##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AclI AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BbvCI BclI BlnI BsaI BsiWI
Bsp1407I BspDI BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRV FbaI FseI
FspI HpaI KspI MfeI MluI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1413 GGTACC G^GTACC
AflII 1289 CTTAAG C^TTAAG
Alw44I 455 GTGCAC G^TGCAC
AlwNI 1249 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 275 AGCGCT AGC^GCT
ApaLI 455 GTGCAC G^TGCAC
Asp700I 2345 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1413 GGTACC G^GTACC
BbeI 97 GGCGCC GGCGC^C
BbrPI 1025 CACGTG CAC^GTG
BciVI 1401 GTATCC GTATCC(6/5)
BfrI 1289 CTTAAG C^TTAAG
BglI 843 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 331 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1509 CGACCG CG[GA][CT]^CG
BsmBI 1853 CGTCTC CGTCTC(1/5)
BsrBI 3466 GAGCGG CCGCTC(-3/-3)
BsrFI 3098 GCCGGT [GA]^CCGG[CT]
Cfr10I 3098 GCCGGT [GA]^CCGG[CT]
DraI 2710 TTTAAA TTT^AAA
DraIII 641 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 656 CCACGG C^C[GA][CT]GG
Eco47III 275 AGCGCT AGC^GCT
EcoRI 2572 GAATTC G^AATTC
EcoT22I 2410 ATGCAT ATGCA^T
HincII 1389 GTCAAC GT[CT]^[GA]AC
HindII 1389 GTCAAC GT[CT]^[GA]AC
HindIII 1933 AAGCTT A^AGCTT
KasI 97 GGCGCC G^GCGCC
KpnI 1413 GGTACC GGTAC^C
MamI 331 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 97 GGCGCC GG^CGCC
NsiI 2410 ATGCAT ATGCA^T
PmaCI 1025 CACGTG CAC^GTG
PmlI 1025 CACGTG CAC^GTG
PpuMI 2598 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3000 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1266 GAGCTC GAGCT^C
ScaI 603 AGTACT AGT^ACT
SexAI 1409 ACCTGGT A^CC[AT]GGT
SfoI 97 GGCGCC GGC^GCC
SmaI 3285 CCCGGG CCC^GGG
SphI 148 GCATGC GCATG^C
StuI 2451 AGGCCT AGG^CCT
XcmI 868 CCATCTGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3285 CCCGGG C^CCGGG
XmaI 3285 CCCGGG C^CCGGG
XmnI 2345 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1743 GACGTC GACGT^C
1851 GACGTC
AccI 2186 GTCTAC GT^[AC][GT]AC
3240 GTAGAC
AccIII 903 TCCGGA T^CCGGA
2664 TCCGGA
AocI 325 CCTCAGG CC^T[ACGT]AGG
1861 CCTCAGG
ApaI 2944 GGGCCC GGGCC^C
3179 GGGCCC
AspI 616 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1579 GACTACGTC
BamHI 1866 GGATCC G^GATCC
3346 GGATCC
BcgI 24 CGAAGCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
395 GCAGAGACATCG
BglII 1080 AGATCT A^GATCT
2798 AGATCT
BsaAI 1025 CACGTG [CT]AC^GT[GA]
1751 TACGTG
BseAI 903 TCCGGA T^CCGGA
2664 TCCGGA
BseRI 1999 GAGGAG GAGGAG(10/8)
3070 GAGGAG
BsmI 2349 GCATTC GAATGC(1/-1)
2925 GCATTC
BspEI 903 TCCGGA T^CCGGA
2664 TCCGGA
BspHI 3159 TCATGA T^CATGA
3251 TCATGA
BspLU11I 785 ACATGT A^CATGT
819 ACATGT
BssSI 3541 CACGAG CACGAG(-5/-1)
2545 CTCGTG
BstAPI 204 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2541 GCATCTCGTGC
BstEII 1631 GGTCACC G^GT[ACGT]ACC
2153 GGTCACC
BstPI 1631 GGTCACC G^GT[ACGT]ACC
2153 GGTCACC
Bsu36I 325 CCTCAGG CC^T[ACGT]AGG
1861 CCTCAGG
DrdI 1139 GACGGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1380 GACAGCTTTGTC
Eco81I 325 CCTCAGG CC^T[ACGT]AGG
1861 CCTCAGG
EcoNI 50 CCTTTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1968 CCTGGCCAAGG
EcoO65I 1631 GGTCACC G^GT[ACGT]ACC
2153 GGTCACC
HgaI 265 GACGC GACGC(5/10)
392 GACGC
MroI 903 TCCGGA T^CCGGA
2664 TCCGGA
PflFI 616 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1579 GACTACGTC
PflMI 2130 CCAGCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3034 CCACGCCCTGG
PstI 1809 CTGCAG CTGCA^G
2577 CTGCAG
RcaI 3159 TCATGA T^CATGA
3251 TCATGA
SmlI 1263 CTTGAG C^T[CT][GA]AG
1289 CTTAAG
Tth111I 616 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1579 GACTACGTC
Van91I 2130 CCAGCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3034 CCACGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII