################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbrPI BclI BfrI BglII BlnI BsaHI BseAI BsiEI BspDI BspEI BssHII BssSI Bst1107I BstUI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoNI EcoT22I FbaI FseI FspI Hin1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SpeI Sse8387I StuI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AflIII 1750 ACATGT A^C[GA][CT]GT AhdI 1555 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 2849 GATCC GGATC(4/5) AocI 1283 CCTCAGG CC^T[ACGT]AGG Aor51HI 1625 AGCGCT AGC^GCT ApaI 1204 GGGCCC GGGCC^C AspEI 1555 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2555 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1302 TGGCCA TGG^CCA BbsI 3236 GTCTTC GAAGAC(2/6) BbvCI 2366 CCTCAGC CCTCAGC(-5/-2) BcgI 2727 CGACCTACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 3167 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 3236 GTCTTC GAAGAC(2/6) BsaAI 557 TACGTA [CT]AC^GT[GA] BsaBI 2963 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 7 TCCGGT [AT]^CCGG[AT] BsiWI 813 CGTACG C^GTACG BsmI 18 GCATTC GAATGC(1/-1) BspHI 1756 TCATGA T^CATGA BspLU11I 1750 ACATGT A^CATGT BstBI 1435 TTCGAA TT^CGAA BstXI 1312 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2848 AGATCC [GA]^GATC[CT] Bsu36I 1283 CCTCAGG CC^T[ACGT]AGG DraI 1861 TTTAAA TTT^AAA DraIII 326 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1302 TGGCCA [CT]^GGCC[GA] Eam1105I 1555 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1027 GAAGAG CTCTTC(1/4) Eco47III 1625 AGCGCT AGC^GCT Eco81I 1283 CCTCAGG CC^T[ACGT]AGG EcoRV 2149 GATATC GAT^ATC HincII 2242 GTTAAC GT[CT]^[GA]AC HindII 2242 GTTAAC GT[CT]^[GA]AC HpaI 2242 GTTAAC GTT^AAC Ksp632I 1027 GAAGAG CTCTTC(1/4) MaeII 558 ACGT A^CGT MamI 2963 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 2848 AGATCC [GA]^GATC[CT] MluNI 1302 TGGCCA TGG^CCA MscI 1302 TGGCCA TGG^CCA NaeI 2533 GCCGGC GCC^GGC NcoI 2637 CCATGG C^CATGG NgoMIV 2533 GCCGGC G^CCGGC NspV 1435 TTCGAA TT^CGAA PflFI 2555 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1883 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2626 CTGCAG CTGCA^G RcaI 1756 TCATGA T^CATGA SacI 1272 GAGCTC GAGCT^C SexAI 1509 ACCAGGT A^CC[AT]GGT SfuI 1435 TTCGAA TT^CGAA SmaI 2563 CCCGGG CCC^GGG SnaBI 557 TACGTA TAC^GTA SphI 2842 GCATGC GCATG^C TaqI 1436 TCGA T^CGA Tth111I 2555 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1436 TCGA T^CGA XhoII 2848 AGATCC [GA]^GATC[CT] XmaCI 2563 CCCGGG C^CCGGG XmaI 2563 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BanI 334 GGTGCC G^G[CT][GA]CC 2180 GGTGCC BciVI 1825 GGATAC GTATCC(6/5) 2968 GTATCC BsaI 3578 GAGACC GGTCTC(1/5) 3514 GGTCTC Bsp1407I 431 TGTACA T^GTACA 2782 TGTACA BsrBI 1609 GAGCGG CCGCTC(-3/-3) 2658 GAGCGG BsrGI 431 TGTACA T^GTACA 2782 TGTACA BstAPI 1183 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1420 GCAAACCGTGC DsaI 2637 CCATGG C^C[GA][CT]GG 3138 CCGTGG EcoT14I 1492 CCAAGG C^C[AT][AT]GG 2637 CCATGG HgaI 1645 GACGC GACGC(5/10) 2831 GCGTC HindIII 1226 AAGCTT A^AGCTT 3399 AAGCTT SmlI 1885 CTCAAG C^T[CT][GA]AG 3223 CTCAAG SspBI 431 TGTACA T^GTACA 2782 TGTACA StyI 1492 CCAAGG C^C[AT][AT]GG 2637 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaI AvaII BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoO65I EcoRI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI