##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BamHI       BbeI        BbrPI       
BclI        BfrI        BglII       BlnI        BsaHI       
BseAI       BsiEI       BspDI       BspEI       BssHII      
BssSI       Bst1107I    BstUI       BstZ17I     ClaI        
CpoI        DrdI        EagI        EclXI       Eco52I      
EcoNI       EcoT22I     FbaI        FseI        FspI        
Hin1I       KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SpeI        
Sse8387I    StuI        SwaI        Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AflIII       1750  ACATGT               A^C[GA][CT]GT
AhdI         1555  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         2849  GATCC                GGATC(4/5)
AocI         1283  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1625  AGCGCT               AGC^GCT
ApaI         1204  GGGCCC               GGGCC^C
AspEI        1555  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2555  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1302  TGGCCA               TGG^CCA
BbsI         3236  GTCTTC               GAAGAC(2/6)
BbvCI        2366  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2727  CGACCTACATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         3167  GCCCAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        3236  GTCTTC               GAAGAC(2/6)
BsaAI         557  TACGTA               [CT]AC^GT[GA]
BsaBI        2963  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI           7  TCCGGT               [AT]^CCGG[AT]
BsiWI         813  CGTACG               C^GTACG
BsmI           18  GCATTC               GAATGC(1/-1)
BspHI        1756  TCATGA               T^CATGA
BspLU11I     1750  ACATGT               A^CATGT
BstBI        1435  TTCGAA               TT^CGAA
BstXI        1312  CCAAGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        2848  AGATCC               [GA]^GATC[CT]
Bsu36I       1283  CCTCAGG              CC^T[ACGT]AGG
DraI         1861  TTTAAA               TTT^AAA
DraIII        326  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1302  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1555  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1027  GAAGAG               CTCTTC(1/4)
Eco47III     1625  AGCGCT               AGC^GCT
Eco81I       1283  CCTCAGG              CC^T[ACGT]AGG
EcoRV        2149  GATATC               GAT^ATC
HincII       2242  GTTAAC               GT[CT]^[GA]AC
HindII       2242  GTTAAC               GT[CT]^[GA]AC
HpaI         2242  GTTAAC               GTT^AAC
Ksp632I      1027  GAAGAG               CTCTTC(1/4)
MaeII         558  ACGT                 A^CGT
MamI         2963  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         2848  AGATCC               [GA]^GATC[CT]
MluNI        1302  TGGCCA               TGG^CCA
MscI         1302  TGGCCA               TGG^CCA
NaeI         2533  GCCGGC               GCC^GGC
NcoI         2637  CCATGG               C^CATGG
NgoMIV       2533  GCCGGC               G^CCGGC
NspV         1435  TTCGAA               TT^CGAA
PflFI        2555  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1883  GACTCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2626  CTGCAG               CTGCA^G
RcaI         1756  TCATGA               T^CATGA
SacI         1272  GAGCTC               GAGCT^C
SexAI        1509  ACCAGGT              A^CC[AT]GGT
SfuI         1435  TTCGAA               TT^CGAA
SmaI         2563  CCCGGG               CCC^GGG
SnaBI         557  TACGTA               TAC^GTA
SphI         2842  GCATGC               GCATG^C
TaqI         1436  TCGA                 T^CGA
Tth111I      2555  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1436  TCGA                 T^CGA
XhoII        2848  AGATCC               [GA]^GATC[CT]
XmaCI        2563  CCCGGG               C^CCGGG
XmaI         2563  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BanI          334  GGTGCC               G^G[CT][GA]CC
             2180  GGTGCC              
BciVI        1825  GGATAC               GTATCC(6/5)
             2968  GTATCC              
BsaI         3578  GAGACC               GGTCTC(1/5)
             3514  GGTCTC              
Bsp1407I      431  TGTACA               T^GTACA
             2782  TGTACA              
BsrBI        1609  GAGCGG               CCGCTC(-3/-3)
             2658  GAGCGG              
BsrGI         431  TGTACA               T^GTACA
             2782  TGTACA              
BstAPI       1183  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1420  GCAAACCGTGC         
DsaI         2637  CCATGG               C^C[GA][CT]GG
             3138  CCGTGG              
EcoT14I      1492  CCAAGG               C^C[AT][AT]GG
             2637  CCATGG              
HgaI         1645  GACGC                GACGC(5/10)
             2831  GCGTC               
HindIII      1226  AAGCTT               A^AGCTT
             3399  AAGCTT              
SmlI         1885  CTCAAG               C^T[CT][GA]AG
             3223  CTCAAG              
SspBI         431  TGTACA               T^GTACA
             2782  TGTACA              
StyI         1492  CCAAGG               C^C[AT][AT]GG
             2637  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BanII       BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoO65I     EcoRI       EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SspI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XcmI