##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        Asp718I     AviII       AvrII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BssHII      BssSI       BstAPI      BstEII      
BstPI       BstYI       Bsu36I      CelII       Cfr10I      
CpoI        DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FseI        FspI        Hin1I       
HpaI        KasI        KpnI        KspI        MaeII       
MflI        MroI        NaeI        NarI        NcoI        
NgoMIV      NotI        NruI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SapI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AflIII       2749  ACGCGT               A^C[GA][CT]GT
AhdI          546  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         681  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2774  GGGCCC               GGGCC^C
AseI         3305  ATTAAT               AT^TAAT
AsnI         3305  ATTAAT               AT^TAAT
AspEI         546  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          785  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          113  CCCGAG               C^[CT]CG[GA]G
BanI         3358  GGTGCC               G^G[CT][GA]CC
BbvCI        1163  GCTGAGG              CCTCAGC(-5/-2)
BclI         1123  TGATCA               T^GATCA
BcnI          104  CCCGG                CC^[GC]GG
BsaBI         671  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         106  CGGTCG               CG[GA][CT]^CG
BsoBI         113  CCCGAG               C^[CT]CG[GA]G
BspDI        3093  ATCGAT               AT^CGAT
Bst1107I     1024  GTATAC               GTA^TAC
BstBI        1587  TTCGAA               TT^CGAA
BstXI        2073  CCATGAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1024  GTATAC               GTA^TAC
ClaI         3093  ATCGAT               AT^CGAT
DraIII       1215  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      546  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I      2225  CCAAGG               C^C[AT][AT]GG
EcoT22I       202  ATGCAT               ATGCA^T
FbaI         1123  TGATCA               T^GATCA
HaeII         127  AGCGCC               [GA]GCGC^[CT]
HapII         105  CCGG                 C^CGG
HpaII         105  CCGG                 C^CGG
MamI          671  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2714  CAATTG               C^AATTG
MluI         2749  ACGCGT               A^CGCGT
MspI          105  CCGG                 C^CGG
MunI         2714  CAATTG               C^AATTG
NciI          104  CCCGG                CC^[GC]GG
NdeI         1860  CATATG               CA^TATG
NheI          571  GCTAGC               G^CTAGC
NsiI          202  ATGCAT               ATGCA^T
NspV         1587  TTCGAA               TT^CGAA
PflFI         785  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1148  CCACTAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1947  GACACCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3305  ATTAAT               AT^TAAT
SalI         1944  GTCGAC               G^TCGAC
ScaI         1427  AGTACT               AGT^ACT
SfuI         1587  TTCGAA               TT^CGAA
SspI         2877  AATATT               AAT^ATT
StyI         2225  CCAAGG               C^C[AT][AT]GG
SwaI         2817  ATTTAAAT             ATTT^AAAT
Tth111I       785  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1148  CCACTAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BalI          559  TGGCCA               TGG^CCA
             1228  TGGCCA              
BbsI           79  GTCTTC               GAAGAC(2/6)
             1584  GTCTTC              
BpuAI          79  GTCTTC               GAAGAC(2/6)
             1584  GTCTTC              
BsiYI        1007  CCCAGCAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1148  CCACTAGATGG         
BslI         1007  CCCAGCAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1148  CCACTAGATGG         
Bsp1407I     1836  TGTACA               T^GTACA
             2514  TGTACA              
BspMI         796  GCAGGT               ACCTGC(4/8)
             1559  GCAGGT              
BsrGI        1836  TGTACA               T^GTACA
             2514  TGTACA              
EaeI          559  TGGCCA               [CT]^GGCC[GA]
             1228  TGGCCA              
HgaI          902  GACGC                GACGC(5/10)
               40  GCGTC               
HphI         1418  TCACC                GGTGA(8/7)
             2222  TCACC               
MluNI         559  TGGCCA               TGG^CCA
             1228  TGGCCA              
MscI          559  TGGCCA               TGG^CCA
             1228  TGGCCA              
MslI         1718  CACTCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2074  CATGAATGTG          
MspA1I       1119  CAGCTG               C[AC]G^C[GT]G
             3054  CAGCTG              
NspI         2508  ACATGC               [GA]CATG^[CT]
             2866  ACATGC              
PvuII        1119  CAGCTG               CAG^CTG
             3054  CAGCTG              
SacI         1085  GAGCTC               GAGCT^C
             1414  GAGCTC              
SspBI        1836  TGTACA               T^GTACA
             2514  TGTACA              
XbaI          821  TCTAGA               T^CTAGA
             1480  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflII       AluI        AlwI        ApoI        Asp700I     
AspHI       AvaII       BanII       BbvI        BfaI        
BfrI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsmAI       BsmFI       BsmI        
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XmnI