################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbrPI BbsI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII DsaI Eam1105I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfuI SgrAI SmaI SpeI Sse8387I SspBI SwaI Tru9I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 1376 GGTACC G^GTACC AccI 2953 GTAGAC GT^[AC][GT]AC AccII 1042 CGCG CG^CG AcsI 826 GAATTC [GA]^AATT[CT] AcyI 1507 GGCGCC G[GA]^CG[CT]C Alw44I 1309 GTGCAC G^TGCAC ApaI 1488 GGGCCC GGGCC^C ApaLI 1309 GTGCAC G^TGCAC ApoI 826 GAATTC [GA]^AATT[CT] Asp718I 1376 GGTACC G^GTACC AviII 2085 TGCGCA TGC^GCA BbeI 1507 GGCGCC GGCGC^C BfaI 1520 CTAG C^TAG BglII 923 AGATCT A^GATCT BsaHI 1507 GGCGCC G[GA]^CG[CT]C BsaI 770 GAGACC GGTCTC(1/5) BsaWI 797 TCCGGT [AT]^CCGG[AT] BsmBI 756 CGTCTC CGTCTC(1/5) BspLU11I 1256 ACATGT A^CATGT BsrBI 417 GAGCGG CCGCTC(-3/-3) BssHII 1041 GCGCGC G^CGCGC BstEII 67 GGTGACC G^GT[ACGT]ACC BstPI 67 GGTGACC G^GT[ACGT]ACC BstUI 1042 CGCG CG^CG EagI 248 CGGCCG C^GGCCG EclXI 248 CGGCCG C^GGCCG Eco52I 248 CGGCCG C^GGCCG EcoO65I 67 GGTGACC G^GT[ACGT]ACC EcoRI 826 GAATTC G^AATTC FspI 2085 TGCGCA TGC^GCA HgaI 519 GCGTC GACGC(5/10) Hin1I 1507 GGCGCC G[GA]^CG[CT]C KasI 1507 GGCGCC G^GCGCC KpnI 1376 GGTACC GGTAC^C MaeI 1520 CTAG C^TAG MvnI 1042 CGCG CG^CG NaeI 324 GCCGGC GCC^GGC NarI 1507 GGCGCC GG^CGCC NdeI 1762 CATATG CA^TATG NgoMIV 324 GCCGGC G^CCGGC PflMI 2631 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SexAI 131 ACCTGGT A^CC[AT]GGT SfiI 2571 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1507 GGCGCC GGC^GCC SnaBI 373 TACGTA TAC^GTA SspI 1033 AATATT AAT^ATT Van91I 2631 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 540 ACGTGT A^C[GA][CT]GT 1256 ACATGT AspI 478 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1167 GACTCTGTC AvaI 947 CCCGAG C^[CT]CG[GA]G 2026 CCCGAG BbvCI 2318 CCTCAGC CCTCAGC(-5/-2) 394 GCTGAGG BglI 2260 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2572 GCCAGCGAGGC BsaAI 373 TACGTA [CT]AC^GT[GA] 1441 TACGTG BsgI 565 CTGCAC GTGCAG(16/14) 2405 CTGCAC BsmI 187 GAATGC GAATGC(1/-1) 2323 GCATTC BsoBI 947 CCCGAG C^[CT]CG[GA]G 2026 CCCGAG BstAPI 280 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 495 GCAGAAAGTGC DrdI 1390 GACTCCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1863 GACCGGCGAGTC EcoNI 1995 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2520 CCTGCCTCAGG PflFI 478 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1167 GACTCTGTC SphI 689 GCATGC GCATG^C 2088 GCATGC TaqI 206 TCGA T^CGA 2645 TCGA Tth111I 478 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1167 GACTCTGTC TthHB8I 206 TCGA T^CGA 2645 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI Aor51HI AspHI AvaII BalI BanI BanII BbvI BcgI BcnI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco47III Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI TseI Tsp45I Tsp509I TspRI XhoII