##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AviII       BbrPI       BcgI        BclI        
BfrI        BglII       BsaAI       BsaBI       BsiWI       
BsmBI       BspDI       BssHII      Bst1107I    BstZ17I     
ClaI        CpoI        DraIII      DrdI        Eco47III    
EcoNI       EcoRI       FbaI        FseI        FspI        
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NheI        NotI        NruI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SwaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          537  GTAGAC               GT^[AC][GT]AC
AccIII        702  TCCGGA               T^CCGGA
AflIII       3065  ACATGT               A^C[GA][CT]GT
AhdI         2727  GACACAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1512  CCTCAGG              CC^T[ACGT]AGG
AspEI        2727  GACACAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2878  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1866  CCTAGG               C^CTAGG
BalI          238  TGGCCA               TGG^CCA
BamHI        3159  GGATCC               G^GATCC
BbsI         1684  GTCTTC               GAAGAC(2/6)
BbvCI        1134  CCTCAGC              CCTCAGC(-5/-2)
BglI          154  GCCCCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1866  CCTAGG               C^CTAGG
BpuAI        1684  GTCTTC               GAAGAC(2/6)
BsaI          490  GAGACC               GGTCTC(1/5)
BsaWI         702  TCCGGA               [AT]^CCGG[AT]
BseAI         702  TCCGGA               T^CCGGA
BsiEI         271  CGGCCG               CG[GA][CT]^CG
Bsp1407I     3552  TGTACA               T^GTACA
BspEI         702  TCCGGA               T^CCGGA
BspHI         454  TCATGA               T^CATGA
BspLU11I     3065  ACATGT               A^CATGT
BsrBI         175  CCGCTC               CCGCTC(-3/-3)
BsrFI        1501  GCCGGC               [GA]^CCGG[CT]
BsrGI        3552  TGTACA               T^GTACA
BstBI        2712  TTCGAA               TT^CGAA
Bsu36I       1512  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1501  GCCGGC               [GA]^CCGG[CT]
DraI         2764  TTTAAA               TTT^AAA
EagI          271  CGGCCG               C^GGCCG
Eam1105I     2727  GACACAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         271  CGGCCG               C^GGCCG
Eco52I        271  CGGCCG               C^GGCCG
Eco81I       1512  CCTCAGG              CC^T[ACGT]AGG
EcoRV        2250  GATATC               GAT^ATC
MluNI         238  TGGCCA               TGG^CCA
MroI          702  TCCGGA               T^CCGGA
MscI          238  TGGCCA               TGG^CCA
NaeI         1501  GCCGGC               GCC^GGC
NcoI         1627  CCATGG               C^CATGG
NdeI         2894  CATATG               CA^TATG
NgoMIV       1501  GCCGGC               G^CCGGC
NspV         2712  TTCGAA               TT^CGAA
PacI         2336  TTAATTAA             TTAAT^TAA
PflFI        2878  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          454  TCATGA               T^CATGA
SfuI         2712  TTCGAA               TT^CGAA
SmaI          156  CCCGGG               CCC^GGG
SphI         2657  GCATGC               GCATG^C
SspBI        3552  TGTACA               T^GTACA
StuI          935  AGGCCT               AGG^CCT
Tth111I      2878  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2274  CCATTGACCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         156  CCCGGG               C^CCGGG
XmaI          156  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccII          38  CGCG                 CG^CG
             3309  CGCG                
AclI         2079  AACGTT               AA^CGTT
             2259  AACGTT              
AlwNI         628  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1566  CAGTGACTG           
AseI         1014  ATTAAT               AT^TAAT
             1709  ATTAAT              
AsnI         1014  ATTAAT               AT^TAAT
             1709  ATTAAT              
AvaI          156  CCCGGG               C^[CT]CG[GA]G
              442  CTCGGG              
BbeI           61  GGCGCC               GGCGC^C
              162  GGCGCC              
BsgI         2004  CTGCAC               GTGCAG(16/14)
             1641  GTGCAG              
BsoBI         156  CCCGGG               C^[CT]CG[GA]G
              442  CTCGGG              
BspMI         379  ACCTGC               ACCTGC(4/8)
              671  ACCTGC              
BssSI         388  CACGAG               CACGAG(-5/-1)
             2567  CACGAG              
BstEII       1113  GGTTACC              G^GT[ACGT]ACC
             2269  GGTGACC             
BstPI        1113  GGTTACC              G^GT[ACGT]ACC
             2269  GGTGACC             
BstUI          38  CGCG                 CG^CG
             3309  CGCG                
BstXI         574  CCAATGGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2524  CCACAGGGCTGG        
BstYI        2707  GGATCT               [GA]^GATC[CT]
             3159  GGATCC              
DsaI          208  CCGTGG               C^C[GA][CT]GG
             1627  CCATGG              
EaeI          238  TGGCCA               [CT]^GGCC[GA]
              271  CGGCCG              
EcoO65I      1113  GGTTACC              G^GT[ACGT]ACC
             2269  GGTGACC             
EcoT22I       708  ATGCAT               ATGCA^T
             2655  ATGCAT              
HindIII       279  AAGCTT               A^AGCTT
             2831  AAGCTT              
KasI           61  GGCGCC               G^GCGCC
              162  GGCGCC              
MflI         2707  GGATCT               [GA]^GATC[CT]
             3159  GGATCC              
MslI         1846  CATCCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2503  CATAAAAATG          
MvnI           38  CGCG                 CG^CG
             3309  CGCG                
NarI           61  GGCGCC               GG^CGCC
              162  GGCGCC              
NsiI          708  ATGCAT               ATGCA^T
             2655  ATGCAT              
PflMI        1092  CCATCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1565  CCAGTGACTGG         
PshBI        1014  ATTAAT               AT^TAAT
             1709  ATTAAT              
Psp1406I     2079  AACGTT               AA^CGTT
             2259  AACGTT              
PvuII        1183  CAGCTG               CAG^CTG
             1644  CAGCTG              
SacI         1981  GAGCTC               GAGCT^C
             3079  GAGCTC              
SexAI         328  ACCTGGT              A^CC[AT]GGT
             1450  ACCAGGT             
SfoI           61  GGCGCC               GGC^GCC
              162  GGCGCC              
SspI         3455  AATATT               AAT^ATT
             3626  AATATT              
TaqI         1496  TCGA                 T^CGA
             2713  TCGA                
TthHB8I      1496  TCGA                 T^CGA
             2713  TCGA                
Van91I       1092  CCATCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1565  CCAGTGACTGG         
XhoII        2707  GGATCT               [GA]^GATC[CT]
             3159  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BanI        
BanII       BbvI        BciVI       BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XbaI