCAC
ApaI 1488 GGGCCC GGGCC^C
ApaLI 1309 GTGCAC G^TGCAC
ApoI 826 GAATTC [GA]^AATT[CT]
Asp718I 1376 GGTACC G^GTACC
AviII 2085 TGCGCA TGC^GCA
BbeI 1507 GGCGCC GGCGC^C
BfaI 1520 CTAG C^TAG
BglII 923 AGATCT A^GATCT
BsaHI 1507 GGCGCC G[GA]^CG[CT]C
BsaI 770 GAGACC GGTCTC(1/5)
BsaWI 797 TCCGGT [AT]^CCGG[AT]
BsmBI 756 CGTCTC CGTCTC(1/5)
BspLU11I 1256 ACATGT A^CATGT
BsrBI 417 GAGCGG CCGCTC(-3/-3)
BssHII 1041 GCGCGC G^CGCGC
BstEII 67 GGTGACC G^GT[ACGT]ACC
BstPI 67 GGTGACC G^GT[ACGT]ACC
BstUI 1042 CGCG CG^CG
EagI 248 CGGCCG C^GGCCG
EclXI 248 CGGCCG C^GGCCG
Eco52I 248 CGGCCG C^GGCCG
EcoO65I 67 GGTGACC G^GT[ACGT]ACC
EcoRI 826 GAATTC G^AATTC
FspI 2085 TGCGCA TGC^GCA
HgaI 519 GCGTC GACGC(5/10)
Hin1I 1507 GGCGCC G[GA]^CG[CT]C
KasI 1507 GGCGCC G^GCGCC
KpnI 1376 GGTACC GGTAC^C
MaeI 1520 CTAG C^TAG
MvnI 1042 CGCG CG^CG
NaeI 324 GCCGGC GCC^GGC
NarI 1507 GGCGCC GG^CGCC
NdeI 1762 CATATG CA^TATG
NgoMIV 324 GCCGGC G^CCGGC
PflMI 2631 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 131 ACCTGGT A^CC[AT]GGT
SfiI 2571 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1507 GGCGCC GGC^GCC
SnaBI 373 TACGTA TAC^GTA
SspI 1033 AATATT AAT^ATT
Van91I 2631 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 540 ACGTGT A^C[GA][CT]GT
1256 ACATGT
AspI 478 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1167 GACTCTGTC
AvaI 947 CCCGAG C^[CT]CG[GA]G
2026 CCCGAG
BbvCI 2318 CCTCAGC CCTCAGC(-5/-2)
394 GCTGAGG
BglI 2260 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2572 GCCAGCGAGGC
BsaAI 373 TACGTA [CT]AC^GT[GA]
1441 TACGTG
BsgI 565 CTGCAC GTGCAG(16/14)
2405 CTGCAC
BsmI 187 GAATGC GAATGC(1/-1)
2323 GCATTC
BsoBI 947 CCCGAG C^[CT]CG[GA]G
2026 CCCGAG
BstAPI 280 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
495 GCAGAAAGTGC
DrdI 1390 GACTCCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1863 GACCGGCGAGTC
EcoNI 1995 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2520 CCTGCCTCAGG
PflFI 478 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1167 GACTCTGTC
SphI 689 GCATGC GCATG^C
2088 GCATGC
TaqI 206 TCGA T^CGA
2645 TCGA
Tth111I 478 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1167 GACTCTGTC
TthHB8I 206 TCGA T^CGA
2645 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Aor51HI AspHI AvaII BalI
BanI BanII BbvI BcgI BcnI
BmyI BpmI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco47III Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI TseI Tsp45I Tsp509I TspRI
XhoII