################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AgeI AocI AscI AspI AviII BamHI BbeI BbrPI BbvCI BciVI BclI BglI BglII BsaAI BsaBI BsiWI Bsp1407I BspDI BspMI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI Eco81I EcoNI EcoO65I FbaI FseI FspI KasI MamI MluI NaeI NarI NdeI NgoMIV NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 1967 GGTACC G^GTACC AccI 1510 GTCTAC GT^[AC][GT]AC AccIII 2932 TCCGGA T^CCGGA AclI 374 AACGTT AA^CGTT AcyI 3225 GGCGTC G[GA]^CG[CT]C AflII 1888 CTTAAG C^TTAAG AflIII 1954 ACATGT A^C[GA][CT]GT AhdI 3455 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2123 GTGCAC G^TGCAC Aor51HI 1434 AGCGCT AGC^GCT ApaI 719 GGGCCC GGGCC^C ApaLI 2123 GTGCAC G^TGCAC AseI 1204 ATTAAT AT^TAAT AsnI 1204 ATTAAT AT^TAAT Asp718I 1967 GGTACC G^GTACC AspEI 3455 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3403 CCTAGG C^CTAGG BalI 849 TGGCCA TGG^CCA BanI 1967 GGTACC G^G[CT][GA]CC BfrI 1888 CTTAAG C^TTAAG BlnI 3403 CCTAGG C^CTAGG BlpI 450 GCTTAGC GC^T[ACGT]AGC Bpu1102I 450 GCTTAGC GC^T[ACGT]AGC BsaHI 3225 GGCGTC G[GA]^CG[CT]C BseAI 2932 TCCGGA T^CCGGA BsmBI 3218 CGTCTC CGTCTC(1/5) BspEI 2932 TCCGGA T^CCGGA BspLU11I 1954 ACATGT A^CATGT BsrBI 3206 CCGCTC CCGCTC(-3/-3) BssHII 502 GCGCGC G^CGCGC BssSI 2243 CTCGTG CACGAG(-5/-1) BstXI 530 CCAAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 474 GGATCT [GA]^GATC[CT] CelII 450 GCTTAGC GC^T[ACGT]AGC DraI 3011 TTTAAA TTT^AAA DraIII 2356 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3455 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1434 AGCGCT AGC^GCT EcoRI 643 GAATTC G^AATTC EcoRV 2415 GATATC GAT^ATC EcoT22I 1539 ATGCAT ATGCA^T HaeII 1434 AGCGCT [GA]GCGC^[CT] Hin1I 3225 GGCGTC G[GA]^CG[CT]C KpnI 1967 GGTACC GGTAC^C KspI 108 CCGCGG CCGC^GG MfeI 2659 CAATTG C^AATTG MflI 474 GGATCT [GA]^GATC[CT] MluNI 849 TGGCCA TGG^CCA MroI 2932 TCCGGA T^CCGGA MscI 849 TGGCCA TGG^CCA MunI 2659 CAATTG C^AATTG NcoI 912 CCATGG C^CATGG NheI 3157 GCTAGC G^CTAGC NotI 179 GCGGCCGC GC^GGCCGC NsiI 1539 ATGCAT ATGCA^T PshAI 2053 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1204 ATTAAT AT^TAAT Psp1406I 374 AACGTT AA^CGTT SacII 108 CCGCGG CCGC^GG SpeI 2639 ACTAGT A^CTAGT SspI 1153 AATATT AAT^ATT XcmI 2162 CCAGTGACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 474 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbsI 3174 GAAGAC GAAGAC(2/6) 2694 GTCTTC BcgI 832 CGAGCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 541 GCAAGCAAATCG BpmI 3209 CTCCAG CTGGAG(16/14) 420 CTGGAG BpuAI 3174 GAAGAC GAAGAC(2/6) 2694 GTCTTC BsaI 2299 GAGACC GGTCTC(1/5) 361 GGTCTC BsaWI 248 ACCGGA [AT]^CCGG[AT] 2932 TCCGGA BsmI 314 GAATGC GAATGC(1/-1) 1427 GAATGC BsrFI 2840 ACCGGC [GA]^CCGG[CT] 2984 GCCGGT BstAPI 61 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1709 GCATACTGTGC BstNI 204 CCAGG CC^[AT]GG 2678 CCTGG Cfr10I 2840 ACCGGC [GA]^CCGG[CT] 2984 GCCGGT DraII 718 GGGGCCC [GA]G^G[ACGT]CC[CT] 719 GGGCCCC EcoO109I 718 GGGGCCC [GA]G^G[ACGT]CC[CT] 719 GGGCCCC EcoRII 204 CCAGG ^CC[AT]GG 2678 CCTGG HgaI 3214 GCGTC GACGC(5/10) 3226 GCGTC HindIII 448 AAGCTT A^AGCTT 3005 AAGCTT HphI 527 TCACC GGTGA(8/7) 3552 TCACC MvaI 204 CCAGG CC^[AT]GG 2678 CCTGG PvuII 62 CAGCTG CAG^CTG 1766 CAGCTG SmlI 1888 CTTAAG C^T[CT][GA]AG 3002 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EagI EarI EclXI Eco52I EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XmaCI XmaI XmnI