################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI Alw44I AocI Aor51HI ApaLI AscI Asp718I AviII AvrII BamHI BbeI BbrPI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI BstAPI BstEII BstPI BstYI Bsu36I CelII Cfr10I CpoI DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI FspI Hin1I HpaI KasI KpnI KspI MaeII MflI MroI NaeI NarI NcoI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflIII 2749 ACGCGT A^C[GA][CT]GT AhdI 546 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 681 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 2774 GGGCCC GGGCC^C AseI 3305 ATTAAT AT^TAAT AsnI 3305 ATTAAT AT^TAAT AspEI 546 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 785 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 113 CCCGAG C^[CT]CG[GA]G BanI 3358 GGTGCC G^G[CT][GA]CC BbvCI 1163 GCTGAGG CCTCAGC(-5/-2) BclI 1123 TGATCA T^GATCA BcnI 104 CCCGG CC^[GC]GG BsaBI 671 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 106 CGGTCG CG[GA][CT]^CG BsoBI 113 CCCGAG C^[CT]CG[GA]G BspDI 3093 ATCGAT AT^CGAT Bst1107I 1024 GTATAC GTA^TAC BstBI 1587 TTCGAA TT^CGAA BstXI 2073 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1024 GTATAC GTA^TAC ClaI 3093 ATCGAT AT^CGAT DraIII 1215 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 546 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 2225 CCAAGG C^C[AT][AT]GG EcoT22I 202 ATGCAT ATGCA^T FbaI 1123 TGATCA T^GATCA HaeII 127 AGCGCC [GA]GCGC^[CT] HapII 105 CCGG C^CGG HpaII 105 CCGG C^CGG MamI 671 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2714 CAATTG C^AATTG MluI 2749 ACGCGT A^CGCGT MspI 105 CCGG C^CGG MunI 2714 CAATTG C^AATTG NciI 104 CCCGG CC^[GC]GG NdeI 1860 CATATG CA^TATG NheI 571 GCTAGC G^CTAGC NsiI 202 ATGCAT ATGCA^T NspV 1587 TTCGAA TT^CGAA PflFI 785 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1148 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1947 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3305 ATTAAT AT^TAAT SalI 1944 GTCGAC G^TCGAC ScaI 1427 AGTACT AGT^ACT SfuI 1587 TTCGAA TT^CGAA SspI 2877 AATATT AAT^ATT StyI 2225 CCAAGG C^C[AT][AT]GG SwaI 2817 ATTTAAAT ATTT^AAAT Tth111I 785 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1148 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BalI 559 TGGCCA TGG^CCA 1228 TGGCCA BbsI 79 GTCTTC GAAGAC(2/6) 1584 GTCTTC BpuAI 79 GTCTTC GAAGAC(2/6) 1584 GTCTTC BsiYI 1007 CCCAGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1148 CCACTAGATGG BslI 1007 CCCAGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1148 CCACTAGATGG Bsp1407I 1836 TGTACA T^GTACA 2514 TGTACA BspMI 796 GCAGGT ACCTGC(4/8) 1559 GCAGGT BsrGI 1836 TGTACA T^GTACA 2514 TGTACA EaeI 559 TGGCCA [CT]^GGCC[GA] 1228 TGGCCA HgaI 902 GACGC GACGC(5/10) 40 GCGTC HphI 1418 TCACC GGTGA(8/7) 2222 TCACC MluNI 559 TGGCCA TGG^CCA 1228 TGGCCA MscI 559 TGGCCA TGG^CCA 1228 TGGCCA MslI 1718 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2074 CATGAATGTG MspA1I 1119 CAGCTG C[AC]G^C[GT]G 3054 CAGCTG NspI 2508 ACATGC [GA]CATG^[CT] 2866 ACATGC PvuII 1119 CAGCTG CAG^CTG 3054 CAGCTG SacI 1085 GAGCTC GAGCT^C 1414 GAGCTC SspBI 1836 TGTACA T^GTACA 2514 TGTACA XbaI 821 TCTAGA T^CTAGA 1480 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AluI AlwI ApoI Asp700I AspHI AvaII BanII BbvI BfaI BfrI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HindIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI