##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AgeI AocI AscI AspI
AviII BamHI BbeI BbrPI BbvCI
BciVI BclI BglI BglII BsaAI
BsaBI BsiWI Bsp1407I BspDI BspMI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DrdI
Eco81I EcoNI EcoO65I FbaI FseI
FspI KasI MamI MluI NaeI
NarI NdeI NgoMIV NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 1967 GGTACC G^GTACC
AccI 1510 GTCTAC GT^[AC][GT]AC
AccIII 2932 TCCGGA T^CCGGA
AclI 374 AACGTT AA^CGTT
AcyI 3225 GGCGTC G[GA]^CG[CT]C
AflII 1888 CTTAAG C^TTAAG
AflIII 1954 ACATGT A^C[GA][CT]GT
AhdI 3455 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2123 GTGCAC G^TGCAC
Aor51HI 1434 AGCGCT AGC^GCT
ApaI 719 GGGCCC GGGCC^C
ApaLI 2123 GTGCAC G^TGCAC
AseI 1204 ATTAAT AT^TAAT
AsnI 1204 ATTAAT AT^TAAT
Asp718I 1967 GGTACC G^GTACC
AspEI 3455 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3403 CCTAGG C^CTAGG
BalI 849 TGGCCA TGG^CCA
BanI 1967 GGTACC G^G[CT][GA]CC
BfrI 1888 CTTAAG C^TTAAG
BlnI 3403 CCTAGG C^CTAGG
BlpI 450 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 450 GCTTAGC GC^T[ACGT]AGC
BsaHI 3225 GGCGTC G[GA]^CG[CT]C
BseAI 2932 TCCGGA T^CCGGA
BsmBI 3218 CGTCTC CGTCTC(1/5)
BspEI 2932 TCCGGA T^CCGGA
BspLU11I 1954 ACATGT A^CATGT
BsrBI 3206 CCGCTC CCGCTC(-3/-3)
BssHII 502 GCGCGC G^CGCGC
BssSI 2243 CTCGTG CACGAG(-5/-1)
BstXI 530 CCAAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 474 GGATCT [GA]^GATC[CT]
CelII 450 GCTTAGC GC^T[ACGT]AGC
DraI 3011 TTTAAA TTT^AAA
DraIII 2356 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3455 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1434 AGCGCT AGC^GCT
EcoRI 643 GAATTC G^AATTC
EcoRV 2415 GATATC GAT^ATC
EcoT22I 1539 ATGCAT ATGCA^T
HaeII 1434 AGCGCT [GA]GCGC^[CT]
Hin1I 3225 GGCGTC G[GA]^CG[CT]C
KpnI 1967 GGTACC GGTAC^C
KspI 108 CCGCGG CCGC^GG
MfeI 2659 CAATTG C^AATTG
MflI 474 GGATCT [GA]^GATC[CT]
MluNI 849 TGGCCA TGG^CCA
MroI 2932 TCCGGA T^CCGGA
MscI 849 TGGCCA TGG^CCA
MunI 2659 CAATTG C^AATTG
NcoI 912 CCATGG C^CATGG
NheI 3157 GCTAGC G^CTAGC
NotI 179 GCGGCCGC GC^GGCCGC
NsiI 1539 ATGCAT ATGCA^T
PshAI 2053 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1204 ATTAAT AT^TAAT
Psp1406I 374 AACGTT AA^CGTT
SacII 108 CCGCGG CCGC^GG
SpeI 2639 ACTAGT A^CTAGT
SspI 1153 AATATT AAT^ATT
XcmI 2162 CCAGTGACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 474 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbsI 3174 GAAGAC GAAGAC(2/6)
2694 GTCTTC
BcgI 832 CGAGCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
541 GCAAGCAAATCG
BpmI 3209 CTCCAG CTGGAG(16/14)
420 CTGGAG
BpuAI 3174 GAAGAC GAAGAC(2/6)
2694 GTCTTC
BsaI 2299 GAGACC GGTCTC(1/5)
361 GGTCTC
BsaWI 248 ACCGGA [AT]^CCGG[AT]
2932 TCCGGA
BsmI 314 GAATGC GAATGC(1/-1)
1427 GAATGC
BsrFI 2840 ACCGGC [GA]^CCGG[CT]
2984 GCCGGT
BstAPI 61 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1709 GCATACTGTGC
BstNI 204 CCAGG CC^[AT]GG
2678 CCTGG
Cfr10I 2840 ACCGGC [GA]^CCGG[CT]
2984 GCCGGT
DraII 718 GGGGCCC [GA]G^G[ACGT]CC[CT]
719 GGGCCCC
EcoO109I 718 GGGGCCC [GA]G^G[ACGT]CC[CT]
719 GGGCCCC
EcoRII 204 CCAGG ^CC[AT]GG
2678 CCTGG
HgaI 3214 GCGTC GACGC(5/10)
3226 GCGTC
HindIII 448 AAGCTT A^AGCTT
3005 AAGCTT
HphI 527 TCACC GGTGA(8/7)
3552 TCACC
MvaI 204 CCAGG CC^[AT]GG
2678 CCTGG
PvuII 62 CAGCTG CAG^CTG
1766 CAGCTG
SmlI 1888 CTTAAG C^T[CT][GA]AG
3002 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EagI EarI EclXI Eco52I
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XmaCI XmaI
XmnI