##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI ApoI AseI AsnI Asp700I
BbvCI BciVI BfrI BseAI BsiWI
BspDI BspEI BspHI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoRI EcoT22I HpaI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 152 GGTACC G^GTACC
AccI 2255 GTCGAC GT^[AC][GT]AC
AflIII 1922 ACGTGT A^C[GA][CT]GT
AhdI 2868 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 2471 GGCGCGCC GG^CGCGCC
Asp718I 152 GGTACC G^GTACC
AspEI 2868 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3690 GGATCC G^GATCC
BbrPI 1921 CACGTG CAC^GTG
BcgI 1806 CGAGCTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 324 TGATCA T^GATCA
BglI 765 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3704 AGATCT A^GATCT
BsaBI 3699 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 737 GAGACG CGTCTC(1/5)
BsrBI 716 CCGCTC CCGCTC(-3/-3)
BssSI 3474 CTCGTG CACGAG(-5/-1)
BstAPI 1515 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1232 GGTGACC G^GT[ACGT]ACC
BstPI 1232 GGTGACC G^GT[ACGT]ACC
DraIII 2401 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2868 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1232 GGTGACC G^GT[ACGT]ACC
EcoRV 2169 GATATC GAT^ATC
FbaI 324 TGATCA T^GATCA
FseI 3387 GGCCGGCC GGCCGG^CC
HincII 2255 GTCGAC GT[CT]^[GA]AC
HindII 2255 GTCGAC GT[CT]^[GA]AC
HindIII 515 AAGCTT A^AGCTT
KpnI 152 GGTACC GGTAC^C
KspI 437 CCGCGG CCGC^GG
MamI 3699 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3388 GCCGGC GCC^GGC
NcoI 1303 CCATGG C^CATGG
NdeI 3554 CATATG CA^TATG
NgoMIV 3388 GCCGGC G^CCGGC
PmaCI 1921 CACGTG CAC^GTG
PmlI 1921 CACGTG CAC^GTG
SacII 437 CCGCGG CCGC^GG
SalI 2255 GTCGAC G^TCGAC
SexAI 3227 ACCTGGT A^CC[AT]GGT
SphI 541 GCATGC GCATG^C
Tsp509I 3808 AATT ^AATT
XcmI 1025 CCAAGCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AspI 1759 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2718 GACCGCGTC
AviII 1132 TGCGCA TGC^GCA
2512 TGCGCA
AvrII 453 CCTAGG C^CTAGG
3561 CCTAGG
BbeI 521 GGCGCC GGCGC^C
2773 GGCGCC
BlnI 453 CCTAGG C^CTAGG
3561 CCTAGG
BsaAI 1648 TACGTG [CT]AC^GT[GA]
1921 CACGTG
BsiEI 66 CGGCCG CG[GA][CT]^CG
835 CGGCCG
BsmI 1159 GCATTC GAATGC(1/-1)
1219 GCATTC
Bsp1407I 1176 TGTACA T^GTACA
2597 TGTACA
BsrDI 61 CATTGC GCAATG(2/0)
1714 GCAATG
BsrGI 1176 TGTACA T^GTACA
2597 TGTACA
BssHII 895 GCGCGC G^CGCGC
2472 GCGCGC
BstXI 1331 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2565 CCAGATGACTGG
BstYI 3690 GGATCC [GA]^GATC[CT]
3704 AGATCT
DrdI 246 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2246 GACCTCAATGTC
DsaI 437 CCGCGG C^C[GA][CT]GG
1303 CCATGG
EagI 66 CGGCCG C^GGCCG
835 CGGCCG
EclXI 66 CGGCCG C^GGCCG
835 CGGCCG
Eco52I 66 CGGCCG C^GGCCG
835 CGGCCG
EcoNI 3506 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3583 CCTGGGAGAGG
FspI 1132 TGCGCA TGC^GCA
2512 TGCGCA
KasI 521 GGCGCC G^GCGCC
2773 GGCGCC
MflI 3690 GGATCC [GA]^GATC[CT]
3704 AGATCT
MslI 1917 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3177 CATTTGCATG
NarI 521 GGCGCC GG^CGCC
2773 GGCGCC
NspI 541 GCATGC [GA]CATG^[CT]
2585 ACATGC
PflFI 1759 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2718 GACCGCGTC
PvuII 1063 CAGCTG CAG^CTG
3061 CAGCTG
SacI 1488 GAGCTC GAGCT^C
2380 GAGCTC
SapI 3306 GAAGAGC GCTCTTC(1/4)
906 GCTCTTC
SfoI 521 GGCGCC GGC^GCC
2773 GGCGCC
SmlI 2499 CTTGAG C^T[CT][GA]AG
3338 CTTGAG
SspBI 1176 TGTACA T^GTACA
2597 TGTACA
Tth111I 1759 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2718 GACCGCGTC
XhoII 3690 GGATCC [GA]^GATC[CT]
3704 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI Eco47III Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XbaI XmaCI XmaI