##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI BstAPI BstEII
BstPI BstYI Bsu36I CelII Cfr10I
CpoI DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI FspI Hin1I
HpaI KasI KpnI KspI MaeII
MflI MroI NaeI NarI NcoI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflIII 2749 ACGCGT A^C[GA][CT]GT
AhdI 546 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 681 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2774 GGGCCC GGGCC^C
AseI 3305 ATTAAT AT^TAAT
AsnI 3305 ATTAAT AT^TAAT
AspEI 546 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 785 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 113 CCCGAG C^[CT]CG[GA]G
BanI 3358 GGTGCC G^G[CT][GA]CC
BbvCI 1163 GCTGAGG CCTCAGC(-5/-2)
BclI 1123 TGATCA T^GATCA
BcnI 104 CCCGG CC^[GC]GG
BsaBI 671 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 106 CGGTCG CG[GA][CT]^CG
BsoBI 113 CCCGAG C^[CT]CG[GA]G
BspDI 3093 ATCGAT AT^CGAT
Bst1107I 1024 GTATAC GTA^TAC
BstBI 1587 TTCGAA TT^CGAA
BstXI 2073 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1024 GTATAC GTA^TAC
ClaI 3093 ATCGAT AT^CGAT
DraIII 1215 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 546 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 2225 CCAAGG C^C[AT][AT]GG
EcoT22I 202 ATGCAT ATGCA^T
FbaI 1123 TGATCA T^GATCA
HaeII 127 AGCGCC [GA]GCGC^[CT]
HapII 105 CCGG C^CGG
HpaII 105 CCGG C^CGG
MamI 671 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2714 CAATTG C^AATTG
MluI 2749 ACGCGT A^CGCGT
MspI 105 CCGG C^CGG
MunI 2714 CAATTG C^AATTG
NciI 104 CCCGG CC^[GC]GG
NdeI 1860 CATATG CA^TATG
NheI 571 GCTAGC G^CTAGC
NsiI 202 ATGCAT ATGCA^T
NspV 1587 TTCGAA TT^CGAA
PflFI 785 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1148 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1947 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3305 ATTAAT AT^TAAT
SalI 1944 GTCGAC G^TCGAC
ScaI 1427 AGTACT AGT^ACT
SfuI 1587 TTCGAA TT^CGAA
SspI 2877 AATATT AAT^ATT
StyI 2225 CCAAGG C^C[AT][AT]GG
SwaI 2817 ATTTAAAT ATTT^AAAT
Tth111I 785 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1148 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 559 TGGCCA TGG^CCA
1228 TGGCCA
BbsI 79 GTCTTC GAAGAC(2/6)
1584 GTCTTC
BpuAI 79 GTCTTC GAAGAC(2/6)
1584 GTCTTC
BsiYI 1007 CCCAGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1148 CCACTAGATGG
BslI 1007 CCCAGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1148 CCACTAGATGG
Bsp1407I 1836 TGTACA T^GTACA
2514 TGTACA
BspMI 796 GCAGGT ACCTGC(4/8)
1559 GCAGGT
BsrGI 1836 TGTACA T^GTACA
2514 TGTACA
EaeI 559 TGGCCA [CT]^GGCC[GA]
1228 TGGCCA
HgaI 902 GACGC GACGC(5/10)
40 GCGTC
HphI 1418 TCACC GGTGA(8/7)
2222 TCACC
MluNI 559 TGGCCA TGG^CCA
1228 TGGCCA
MscI 559 TGGCCA TGG^CCA
1228 TGGCCA
MslI 1718 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2074 CATGAATGTG
MspA1I 1119 CAGCTG C[AC]G^C[GT]G
3054 CAGCTG
NspI 2508 ACATGC [GA]CATG^[CT]
2866 ACATGC
PvuII 1119 CAGCTG CAG^CTG
3054 CAGCTG
SacI 1085 GAGCTC GAGCT^C
1414 GAGCTC
SspBI 1836 TGTACA T^GTACA
2514 TGTACA
XbaI 821 TCTAGA T^CTAGA
1480 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI ApoI Asp700I
AspHI AvaII BanII BbvI BfaI
BfrI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HindIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmnI