##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbeI BbrPI
BclI BfrI BglII BlnI BsaHI
BseAI BsiEI BspDI BspEI BssHII
BssSI Bst1107I BstUI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoNI EcoT22I FbaI FseI FspI
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SpeI
Sse8387I StuI SwaI Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AflIII 1750 ACATGT A^C[GA][CT]GT
AhdI 1555 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2849 GATCC GGATC(4/5)
AocI 1283 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1625 AGCGCT AGC^GCT
ApaI 1204 GGGCCC GGGCC^C
AspEI 1555 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2555 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1302 TGGCCA TGG^CCA
BbsI 3236 GTCTTC GAAGAC(2/6)
BbvCI 2366 CCTCAGC CCTCAGC(-5/-2)
BcgI 2727 CGACCTACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3167 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3236 GTCTTC GAAGAC(2/6)
BsaAI 557 TACGTA [CT]AC^GT[GA]
BsaBI 2963 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 7 TCCGGT [AT]^CCGG[AT]
BsiWI 813 CGTACG C^GTACG
BsmI 18 GCATTC GAATGC(1/-1)
BspHI 1756 TCATGA T^CATGA
BspLU11I 1750 ACATGT A^CATGT
BstBI 1435 TTCGAA TT^CGAA
BstXI 1312 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2848 AGATCC [GA]^GATC[CT]
Bsu36I 1283 CCTCAGG CC^T[ACGT]AGG
DraI 1861 TTTAAA TTT^AAA
DraIII 326 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1302 TGGCCA [CT]^GGCC[GA]
Eam1105I 1555 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1027 GAAGAG CTCTTC(1/4)
Eco47III 1625 AGCGCT AGC^GCT
Eco81I 1283 CCTCAGG CC^T[ACGT]AGG
EcoRV 2149 GATATC GAT^ATC
HincII 2242 GTTAAC GT[CT]^[GA]AC
HindII 2242 GTTAAC GT[CT]^[GA]AC
HpaI 2242 GTTAAC GTT^AAC
Ksp632I 1027 GAAGAG CTCTTC(1/4)
MaeII 558 ACGT A^CGT
MamI 2963 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2848 AGATCC [GA]^GATC[CT]
MluNI 1302 TGGCCA TGG^CCA
MscI 1302 TGGCCA TGG^CCA
NaeI 2533 GCCGGC GCC^GGC
NcoI 2637 CCATGG C^CATGG
NgoMIV 2533 GCCGGC G^CCGGC
NspV 1435 TTCGAA TT^CGAA
PflFI 2555 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1883 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2626 CTGCAG CTGCA^G
RcaI 1756 TCATGA T^CATGA
SacI 1272 GAGCTC GAGCT^C
SexAI 1509 ACCAGGT A^CC[AT]GGT
SfuI 1435 TTCGAA TT^CGAA
SmaI 2563 CCCGGG CCC^GGG
SnaBI 557 TACGTA TAC^GTA
SphI 2842 GCATGC GCATG^C
TaqI 1436 TCGA T^CGA
Tth111I 2555 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1436 TCGA T^CGA
XhoII 2848 AGATCC [GA]^GATC[CT]
XmaCI 2563 CCCGGG C^CCGGG
XmaI 2563 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BanI 334 GGTGCC G^G[CT][GA]CC
2180 GGTGCC
BciVI 1825 GGATAC GTATCC(6/5)
2968 GTATCC
BsaI 3578 GAGACC GGTCTC(1/5)
3514 GGTCTC
Bsp1407I 431 TGTACA T^GTACA
2782 TGTACA
BsrBI 1609 GAGCGG CCGCTC(-3/-3)
2658 GAGCGG
BsrGI 431 TGTACA T^GTACA
2782 TGTACA
BstAPI 1183 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1420 GCAAACCGTGC
DsaI 2637 CCATGG C^C[GA][CT]GG
3138 CCGTGG
EcoT14I 1492 CCAAGG C^C[AT][AT]GG
2637 CCATGG
HgaI 1645 GACGC GACGC(5/10)
2831 GCGTC
HindIII 1226 AAGCTT A^AGCTT
3399 AAGCTT
SmlI 1885 CTCAAG C^T[CT][GA]AG
3223 CTCAAG
SspBI 431 TGTACA T^GTACA
2782 TGTACA
StyI 1492 CCAAGG C^C[AT][AT]GG
2637 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwNI ApoI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoO65I EcoRI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI