################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Aor51HI AseI AsnI AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrFI BssSI Bst1107I BstZ17I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI SspI StuI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 625 GGTACC G^GTACC AccI 24 GTCGAC GT^[AC][GT]AC Alw44I 1742 GTGCAC G^TGCAC AlwI 1651 GGATC GGATC(4/5) AocI 1580 CCTGAGG CC^T[ACGT]AGG ApaI 1780 GGGCCC GGGCC^C ApaLI 1742 GTGCAC G^TGCAC AscI 53 GGCGCGCC GG^CGCGCC Asp700I 2022 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 625 GGTACC G^GTACC AspI 914 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 958 CCCGAG C^[CT]CG[GA]G BlpI 1614 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1614 GCTGAGC GC^T[ACGT]AGC BsmBI 538 GAGACG CGTCTC(1/5) BsmI 1343 GCATTC GAATGC(1/-1) BsoBI 958 CCCGAG C^[CT]CG[GA]G Bsp1407I 665 TGTACA T^GTACA BspLU11I 698 ACATGT A^CATGT BsrBI 1441 GAGCGG CCGCTC(-3/-3) BsrDI 2142 CATTGC GCAATG(2/0) BsrGI 665 TGTACA T^GTACA BssHII 54 GCGCGC G^CGCGC BstAPI 1407 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 368 TTCGAA TT^CGAA BstEII 1155 GGTGACC G^GT[ACGT]ACC BstPI 1155 GGTGACC G^GT[ACGT]ACC BstXI 2174 CCACGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1651 GGATCT [GA]^GATC[CT] Bsu36I 1580 CCTGAGG CC^T[ACGT]AGG CelII 1614 GCTGAGC GC^T[ACGT]AGC Eco81I 1580 CCTGAGG CC^T[ACGT]AGG EcoO65I 1155 GGTGACC G^GT[ACGT]ACC HaeII 1709 AGCGCC [GA]GCGC^[CT] HincII 24 GTCGAC GT[CT]^[GA]AC HindII 24 GTCGAC GT[CT]^[GA]AC KpnI 625 GGTACC GGTAC^C MflI 1651 GGATCT [GA]^GATC[CT] NdeI 2503 CATATG CA^TATG NspI 698 ACATGT [GA]CATG^[CT] NspV 368 TTCGAA TT^CGAA PflFI 914 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 505 GAGCTC GAGCT^C SalI 24 GTCGAC G^TCGAC SapI 1386 GAAGAGC GCTCTTC(1/4) SfuI 368 TTCGAA TT^CGAA SpeI 1899 ACTAGT A^CTAGT SspBI 665 TGTACA T^GTACA SwaI 1912 ATTTAAAT ATTT^AAAT Tth111I 914 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1651 GGATCT [GA]^GATC[CT] XmnI 2022 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 1427 AAATTC [GA]^AATT[CT] 2005 AAATTT AcyI 27 GACGCC G[GA]^CG[CT]C 1534 GGCGTC ApoI 1427 AAATTC [GA]^AATT[CT] 2005 AAATTT BfaI 1789 CTAG C^TAG 1900 CTAG BsaHI 27 GACGCC G[GA]^CG[CT]C 1534 GGCGTC BseRI 1051 GAGGAG GAGGAG(10/8) 1583 GAGGAG DpnII 969 GATC ^GATC 1652 GATC DrdI 540 GACGCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2275 GACTTTAGTGTC Hin1I 27 GACGCC G[GA]^CG[CT]C 1534 GGCGTC MaeI 1789 CTAG C^TAG 1900 CTAG MaeII 1801 ACGT A^CGT 2125 ACGT MboI 969 GATC ^GATC 1652 GATC NcoI 14 CCATGG C^CATGG 644 CCATGG NdeII 969 GATC ^GATC 1652 GATC Sau3AI 969 GATC ^GATC 1652 GATC SbfI 682 CCTGCAGG CCTGCA^GG 1713 CCTGCAGG SmlI 442 CTTGAG C^T[CT][GA]AG 2369 CTTGAG Sse8387I 682 CCTGCAGG CCTGCA^GG 1713 CCTGCAGG Tsp45I 608 GTGAC ^GT[GC]AC 1156 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I