################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AviII BbrPI BcgI BclI BfrI BglII BsaAI BsaBI BsiWI BsmBI BspDI BssHII Bst1107I BstZ17I ClaI CpoI DraIII DrdI Eco47III EcoNI EcoRI FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MunI NheI NotI NruI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SgrAI SmlI SnaBI SpeI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 537 GTAGAC GT^[AC][GT]AC AccIII 702 TCCGGA T^CCGGA AflIII 3065 ACATGT A^C[GA][CT]GT AhdI 2727 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1512 CCTCAGG CC^T[ACGT]AGG AspEI 2727 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2878 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1866 CCTAGG C^CTAGG BalI 238 TGGCCA TGG^CCA BamHI 3159 GGATCC G^GATCC BbsI 1684 GTCTTC GAAGAC(2/6) BbvCI 1134 CCTCAGC CCTCAGC(-5/-2) BglI 154 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1866 CCTAGG C^CTAGG BpuAI 1684 GTCTTC GAAGAC(2/6) BsaI 490 GAGACC GGTCTC(1/5) BsaWI 702 TCCGGA [AT]^CCGG[AT] BseAI 702 TCCGGA T^CCGGA BsiEI 271 CGGCCG CG[GA][CT]^CG Bsp1407I 3552 TGTACA T^GTACA BspEI 702 TCCGGA T^CCGGA BspHI 454 TCATGA T^CATGA BspLU11I 3065 ACATGT A^CATGT BsrBI 175 CCGCTC CCGCTC(-3/-3) BsrFI 1501 GCCGGC [GA]^CCGG[CT] BsrGI 3552 TGTACA T^GTACA BstBI 2712 TTCGAA TT^CGAA Bsu36I 1512 CCTCAGG CC^T[ACGT]AGG Cfr10I 1501 GCCGGC [GA]^CCGG[CT] DraI 2764 TTTAAA TTT^AAA EagI 271 CGGCCG C^GGCCG Eam1105I 2727 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 271 CGGCCG C^GGCCG Eco52I 271 CGGCCG C^GGCCG Eco81I 1512 CCTCAGG CC^T[ACGT]AGG EcoRV 2250 GATATC GAT^ATC MluNI 238 TGGCCA TGG^CCA MroI 702 TCCGGA T^CCGGA MscI 238 TGGCCA TGG^CCA NaeI 1501 GCCGGC GCC^GGC NcoI 1627 CCATGG C^CATGG NdeI 2894 CATATG CA^TATG NgoMIV 1501 GCCGGC G^CCGGC NspV 2712 TTCGAA TT^CGAA PacI 2336 TTAATTAA TTAAT^TAA PflFI 2878 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 454 TCATGA T^CATGA SfuI 2712 TTCGAA TT^CGAA SmaI 156 CCCGGG CCC^GGG SphI 2657 GCATGC GCATG^C SspBI 3552 TGTACA T^GTACA StuI 935 AGGCCT AGG^CCT Tth111I 2878 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2274 CCATTGACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 156 CCCGGG C^CCGGG XmaI 156 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 38 CGCG CG^CG 3309 CGCG AclI 2079 AACGTT AA^CGTT 2259 AACGTT AlwNI 628 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 1566 CAGTGACTG AseI 1014 ATTAAT AT^TAAT 1709 ATTAAT AsnI 1014 ATTAAT AT^TAAT 1709 ATTAAT AvaI 156 CCCGGG C^[CT]CG[GA]G 442 CTCGGG BbeI 61 GGCGCC GGCGC^C 162 GGCGCC BsgI 2004 CTGCAC GTGCAG(16/14) 1641 GTGCAG BsoBI 156 CCCGGG C^[CT]CG[GA]G 442 CTCGGG BspMI 379 ACCTGC ACCTGC(4/8) 671 ACCTGC BssSI 388 CACGAG CACGAG(-5/-1) 2567 CACGAG BstEII 1113 GGTTACC G^GT[ACGT]ACC 2269 GGTGACC BstPI 1113 GGTTACC G^GT[ACGT]ACC 2269 GGTGACC BstUI 38 CGCG CG^CG 3309 CGCG BstXI 574 CCAATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2524 CCACAGGGCTGG BstYI 2707 GGATCT [GA]^GATC[CT] 3159 GGATCC DsaI 208 CCGTGG C^C[GA][CT]GG 1627 CCATGG EaeI 238 TGGCCA [CT]^GGCC[GA] 271 CGGCCG EcoO65I 1113 GGTTACC G^GT[ACGT]ACC 2269 GGTGACC EcoT22I 708 ATGCAT ATGCA^T 2655 ATGCAT HindIII 279 AAGCTT A^AGCTT 2831 AAGCTT KasI 61 GGCGCC G^GCGCC 162 GGCGCC MflI 2707 GGATCT [GA]^GATC[CT] 3159 GGATCC MslI 1846 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2503 CATAAAAATG MvnI 38 CGCG CG^CG 3309 CGCG NarI 61 GGCGCC GG^CGCC 162 GGCGCC NsiI 708 ATGCAT ATGCA^T 2655 ATGCAT PflMI 1092 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1565 CCAGTGACTGG PshBI 1014 ATTAAT AT^TAAT 1709 ATTAAT Psp1406I 2079 AACGTT AA^CGTT 2259 AACGTT PvuII 1183 CAGCTG CAG^CTG 1644 CAGCTG SacI 1981 GAGCTC GAGCT^C 3079 GAGCTC SexAI 328 ACCTGGT A^CC[AT]GGT 1450 ACCAGGT SfoI 61 GGCGCC GGC^GCC 162 GGCGCC SspI 3455 AATATT AAT^ATT 3626 AATATT TaqI 1496 TCGA T^CGA 2713 TCGA TthHB8I 1496 TCGA T^CGA 2713 TCGA Van91I 1092 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1565 CCAGTGACTGG XhoII 2707 GGATCT [GA]^GATC[CT] 3159 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI