##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AseI AsnI AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrFI BssSI Bst1107I BstZ17I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI SspI StuI
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 625 GGTACC G^GTACC
AccI 24 GTCGAC GT^[AC][GT]AC
Alw44I 1742 GTGCAC G^TGCAC
AlwI 1651 GGATC GGATC(4/5)
AocI 1580 CCTGAGG CC^T[ACGT]AGG
ApaI 1780 GGGCCC GGGCC^C
ApaLI 1742 GTGCAC G^TGCAC
AscI 53 GGCGCGCC GG^CGCGCC
Asp700I 2022 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 625 GGTACC G^GTACC
AspI 914 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 958 CCCGAG C^[CT]CG[GA]G
BlpI 1614 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1614 GCTGAGC GC^T[ACGT]AGC
BsmBI 538 GAGACG CGTCTC(1/5)
BsmI 1343 GCATTC GAATGC(1/-1)
BsoBI 958 CCCGAG C^[CT]CG[GA]G
Bsp1407I 665 TGTACA T^GTACA
BspLU11I 698 ACATGT A^CATGT
BsrBI 1441 GAGCGG CCGCTC(-3/-3)
BsrDI 2142 CATTGC GCAATG(2/0)
BsrGI 665 TGTACA T^GTACA
BssHII 54 GCGCGC G^CGCGC
BstAPI 1407 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 368 TTCGAA TT^CGAA
BstEII 1155 GGTGACC G^GT[ACGT]ACC
BstPI 1155 GGTGACC G^GT[ACGT]ACC
BstXI 2174 CCACGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1651 GGATCT [GA]^GATC[CT]
Bsu36I 1580 CCTGAGG CC^T[ACGT]AGG
CelII 1614 GCTGAGC GC^T[ACGT]AGC
Eco81I 1580 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1155 GGTGACC G^GT[ACGT]ACC
HaeII 1709 AGCGCC [GA]GCGC^[CT]
HincII 24 GTCGAC GT[CT]^[GA]AC
HindII 24 GTCGAC GT[CT]^[GA]AC
KpnI 625 GGTACC GGTAC^C
MflI 1651 GGATCT [GA]^GATC[CT]
NdeI 2503 CATATG CA^TATG
NspI 698 ACATGT [GA]CATG^[CT]
NspV 368 TTCGAA TT^CGAA
PflFI 914 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 505 GAGCTC GAGCT^C
SalI 24 GTCGAC G^TCGAC
SapI 1386 GAAGAGC GCTCTTC(1/4)
SfuI 368 TTCGAA TT^CGAA
SpeI 1899 ACTAGT A^CTAGT
SspBI 665 TGTACA T^GTACA
SwaI 1912 ATTTAAAT ATTT^AAAT
Tth111I 914 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1651 GGATCT [GA]^GATC[CT]
XmnI 2022 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1427 AAATTC [GA]^AATT[CT]
2005 AAATTT
AcyI 27 GACGCC G[GA]^CG[CT]C
1534 GGCGTC
ApoI 1427 AAATTC [GA]^AATT[CT]
2005 AAATTT
BfaI 1789 CTAG C^TAG
1900 CTAG
BsaHI 27 GACGCC G[GA]^CG[CT]C
1534 GGCGTC
BseRI 1051 GAGGAG GAGGAG(10/8)
1583 GAGGAG
DpnII 969 GATC ^GATC
1652 GATC
DrdI 540 GACGCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2275 GACTTTAGTGTC
Hin1I 27 GACGCC G[GA]^CG[CT]C
1534 GGCGTC
MaeI 1789 CTAG C^TAG
1900 CTAG
MaeII 1801 ACGT A^CGT
2125 ACGT
MboI 969 GATC ^GATC
1652 GATC
NcoI 14 CCATGG C^CATGG
644 CCATGG
NdeII 969 GATC ^GATC
1652 GATC
Sau3AI 969 GATC ^GATC
1652 GATC
SbfI 682 CCTGCAGG CCTGCA^GG
1713 CCTGCAGG
SmlI 442 CTTGAG C^T[CT][GA]AG
2369 CTTGAG
Sse8387I 682 CCTGCAGG CCTGCA^GG
1713 CCTGCAGG
Tsp45I 608 GTGAC ^GT[GC]AC
1156 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I