##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoT22I FseI FspI HgaI
HindIII KpnI KspI MamI MluI
MroI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 2049 GGCGCC G[GA]^CG[CT]C
Alw44I 3016 GTGCAC G^TGCAC
AocI 1154 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2126 AGCGCT AGC^GCT
ApaI 837 GGGCCC GGGCC^C
ApaLI 3016 GTGCAC G^TGCAC
BalI 676 TGGCCA TGG^CCA
BbeI 2049 GGCGCC GGCGC^C
BclI 2611 TGATCA T^GATCA
BglI 1948 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 2049 GGCGCC G[GA]^CG[CT]C
BsaWI 1723 TCCGGT [AT]^CCGG[AT]
BsgI 1940 GTGCAG GTGCAG(16/14)
BsmBI 1896 GAGACG CGTCTC(1/5)
BspDI 445 ATCGAT AT^CGAT
BsrFI 2931 ACCGGC [GA]^CCGG[CT]
BstAPI 3347 GCATTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1154 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2931 ACCGGC [GA]^CCGG[CT]
ClaI 445 ATCGAT AT^CGAT
DraI 624 TTTAAA TTT^AAA
DraIII 755 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 676 TGGCCA [CT]^GGCC[GA]
Eco47III 2126 AGCGCT AGC^GCT
Eco81I 1154 CCTGAGG CC^T[ACGT]AGG
FauI 2008 CCCGC CCCGC(4/6)
FbaI 2611 TGATCA T^GATCA
Hin1I 2049 GGCGCC G[GA]^CG[CT]C
HincII 576 GTTAAC GT[CT]^[GA]AC
HindII 576 GTTAAC GT[CT]^[GA]AC
HpaI 576 GTTAAC GTT^AAC
KasI 2049 GGCGCC G^GCGCC
MfeI 2498 CAATTG C^AATTG
MluNI 676 TGGCCA TGG^CCA
MscI 676 TGGCCA TGG^CCA
MunI 2498 CAATTG C^AATTG
NarI 2049 GGCGCC GG^CGCC
NcoI 590 CCATGG C^CATGG
NspI 3471 GCATGC [GA]CATG^[CT]
PpuMI 2800 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 3151 GACCGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2351 CAGCTG CAG^CTG
SapI 1793 GCTCTTC GCTCTTC(1/4)
SexAI 1587 ACCTGGT A^CC[AT]GGT
SfoI 2049 GGCGCC GGC^GCC
SpeI 2135 ACTAGT A^CTAGT
SphI 3471 GCATGC GCATG^C
SspI 3513 AATATT AAT^ATT
XbaI 494 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BamHI 538 GGATCC G^GATCC
1720 GGATCC
BbsI 921 GAAGAC GAAGAC(2/6)
1328 GAAGAC
BpuAI 921 GAAGAC GAAGAC(2/6)
1328 GAAGAC
BspMI 798 GCAGGT ACCTGC(4/8)
2024 GCAGGT
CfoI 2050 GCGC GCG^C
2127 GCGC
DsaI 590 CCATGG C^C[GA][CT]GG
1917 CCGTGG
EcoRV 1062 GATATC GAT^ATC
2951 GATATC
HaeII 2049 GGCGCC [GA]GCGC^[CT]
2126 AGCGCT
HhaI 2050 GCGC GCG^C
2127 GCGC
HinP1I 2050 GCGC G^CGC
2127 GCGC
MspA1I 2210 CCGCTG C[AC]G^C[GT]G
2351 CAGCTG
NheI 2460 GCTAGC G^CTAGC
3082 GCTAGC
SacI 30 GAGCTC GAGCT^C
2700 GAGCTC
StuI 138 AGGCCT AGG^CCT
2940 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BciVI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII