##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AvrII BamHI BbeI BbrPI
BclI BfrI BglI BlnI BsaAI
BseAI BspDI BspEI BsrFI BssHII
BstBI Cfr10I ClaI CpoI DraIII
DrdI Eam1105I EcoRV EcoT22I FbaI
FseI HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI SwaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 1280 GTATAC GT^[AC][GT]AC
AccII 69 CGCG CG^CG
AclI 1311 AACGTT AA^CGTT
AcyI 1591 GACGCC G[GA]^CG[CT]C
AflIII 759 ACATGT A^C[GA][CT]GT
Aor51HI 1450 AGCGCT AGC^GCT
ApaI 1627 GGGCCC GGGCC^C
AseI 611 ATTAAT AT^TAAT
AsnI 611 ATTAAT AT^TAAT
Asp700I 2414 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1552 TGCGCA TGC^GCA
BbsI 1788 GAAGAC GAAGAC(2/6)
BbvCI 2120 CCTCAGC CCTCAGC(-5/-2)
BciVI 3335 GTATCC GTATCC(6/5)
BpuAI 1788 GAAGAC GAAGAC(2/6)
BsaBI 78 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1591 GACGCC G[GA]^CG[CT]C
BsiEI 226 CGGCCG CG[GA][CT]^CG
BsiWI 2926 CGTACG C^GTACG
BsmBI 1589 GAGACG CGTCTC(1/5)
Bsp1407I 3363 TGTACA T^GTACA
BspHI 3143 TCATGA T^CATGA
BspLU11I 759 ACATGT A^CATGT
BsrDI 3369 GCAATG GCAATG(2/0)
BsrGI 3363 TGTACA T^GTACA
Bst1107I 1280 GTATAC GTA^TAC
BstEII 1012 GGTGACC G^GT[ACGT]ACC
BstPI 1012 GGTGACC G^GT[ACGT]ACC
BstUI 69 CGCG CG^CG
BstZ17I 1280 GTATAC GTA^TAC
EagI 226 CGGCCG C^GGCCG
EarI 975 GAAGAG CTCTTC(1/4)
EclXI 226 CGGCCG C^GGCCG
Eco47III 1450 AGCGCT AGC^GCT
Eco52I 226 CGGCCG C^GGCCG
EcoO65I 1012 GGTGACC G^GT[ACGT]ACC
EcoRI 1719 GAATTC G^AATTC
FspI 1552 TGCGCA TGC^GCA
Hin1I 1591 GACGCC G[GA]^CG[CT]C
HincII 2756 GTTGAC GT[CT]^[GA]AC
HindII 2756 GTTGAC GT[CT]^[GA]AC
HindIII 917 AAGCTT A^AGCTT
Ksp632I 975 GAAGAG CTCTTC(1/4)
MamI 78 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 69 CGCG CG^CG
NcoI 1651 CCATGG C^CATGG
PacI 3320 TTAATTAA TTAAT^TAA
PaeR7I 2331 CTCGAG C^TCGAG
PpuMI 156 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 358 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 611 ATTAAT AT^TAAT
Psp1406I 1311 AACGTT AA^CGTT
RcaI 3143 TCATGA T^CATGA
SexAI 3393 ACCAGGT A^CC[AT]GGT
SmaI 1711 CCCGGG CCC^GGG
SpeI 11 ACTAGT A^CTAGT
SspBI 3363 TGTACA T^GTACA
XbaI 2213 TCTAGA T^CTAGA
XhoI 2331 CTCGAG C^TCGAG
XmaCI 1711 CCCGGG C^CCGGG
XmaI 1711 CCCGGG C^CCGGG
XmnI 2414 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 1956 CCTGAGG CC^T[ACGT]AGG
2491 CCTCAGG
BalI 306 TGGCCA TGG^CCA
2726 TGGCCA
BanI 1794 GGTGCC G^G[CT][GA]CC
1821 GGTGCC
BcgI 2992 CGACCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1179 GCACTGTTCTCG
BlpI 392 GCTTAGC GC^T[ACGT]AGC
1492 GCTCAGC
Bpu1102I 392 GCTTAGC GC^T[ACGT]AGC
1492 GCTCAGC
BsaI 2544 GGTCTC GGTCTC(1/5)
3122 GGTCTC
BsaWI 1730 ACCGGA [AT]^CCGG[AT]
2140 ACCGGA
BsmI 258 GAATGC GAATGC(1/-1)
3217 GAATGC
BstAPI 1634 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2851 GCAAAATCTGC
BstXI 1021 CCAGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1850 CCAGTCAGATGG
Bsu36I 1956 CCTGAGG CC^T[ACGT]AGG
2491 CCTCAGG
CelII 392 GCTTAGC GC^T[ACGT]AGC
1492 GCTCAGC
DraII 156 GGGTCCT [GA]G^G[ACGT]CC[CT]
2341 AGGCCCC
DsaI 1651 CCATGG C^C[GA][CT]GG
1921 CCGTGG
Eco81I 1956 CCTGAGG CC^T[ACGT]AGG
2491 CCTCAGG
EcoNI 2557 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3226 CCTAAAGTAGG
EcoO109I 156 GGGTCCT [GA]G^G[ACGT]CC[CT]
2341 AGGCCCC
HaeII 1450 AGCGCT [GA]GCGC^[CT]
1636 AGCGCC
MaeII 1312 ACGT A^CGT
2047 ACGT
MluNI 306 TGGCCA TGG^CCA
2726 TGGCCA
MscI 306 TGGCCA TGG^CCA
2726 TGGCCA
SphI 1158 GCATGC GCATG^C
1444 GCATGC
StuI 1252 AGGCCT AGG^CCT
2797 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AspI AvaI
AvaII BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII