##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AviII BbrPI BcgI BclI
BfrI BglII BsaAI BsaBI BsiWI
BsmBI BspDI BssHII Bst1107I BstZ17I
ClaI CpoI DraIII DrdI Eco47III
EcoNI EcoRI FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MunI NheI NotI NruI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SgrAI
SmlI SnaBI SpeI Sse8387I SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 537 GTAGAC GT^[AC][GT]AC
AccIII 702 TCCGGA T^CCGGA
AflIII 3065 ACATGT A^C[GA][CT]GT
AhdI 2727 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1512 CCTCAGG CC^T[ACGT]AGG
AspEI 2727 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2878 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1866 CCTAGG C^CTAGG
BalI 238 TGGCCA TGG^CCA
BamHI 3159 GGATCC G^GATCC
BbsI 1684 GTCTTC GAAGAC(2/6)
BbvCI 1134 CCTCAGC CCTCAGC(-5/-2)
BglI 154 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1866 CCTAGG C^CTAGG
BpuAI 1684 GTCTTC GAAGAC(2/6)
BsaI 490 GAGACC GGTCTC(1/5)
BsaWI 702 TCCGGA [AT]^CCGG[AT]
BseAI 702 TCCGGA T^CCGGA
BsiEI 271 CGGCCG CG[GA][CT]^CG
Bsp1407I 3552 TGTACA T^GTACA
BspEI 702 TCCGGA T^CCGGA
BspHI 454 TCATGA T^CATGA
BspLU11I 3065 ACATGT A^CATGT
BsrBI 175 CCGCTC CCGCTC(-3/-3)
BsrFI 1501 GCCGGC [GA]^CCGG[CT]
BsrGI 3552 TGTACA T^GTACA
BstBI 2712 TTCGAA TT^CGAA
Bsu36I 1512 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1501 GCCGGC [GA]^CCGG[CT]
DraI 2764 TTTAAA TTT^AAA
EagI 271 CGGCCG C^GGCCG
Eam1105I 2727 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 271 CGGCCG C^GGCCG
Eco52I 271 CGGCCG C^GGCCG
Eco81I 1512 CCTCAGG CC^T[ACGT]AGG
EcoRV 2250 GATATC GAT^ATC
MluNI 238 TGGCCA TGG^CCA
MroI 702 TCCGGA T^CCGGA
MscI 238 TGGCCA TGG^CCA
NaeI 1501 GCCGGC GCC^GGC
NcoI 1627 CCATGG C^CATGG
NdeI 2894 CATATG CA^TATG
NgoMIV 1501 GCCGGC G^CCGGC
NspV 2712 TTCGAA TT^CGAA
PacI 2336 TTAATTAA TTAAT^TAA
PflFI 2878 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 454 TCATGA T^CATGA
SfuI 2712 TTCGAA TT^CGAA
SmaI 156 CCCGGG CCC^GGG
SphI 2657 GCATGC GCATG^C
SspBI 3552 TGTACA T^GTACA
StuI 935 AGGCCT AGG^CCT
Tth111I 2878 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2274 CCATTGACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 156 CCCGGG C^CCGGG
XmaI 156 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 38 CGCG CG^CG
3309 CGCG
AclI 2079 AACGTT AA^CGTT
2259 AACGTT
AlwNI 628 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1566 CAGTGACTG
AseI 1014 ATTAAT AT^TAAT
1709 ATTAAT
AsnI 1014 ATTAAT AT^TAAT
1709 ATTAAT
AvaI 156 CCCGGG C^[CT]CG[GA]G
442 CTCGGG
BbeI 61 GGCGCC GGCGC^C
162 GGCGCC
BsgI 2004 CTGCAC GTGCAG(16/14)
1641 GTGCAG
BsoBI 156 CCCGGG C^[CT]CG[GA]G
442 CTCGGG
BspMI 379 ACCTGC ACCTGC(4/8)
671 ACCTGC
BssSI 388 CACGAG CACGAG(-5/-1)
2567 CACGAG
BstEII 1113 GGTTACC G^GT[ACGT]ACC
2269 GGTGACC
BstPI 1113 GGTTACC G^GT[ACGT]ACC
2269 GGTGACC
BstUI 38 CGCG CG^CG
3309 CGCG
BstXI 574 CCAATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2524 CCACAGGGCTGG
BstYI 2707 GGATCT [GA]^GATC[CT]
3159 GGATCC
DsaI 208 CCGTGG C^C[GA][CT]GG
1627 CCATGG
EaeI 238 TGGCCA [CT]^GGCC[GA]
271 CGGCCG
EcoO65I 1113 GGTTACC G^GT[ACGT]ACC
2269 GGTGACC
EcoT22I 708 ATGCAT ATGCA^T
2655 ATGCAT
HindIII 279 AAGCTT A^AGCTT
2831 AAGCTT
KasI 61 GGCGCC G^GCGCC
162 GGCGCC
MflI 2707 GGATCT [GA]^GATC[CT]
3159 GGATCC
MslI 1846 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2503 CATAAAAATG
MvnI 38 CGCG CG^CG
3309 CGCG
NarI 61 GGCGCC GG^CGCC
162 GGCGCC
NsiI 708 ATGCAT ATGCA^T
2655 ATGCAT
PflMI 1092 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1565 CCAGTGACTGG
PshBI 1014 ATTAAT AT^TAAT
1709 ATTAAT
Psp1406I 2079 AACGTT AA^CGTT
2259 AACGTT
PvuII 1183 CAGCTG CAG^CTG
1644 CAGCTG
SacI 1981 GAGCTC GAGCT^C
3079 GAGCTC
SexAI 328 ACCTGGT A^CC[AT]GGT
1450 ACCAGGT
SfoI 61 GGCGCC GGC^GCC
162 GGCGCC
SspI 3455 AATATT AAT^ATT
3626 AATATT
TaqI 1496 TCGA T^CGA
2713 TCGA
TthHB8I 1496 TCGA T^CGA
2713 TCGA
Van91I 1092 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1565 CCAGTGACTGG
XhoII 2707 GGATCT [GA]^GATC[CT]
3159 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI